畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (2): 115-119.doi:

• 遗传繁育 • 上一篇    下一篇

荷斯坦牛BoLA -DRB3基因多态性及其与乳房炎抗性关系分析

张夫千;史远刚;郑小敏;唐大伟;赵鹏   

  1. 宁夏大学农学院 ,四正生物工程研究中心,银川,750021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25
  • 通讯作者: 张夫千

Research on Polymorphism of BoLA-DRB3 Gene in Holstein and the Relationship between Different Genotypes and Resistance to Dairy Mastitis

Zhang Fu-qian;Shi Yuangang;Zheng Xiaomin;Tang Dawei;Zhao Peng   

  1. Agriculture School ,Ningxia University, Syngen Bio-tech Search Center,Yinchuan,750021
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 以PCR-RFLP方法检测了BoLA-DRB3基因在荷斯坦奶牛中的多态性,并统计分析了产犊年季和BoLA-DRB3基因分别被RsaI和HaeIII酶切后的不同基因型对SCS及其它产乳性状的影响。结果表明,RsaⅠAD型的SCS显著高于RsaⅠEG型(P<0.05)。另外,年季和BoLA-DRB3被RsaⅠ酶切后的不同基因型对蛋白率和乳脂率的影响均达到了显著水平(P<0.05)。Hae III AB型个体的蛋白率显著高于Hae III AA型(P<0.05)。

Abstract: In this study, the restriction polymorphism of BoLA-DRB3 loci in Holstein was investigated by PCR-RFLP,and the effect of year-season of calving and different genotypes of BoLA-DRB3 loci respectively digested by RsaI and HaeIII was analysed. The results suggested that SCS in individuals of RsaⅠAD genotype were significantly higher than that in individuals of RsaⅠEG genotype(P<0.05); and that season of calving and different genotypes digested by RsaⅠhad significant effects on protein and fat percentage at level of P<0.05 ; and that protein percentage in individuals of Hae III AB genotype were significantly higher than that in individuals of Hae III AA genotype(P<0.05).