Abstract: PCR-SSCP and DNA sequencing techniques were used to analyze the polymorphism of partial sequence of KAP3.2 gene in 254 Tibetan sheep including three types (Plateau, Oula and Qiaoke). Based on the data, the correlation between the polymorphisms and production traits (body weight, wool length and wool yield) was studied using least square linear model The results showed that three genotypes (AA, AB and BB) were detected at KAP3.2 gene locus. Allele A was predominant in Plateau type, and allele B was predominant in Oula and Qiaoke types. Frequencies of genotype deviated from Hardy-Weinberg equilibrium in three types. The polymorphism was medium in Plateau and Oula types, while low in Qiaoke type. There was one mutation (C to T) at 271 bp on KAP3.2 gene. For the body weight, there was no significant difference among various genotypes in three types of Tibetan sheep. For the wool length, individuals with genotype AA was significantly higher (P＜0.05, P＜0.01) than those with BB genotype, and there was no significant difference between AA and AB genotypes in three types of Tibetan sheep. For the wool yield, individuals with AA genotype was significantly higher(P＜0.05) than those with AB and BB genotypes in three types of Tibetan sheep. The results indicated that the KAP3.2 could be regarded as marker for wool length and wool yield traits in Tibetan sheep.