Abstract: Liver material of a dead giant panda and different generations of CCV DXMV strain isolated from it were used to amplify partial spike protein gene of canine coronavirus by nested PCR with primers of CCVF1,CCVR1 and CCVF2,CCVR2. 1 086 bp and 515 bp nucleotide sequences were acquired respectively. The fragments were purified, sequenced and analyzed with the software of DNASTAR and DNASIS. The results showed that the sequences amplified from the giant panda liver and 2,3,29 generations of CCV DXMV between primers of CCVF2 and CCVR2 were identical and suggested that the virus was successfully isolated from the giant panda liver. 1 047 bp nucleotides between primers of CCVF1 and CCVR1 were correctly sequenced from the 3rd generation of CCV DXMV and its homology with other CCV strains were 83.3%~100%, especially 100% with the CCV NVSL strain that isolated from USA.