Abstract: The keratin family includes epithelial (soft) keratins and hair (hard) keratins, and can be divided into acidic typeⅠand basic to neutral typeⅡsubfamilies. A cDNA was library constructed with poly (A)⁺RNA from adult goat skin and 392 ESTs were analyzed. Based on sequence homology comparisons with the known typeⅠhair keratins sheep 8CⅠand hHa1, A fulllength cDNA that encoded goat hair keratin gHaⅠ(GenBank Accession No.AY510110. 1) could be identified, it derived 413 amino acid sequence comparisons with the sheep 8C1 type Ⅰ mRNA exhibits the highest homology (97.8%). In site hybridization with the gHa1 cRNA probe on skin revealed a strong expression in cortex of both primary and secondary hair follicles.