Abstract: In this study, we completed the chromosomal mapping of porcine NDUFS2 （NADH dehydrogenase (ubiquinone) Fe-S protein 2）gene using a radiation hybrid panel, cloned the coding sequence, predicted the structure and function of NDUFS2 gene with some software tools, and analyzed the expression profile of NDUFS2 gene in 11 different porcine tissues with semiquantitative RT PCR. The NDUFS2 gene was physically mapped on porcine chromosome 4q and closely linked to marker SW589. The coding sequence of porcine NDUFS2 gene is 1 392 nt, coding for 463 amino acids. The results of phylogenetic analysis among different species indicated that the porcine NDUFS2 gene is more homologous to bovine NDUFS2 gene. According to the structural prediction, porcine NDUFS2 gene has a conserved NuoD domain involved in generating and transforming of energy. The results of tissue expression profile showed that the expressive levels of NDUFS2 gene were different in 11 porcine tissues.