Abstract: Basing on five kinds of Chinese herb medicine (the dandelion, the honeysuckle, the forsythia, the root of large-flowered skullcap and the root of red-rooted salvia), the combination injection of dandelion was developed. In the study on the physical stability and quality control experiment, it showed that the color, clarity, pH were steady to the light and temperature, further more the quality was also fit the demands. The safety of the preparation was also studied. No mouse was dead in the acute oral toxicity test at a dose of 40 mL/kg b.w. to 30 mice, the LD₅₀ of enterocoelia injection in mice was 51.20 mL/kg and the 95% confidence was from 44.19 to 59.24 mL/kg. There was no local tissue irratation in the local irratation test. The injection had some repression to staphylococcus aureus and the MIC was 1/40.The result of pharmacodynamics studies showed that the injectable solution can be effectivly against the swelling of the auricle caused by xylene compared with the control group (P＜0.05). The injectable solution had a good effect to the clinical mastitis (cure rate was 48.39%, effective rate was 77%)；it also had an excellent effect to the subclinical mastitis, when the infected cow were checked on the 8th day, the natural cure rate was 12.5% of the control group, and the infected rate was 16.7%；while the cure rate was 7096% in the injection group, the newly infected rate was 0.