Abstract: Expression of phosphodiesterase (PDE) isoenzymes was detected in the skeletal muscle tissue in China experimental minature pig in order to seek predominant phosphodiesterase isoenzymes, which is related to development of muscle tissue, and to screen out the selective PDE inhibitors from Chinese herbal medicines, which can improve animal growth and productivity. In this experiment, total RNA was isolated from muscle tissue, the expression of PDE isoenzymes gene was then analyzed by reverse transcript polymerase chain reaction (RT-PCR). 16 PDE isoenzymes mRNA were detected in muscle tissues of China experimental minature pig, i.e. PDE1A, 1C, 2A, 3A, 3B, 4A, 4B, 4C, 4D, 5A, 7A, 7B, 8A, 8B, 9A and 11A except PDE1B and 10A. Among them expression of PDE1A，1C，2A，3A，4B，4C，8A and 8B mRNA have not been reported in the muscle tissue of human and other animals. The different expression extent of the 16 PDE genes were discovered from the electrophoresis bands and need to be elucidated by quantitative detection.