猪带绦虫AgB与猪CD58或IFN-γ共表达质粒的构建及其在BHK-21细胞中的表达

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (7): 694-699.doi:

猪带绦虫AgB与猪CD58或IFN-γ共表达质粒的构建及其在BHK-21细胞中的表达

侯俊玲;景志忠;郑亚东;胡志敏;骆学农;蒙学莲;窦永喜;刘军龙;才学鹏

中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室，兰州 730046

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-25 发布日期:2007-07-25

Construction of Co-expressing Plasmids of AgB from Taenia Solium with Porcine CD58 or IFN-γ Gene and Expression in BHK-21 Cells

HOU Jun-ling;JING Zhi-zhong;ZHENG Ya-dong;HU Zhi-min; LUO Xue-nong;MENG Xue-lian;DOU Yong-xi;LIU Jun-long;CAI Xue-peng

Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

摘要/Abstract

摘要： 猪囊尾蚴病是一种重要的人畜共患病，研制有效、安全、廉价的疫苗是当前主要的研究方向之一。本试验通过PCR方法，从含猪囊尾蚴B抗原（AgB）、猪CD58和猪IFN-γ的重组克隆质粒中扩增出AgB、CD58和IFN-γ基因，然后将其克隆到真核表达载体pBudCE4.1中。重组表达质粒鉴定后，在脂质体作用下转染BHK-21细胞，通过间接免疫荧光（IFA）或直接免疫荧光试验分别检测AgB、CD58和IFN-γ在BHK-21中的表达。结果显示，AgB、CD58和IFN-γ在BHK-21细胞中都成功表达，这为研制抗猪囊尾蚴DNA疫苗奠定了坚实基础。

Abstract: Cysticercosis is an important zoonosis.The development of efficient, safety and cheap vaccines is one of contemporary major directions. In this study, AgB, CD58 and IFN-γ gene were amplified from the former constructed recombinant plasmids named pGEM-TEasy-AgB, pGEM-TEasy-CD58 and pGEM-TEasy-IFN-γ by PCR and cloned into pBudCE4.1.The acquired recombinant plasmids were transfected into BHK-21 cell with liposome. 48 hours after transfection, the expression of AgB, CD58 and IFN-γ were tested by indirect immunofluorescence assay (IFA) or direct immunofluorescence assay.The results showed that AgB, CD58 and IFN-γ were successfully expressed in BHK-21 cells, providing a solid basis for development of DNA vaccine against cysticercosis

侯俊玲;景志忠;郑亚东;胡志敏;骆学农;蒙学莲;窦永喜;刘军龙;才学鹏. 猪带绦虫AgB与猪CD58或IFN-γ共表达质粒的构建及其在BHK-21细胞中的表达[J]. 畜牧兽医学报, 2007, 38(7): 694-699.

HOU Jun-ling;JING Zhi-zhong;ZHENG Ya-dong;HU Zhi-min;LUO Xue-nong;MENG Xue-lian;DOU Yong-xi;LIU Jun-long;CAI Xue-peng. Construction of Co-expressing Plasmids of AgB from Taenia Solium with Porcine CD58 or IFN-γ Gene and Expression in BHK-21 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(7): 694-699.

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