畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (9): 899-906.doi:

• 遗传繁育 • 上一篇    下一篇

绿头野鸭与我国主要地方鸭品种遗传多样性研究

苏瑛;陈国宏;龙瑞军;万江虹;马龙   

  1. 1广东海洋大学农学院,湛江 524088; 2扬州大学动物科技学院,扬州 225009;3兰州大学,兰州730070;4广东湛江金牛实业发展公司,湛江 524038;5湛江市畜牧局,湛江 524038
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-25 发布日期:2007-09-25

Study on Genetic Diversity between Mallard and Chinese Local Duck Breeds

SU Ying;CHEN Guo-hong;LONG Rui-jun;WAN Jiang-hong;MA Long5   

  1. 1Agricultural College of Guangdong Ocean University, Zhanjiang 524088, China; 2College of Animal Science and Technalogy, Yangzhou University, Yangzhou 225009, China; 3Lanzhou University, Lanzhou 730070, China; 4Guangdong Zhanjiang Jinniu Industry Co, Zhanjiang 524038,China;5Zhanjiang Farming Bureau,Zhanjiang 524038, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

摘要: 利用17个微卫星标记,采用PCR扩增,8%非变性聚丙烯酰胺凝胶电泳、银染,对绿头野鸭种群和我国9个主要地方鸭品种的遗传多样性进行了检测,统计了各群体的平均有效等位基因(Ne)、平均遗传杂合度(H)、多态信息含量(PIC)、群体间的遗传距离(Ds)和相似系数(I)。采用UPGMA和NJ法构建系统发生树。结果表明:17个微卫星座位在绿头野鸭种群和我国9个主要地方鸭品种中的多态信息含量均为高度多态,所有鸭种的平均PIC、H、Ne分别为0.650 6、0.689 6、4.1±1.5,基因多态性和遗传多样性相对较高;家鸭各品种间的遗传距离在0.163 1~0.684 4范围内,绿头野鸭与家鸭品种间的遗传距离分别在0.309 6~0.821 9之间。根据系统发育树推断中国主要地方鸭品种不是单一起源于绿头野鸭。中国主要地方鸭品种大致可以分为5支。一支为北京鸭、荆江鸭,一支为中山鸭、建昌鸭及高邮鸭,一支为靖西大麻鸭和金定鸭,连城鸭为一支,绍兴鸭为另一支。

Abstract: The genetic polymorphisms of 17 microsatellites were investigated in nine indigenous duck breeds and one species of wild duck (mallard) in China Average effective number of alleles (Ne) and average rates of homozygote of each breeds were counted. Accordingly, allele frequencies of 17 microsatellite, polyomerphism information content (PIC), mean heterozygosity (H) and genetic distances (Ds) were also calculated. Moreover, dendrograms using UPGMA and the Neighbor-joining method were produced. The nine indigenous duck breeds and one species of wild duck (mallard) population have a high average PIC(0.650 6)and H(0.689 6). The effective number of alleles is 4.1±1.5. Ds is between 0.163 1 and 0.684 4 among nine indigenous duck breeds. It is deduced that Chinese ducks have not only the same ancestor and about five diverges: 1) Beijing duck, Jingjiang duck; 2) Zhongshan, Jianchang and Gaoyou duck; 3) Jingxi duck, Jinding duck; 4) Liancheng duck; 5) Shaoxing duck