鸡IL-18真核表达载体的构建及其对新城疫疫苗免疫增强作用的研究

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (5): 476-481.doi:

鸡IL-18真核表达载体的构建及其对新城疫疫苗免疫增强作用的研究

程相朝;赵德明;吴庭才;李银聚;张春杰

1.河南科技大学动物科技学院，洛阳 471003; 2.中国农业大学动物医学院,北京 100094

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-05-25 发布日期:2005-05-25

Construction of Eukaryotic Expression Plasmids Encoding Chicken IL-18 Gene and Study on Its Immunoenhancement on Newcastle Disease Vaccine

CHENG Xiang-chao;ZHAO De-ming;WU Ting-cai;LI Yin-ju;ZHANG Chun-jie

1.Animal Science and Technology College, Henan University of Science and Technology，Luoyang 471003,China; 2. College of Veterinary Medicine, China Agricultural University ,Beijing 100094 ,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-05-25 Published:2005-05-25

摘要/Abstract

摘要： 利用首次从鸡新城疫疫苗接种的鸡胚脾细胞中扩增出的鸡IL-18全基因,构建IL-18真核表达载体（pcDNA3-IL18）,并观察其对新城疫疫苗的免疫增强作用。结果表明，同时接种pcDNA3-IL18质粒和活疫苗的免疫鸡在接种后35 d内，特别是15 d之后所产生的HI抗体效价和T、B淋巴细胞增殖反应均高于单纯疫苗免疫组及pcDNA3空白质粒和疫苗联合免疫组。其中，HI抗体效价在15 d 时差异显著（P<0.05），在30和35 d时差异极显著（P<0.01）； T淋巴细胞增殖反应在15 d后均表现为差异显著（P<0.05〉或极显著(P<0.01)；而单纯疫苗组与空白质粒和疫苗联合免疫组之间的各测定指标则无明显的差异（P>0.05）。在35 d时进行攻毒，pcDNA3-IL18和疫苗联合免疫组的保护率为83.3%,而单纯疫苗免疫组、空白质粒和疫苗联合免疫组鸡的保护率则分别只有61.5%和66.7%。说明该pcDNA3-IL18真核表达质粒在鸡体内得到了表达，表达产物不仅能够显著增强新城疫疫苗所诱导的细胞免疫和体液免疫反应，而且还可以明显提高疫苗的保护率。

Abstract: Complete chicken IL-18 gene were amplified firstly by RT-PCR from spleen cells of chicken embryo inoculated with Newcastle disease virus. The gene were inserted into pcDNA₃ vector, resulting in the construction of pcDNA₃-IL18 plasmid. Immunoenhancement effects of the pcDNA₃-IL18 plasmids on Newcastle disease vaccine were observed by the test of HI antibody, proliferation response of the T lymphocytes from thymus and the B lymphocytes from bursa of Fabricius. The results indicated that chicken IL-18 have obvious immunoenhancement effect on Newcastle disease vaccine. It can not only enhance the cell mediated immune response obviously，but also enhance the humoral response，thus improve the protective rate of ND vaccine.

程相朝;赵德明;吴庭才;李银聚;张春杰. 鸡IL-18真核表达载体的构建及其对新城疫疫苗免疫增强作用的研究 [J]. 畜牧兽医学报, 2005, 36(5): 476-481.

CHENG Xiang-chao;ZHAO De-ming;WU Ting-cai;LI Yin-ju;ZHANG Chun-jie. Construction of Eukaryotic Expression Plasmids Encoding Chicken IL-18 Gene and Study on Its Immunoenhancement on Newcastle Disease Vaccine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(5): 476-481.

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