畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (9): 1190-1195.doi:

• 遗传繁育 • 上一篇    下一篇

孕酮对体外培养的牛子宫内膜细胞上孕酮受体表达的影响

张明1,甘潇2,郑洁1,赖松家1,张华2,王雄清2,朱庆1*   

  1. 1. 四川农业大学动物科技学院,雅安 625014;2. 四川绵阳师范学院动物应用技术研究所,绵阳 621000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-24 发布日期:2008-09-24
  • 通讯作者: 朱庆

Effects of Progesterone on Progesterone Receptor Expression in Cultured Bovine Endometrial Cells (bEC)

ZHANG Ming1, GAN Xiao2, ZHENG Jie1, LAI Song-jia1
ZHANG Hua2, WANG Xiong-qing2, ZHU Qing1*
  

  1. 1College of Animal Science & Technology, Sichuan Agricultural University, Ya’an 625014, China;2.Institute of Applied Animal Technology, Mianyang Normal University, Mianyang 621000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-24 Published:2008-09-24
  • Contact: ZHU Qing

摘要: 本试验分别用50、100、200和400 ng/mL浓度的孕酮处理体外培养的牛子宫内膜细胞1、3、6、12、24 h,然后收集细胞通过半定量RT-PCR分析孕酮对子宫内膜细胞孕酮受体(PR)表达的影响。结果表明:在细胞层次水平上,孕酮能够抑制子宫内膜体外培养细胞上孕酮受体的表达。

Abstract: In the studies, bovine uterinal endometrium cells were cultured in medium including progesterone at 50, 100, 200 and 400 ng/mL respectively and collected after incubation for 1, 3, 6, 12 and 24 hours. Semiquantitative revervedtranscript polymerase chain reaction (RT-PCR) analysis was used to observe the effects of progesterone on progesterone receptor (PR) expression. Results of the present study indicate that progesterone inhibit the expression of PR in vitro.