Abstract: A 11.7 kb deletion region triggers intersexuality and polledness in goats， termed as Polled Intersex Syndrome (PIS). Through the sequence analysis of four repeated sequences (1-3 120 bp，3 988-4 565 bp，4 989-7 088 bp，7 875-9 135 bp) in goat PIS deletion region (AF404302)， we found that the 7 875-9 135 bp segment belonged to L1M3 subfamily of LINE-1 and the others were members of BDDF subfamily of RTE. Depending on the results of tblastn program searching using the fulllength protein sequences of RT domain in PIS region as query sequences against the bovine genome database，56 consensus sequences were constructed. Using the consensus sequences we deduced the formation time of PIS region at （22.5-30） million years， just the divergence time of Bovidae from Artiodactyla. Combined with other evidence， the PIS region might only exist in Bovidae， and another evidence of the role of PIS region and the non-LTR competition doctrine was provided in this paper.