硫酸化人参总皂苷对人工感染MDV鸡淋巴细胞活性的调节

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (9): 1272-1277.doi:

硫酸化人参总皂苷对人工感染MDV鸡淋巴细胞活性的调节

王鲁^1，2，王春元²，付本懂²，褚秀玲^2，3，伊鹏霏²，申海清²，韦旭斌²*

1. 贵州大学动物科学学院，贵阳 550025； 2.吉林大学畜牧兽医学院，长春 130062；3.河北北方学院动物科技学院，张家口 075131

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-24 发布日期:2008-09-24
通讯作者: 韦旭斌

Regulating Effect of Sulfated Total Saponin of Panax Ginseng on the Activity of Peripheral Lymphocyte of Chicken Infected with MDV

WANG Lu^1，2，WANG Chun-yuan²，FU Ben-dong²，CHU Xiu-ling^2，3，
YI Peng-fei²，SHEN Hai-qing²，WEI Xu-bin²*

1. College of Animal Science，Guizhou University，Guiyang 550025，China；2. College of Animal Science and Veterinary Medicine，Jilin University，Changchun 130062，China；3. College of Veterinary Medicine， North College of Hebei， Zhangjiakou 075131， China

Received:1900-01-01 Revised:1900-01-01 Online:2008-09-24 Published:2008-09-24
Contact: WEI Xu-bin

摘要/Abstract

摘要： 鸡马立克氏病病毒（MDV）经腹腔注射感染8日龄雏鸡，攻毒15 d后检测硫酸化人参总皂苷对鸡外周血白细胞数和淋巴细胞增殖活性的影响，并利用半定量RT-PCR方法分析外周血淋巴细胞IFN-γ mRNA的表达水平。结果表明，MDV感染能引起鸡淋巴细胞百分比相对增多（P＜0.01），人参总皂苷及其硫酸化人参总皂苷不能改变MDV所致变化（P＞0.05），且硫酸化人参总皂苷与人参总皂苷之间没有差异（P＞0.05）；MDV感染极显著抑制淋巴细胞增殖（P＜0.01），人参总皂苷能明显改善MDV对感染鸡淋巴细胞增殖的抑制（P＜0.01），硫酸化人参总皂苷促进了MDV所致的抑制状态（P＜0.01）；人参总皂苷及其硫酸化人参总皂苷均能增强MDV所致鸡淋巴细胞IFN-γ mRNA的表达，与健康组相比，硫酸化人参总皂苷极显著增加IFN-γ mRNA表达（P＜0.01）。结果提示，硫酸化人参总皂苷对人工感染MDV鸡的淋巴细胞活性具有调节作用，其作用比人参总皂苷的作用更强。

Abstract: Eight-day-old chickens were injected peritoneally with Marek’s disease virus (MDV) to establish MD model， and the effects of sulfated Total Saponin of Panax Ginseng(s-TSPG) on the number of leucocytes， proliferation of lymphocytes， and the expression level of IFN-γ mRNA in peripheral blood lymphocytes by semi-quantitative reverse transcription-PCR were evaluated besed on the Model at 15 days postinfection. The results showed that the percentage of lymphocytes in chicken infected by MDV was relatively increased (P＜0.01)， s-TSPG and TSPG did not change the increasing level (P＞0.05)， and there was no difference between s-TSPG and TSPG (P＞0.05). The lymphocyte proliferation were significantly depressed in chicken infected by MDV (P＜0.01)， and TSPG improved this suppression (P＜0.01)， while s-TSPG promoted this suppression (P＜0.01). The expression levels of IFN-γ mRNA from lymphocytes induced by MDV were enhanced by s-TSPG and TSPG， and the expression level of IFN-γ mRNA enhanced by s-TSPG was significantly higher than that of the control group (P＜0.01). These data suggested that the regulating effect of s-TSPG on the activity of peripheral lymphocyte of chicken infected by MDV is higher than that of TSPG.

王鲁;王春元;付本懂;褚秀玲;伊鹏霏;申海清;韦旭斌. 硫酸化人参总皂苷对人工感染MDV鸡淋巴细胞活性的调节[J]. 畜牧兽医学报, 2008, 39(9): 1272-1277.

WANG Lu;WANG Chun-yuan;FU Ben-dong;CHU Xiu-ling;YI Peng-fei;SHEN Hai-qing;WEI Xu-bin. Regulating Effect of Sulfated Total Saponin of Panax Ginseng on the Activity of Peripheral Lymphocyte of Chicken Infected with MDV[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(9): 1272-1277.

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