Abstract: 12 DLY pigs(70 kg) were randomly divided into two groups with diets of high (DE 13.81 MJ/kg, CP 14%) and low (DE 12.55 MJ/kg, CP 11%) nutritional level,respectively. All pigs were slaughtered when their weight are about 100 kg, and determined PRKAG3 gene expression, carcass characteristics and meat quality traits. The results showed that low nutritional level increased PRKAG3 gene expression(P>0.05).High nutritional level increased dressing percentage, lean percentage, loin muscle area, a, b and L values（P>0.05）, but drip loss was significantly lower than that of low nutritional level(P<0.05). PRKAG3 gene expression was positive relative to lean percentage, loin muscle area, a value, b value and drip loss（P>005）, but significantly negative relation to pH2(P<0.05). These results suggested that nutritional level influenced PRKAG3 gene expression and so influenced meat quality.