Abstract: The aim of this study was to study the correlation between animal immunization antibody titer and protection against FMDV type O challenge, dynamic detections of the antibodies in cattle immunized with the FMD type O vaccine by Colloidal gold immunochromatographic kit for detection the FMDV O serology. Vaccine testing were determined with FMDV type O inactivated vaccine quality standards, the strips were used in detecting antibody titer of immunized cattle, sheep and pigs and dynamic detections of the antibodies in cattle immunized with the FMD type O vaccine were carried out. The results showed that when immune serum dilution of cattle, pigs and sheep was greater than or equal to 1∶8, the test strip shows positive results, then the antibody levels in 99% immunized animals were in the scope of protection; if the serum dilution was less than or equal to 1∶2, the test strip display negative results, then the antibody levels of immune animals were outside the scope of protection; serum dilution was between 1∶2 and 1∶8, the test strip show positive results, then antibody levels in 50% immunized animals are in the scope of protection. In the present study, we determined the correlation between animal immunization antibody titer and protection against FMDV type O challenge. The results showed that the boosterimmunization on eighth week post primary immunization was the most favorable procedure.