瘦蛋白对体外培养的新生牛脂肪细胞Ob-Rb mRNA表达的影响

畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (11): 1149-1153.doi:

瘦蛋白对体外培养的新生牛脂肪细胞Ob-Rb mRNA表达的影响

牛淑玲;张才;夏成;王哲;梁冠生;徐闯

1吉林大学畜牧兽医学院, 长春 130062 ；2黑龙江八一农垦大学, 大庆 163319

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-11-25 发布日期:2006-11-25

Effects of Leptin on the Expression of Ob-Rb mRNA in the Cultured Adipocytes of Newborn Calf

Received:1900-01-01 Revised:1900-01-01 Online:2006-11-25 Published:2006-11-25

摘要/Abstract

摘要： 取单层生长良好的脂肪细胞,采用单因素试验,分别添加浓度为5 ng/mL外源牛重组瘦蛋白（每12 h添加1次）不添加作为对照至4、12、24、36、48和72 h后提取总RNA，每个处理3个重复(孔)，应用竞争RTPCR法检测外源瘦蛋白对初生犊牛脂肪细胞Leptin长型受体(Ob-Rb)表达水平的影响，结果与对照组相比在12~24 h内随着培养时间的增加，脂肪细胞Ob-Rb表达水平量亦显著增加（P<0.01），之后其表达量逐渐回降，在48～72 h趋于平稳（P>0.05）。结果表明，在一定时间内瘦蛋白可提高体外培养的新生牛脂肪细胞Ob-Rb mRNA的表达水平。

Abstract: A single factor duplicate test was designed to investigate whether additional leptin(5 ng/mL·12 h) in adipose cell cultured medium at 12,24,36,48 and 72 hours(12 hour each)has a role on ObRb mRNA expression by competitivePCR. The result showed that the quantity of ObRb mRNA in adipose cell cultured for 12～24 h was significantly higher in the treated groups than that of control group（P<0.01）.Following gradual decreasion ,the expression of ObRb kept a stable level from 48 to 72 h（P>0.05）.It considered that leptin could accelerate the expression of ObRb in the cultured adipose cells with giveen time.

牛淑玲;张才;夏成;王哲;梁冠生;徐闯. 瘦蛋白对体外培养的新生牛脂肪细胞Ob-Rb mRNA表达的影响[J]. 畜牧兽医学报, 2006, 37(11): 1149-1153.

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