Abstract: In order to explore the interaction and balance relationship between substances in the neuro-endocrine-immunoregulatory network, we researched the coexistence of the IFN-γR and ER immunoreactivity(ir) in the hypothalamus of healthy adult female SD rat by immunohistochemistry method.The results were as follows: there were IFN-γR-ir and ER-ir single and double labeled cells in the hypothalamus. The double labeled cells were mainly located in the suprachiasmatic nucleus, anteromedial nucleus, anterolateral nucleus, periventricular nucleus, supraoptic nucleus, periventricular nucleus, nuclei of anterior hypothalamic nucleus, poster hypothalamic nucleus, ventral premammillary nucleus, dorsal premammillary nucleus, and so on. IFN-γR/ER double labelled cells accounted for about 60.9% of total labeled cells.The IFNγR-ir producs were blue-black, which were mainly located in the cytoplasm. The ER-ir products were brown in color, which were mainly located in the whole neurons. These results implied that there were interaction between the IFN-γR and ER in the neuro-endocrine-immunoregulatory network, which probably join in the action of the neuro-endocrine-immunoregulatory network.