Abstract: Four castrated Small Tail Han ram fitted with permanent ruminal, duodenal, and ileal fistula were used to examine the feasibility of a non-isotopic dual-marker method to measure duodenal and ileal digesta flow. The sheep were fed pellet diet by automatic feeder. For measuring digesta flow, chromic oxide (Cr₂O₃) and polyethylene glycol-4000 (PEG-4000) were added in the pellet diet. Markers were directly put into the rumen through rumen fistula when measuring their recovery rate in feces. The recovery rate of chromic oxide and PEG measured in the present study was 95.75% and 91.48% respectively. The concentrations of chromic oxide and PEG in ruminal, duodenal and ileal whole digesta and fluid phases reached plateau after about 4 d successive feeding of marker-containing pellets. The averages of duodenal and ileal digesta flow rates measured in present study were 10.054 3±1.726 5 L/d and 5.629 5±0.901 9 L/d respectively. The mean retention time of chromic oxide in the rumen was 88 hours, and the mean retention time of PEG was 40 hours.