Abstract: In order to explore the molecular epidemiology of subgenotype Ⅶd Newcastle disease virus (NDV) in Jiangsu Province, 16 NDVs isolated from different regions in 2008 were characterized phylogenetically. Fusion (F) and hemagglutininneuraminidase (HN) genes were amplified by RTPCR and sequenced. The phylogenetic trees based on the sequences of the F and HN genes, respectively, were almost identical. These results suggesting that no recombination was present among these NDVs. In addition, the isolation frequency of the variant strain with E347K mutation on HN increased significantly in more recent years. More attention should be paid to the emergence of the variant genotype Ⅶd NDV strains.