藏系绵羊群体遗传多样性及遗传结构分析

doi:10.11843/j.issn.0366-6964.2019.06.004

摘要/Abstract

摘要： 旨在从分子层面对藏区绵羊群体进行群体关系研究。本试验选取西藏、青海、甘肃、云南和四川5个地区的20个藏系绵羊群体共384个个体进行Affymetrix Ovine 600K芯片扫描分型，基于杂合度和近交系数分析藏系绵羊群体的遗传多样性，并通过PCA、NJ-tree和STRUCTURE分析藏系绵羊的群体聚类情况。结果发现，西藏浪卡子绵羊的观测杂合度最低（0.235 0），近交系数最高（0.279 6）；青海山谷型藏羊的观测杂合度最高（0.323 4），近交系数最低（0.009 0）。遗传结构分析结果显示，西藏和云南地区藏系绵羊能够单独聚成一支，而青海、甘肃和四川地区的草地型绵羊混乱聚集在一起。结果表明，西藏和云南地区藏系绵羊的遗传多样性普遍低于青海、甘肃和四川地区的藏系绵羊，且西藏和云南地区的藏系绵羊基本能保持地域特异性，而青海、甘肃和四川地区的草地型绵羊在遗传距离和群体结构中差异不大。

Abstract: This experiment was conducted to study population relationship of Tibetan sheep at the molecular level. A total of 384 individuals from 20 Tibetan sheep populations from 5 regions including Tibet, Qinghai, Gansu, Yunnan and Sichuan were selected for Affymetrix Ovine 600K chip scanning. The genetic diversity of Tibetan sheep populations was analyzed based on heterozygosity and inbreeding coefficient, and the population clustering of Tibetan sheep was analyzed by PCA, NJ-tree and STRUCTURE. The results showed that the observed heterozygosity of Tibet Langkazi sheep was the lowest (0.235 0) and the inbreeding coefficient was the highest (0.279 6). The observed heterozygosity of Qinghai valley type Tibetan sheep was the highest (0.323 4) and the inbreeding coefficient was the lowest (0.009 0). The results of genetic structure analysis showed that Tibetan sheep in Tibet and Yunnan could be gathered together alone, while grassland sheep in Qinghai, Gansu and Sichuan were clustered together in confusion. The results indicate that the genetic diversity of Tibetan sheep in Tibet and Yunnan is generally lower than that of Tibetan sheep in Qinghai, Gansu and Sichuan. Tibetan sheep in Tibet and Yunnan can maintain regional specificity, while grassland sheep in Qinghai, Gansu and Sichuan regions have little difference in genetic distance and population structure.

中图分类号:

S826.2

胡亮, 孙伟, 马月辉. 藏系绵羊群体遗传多样性及遗传结构分析[J]. 畜牧兽医学报, 2019, 50(6): 1145-1153.

HU Liang, SUN Wei, MA Yuehui. Study on Genetic Diversity and Genetic Structure of Tibetan Sheep Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1145-1153.

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