胰岛素样生长因子系统基因在不同鸭种发育早期肝和肌肉中的表达

doi:10.11843/j.issn.0366-6964.2014.02.003

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (2): 183-190.doi: 10.11843/j.issn.0366-6964.2014.02.003

胰岛素样生长因子系统基因在不同鸭种发育早期肝和肌肉中的表达

束婧婷^1#，朱文奇^2#，单艳菊¹，陈文峰²，徐文娟¹，胡艳¹，宋迟¹，李慧芳^1*，王金玉²

（1.江苏省家禽科学研究所，扬州 225003； 2.扬州大学动物科技学院，扬州 225009；3.江苏腾达源农牧有限公司，姜堰 225538）

收稿日期:2013-07-08 出版日期:2014-02-23 发布日期:2014-02-23
通讯作者: 李慧芳，博士，研究员，主要从事家禽遗传育种研究，Tel：0514-85599266，E-mail：feixiong@yzu.edu.cn
作者简介:束婧婷（1980-），女，江苏泗阳人，博士，副研究员，主要从事家禽分子育种研究，Tel：0514-85599179，E-mail：shujingting@163.com；朱文奇（1981-），男，江苏苏州人，博士生，助理研究员，主要从事家禽遗传育种研究，E-mail：zwqjs@163.com，#为并列第一作者
基金资助:
国家自然科学基金（31172194）；国家农业科技成果转化项目（2012GB2C100156）；江苏省科技支撑计划项目（BE2011329）；现代农业品种创新项目（CX(11)1030）

Expression of Insulin-like Growth Factor System Genes in Liver and Breast Muscle Tissue during Early Development of Different Duck Breeds

SHU Jing-ting^1#，ZHU Wen-qi^2#，SHAN Yan-ju¹，CHEN Wen-feng²，XU Wen-juan¹，HU Yan¹，SONG Chi¹，LI Hui-fang^1*，WANG Jin-yu²

（1.Jiangsu Institute of Poultry Science，Yangzhou 225003, China； 2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China； 3.Jiangsu Tengdayuang Co.Ltd，Jiangyan 225538, China)

Received:2013-07-08 Online:2014-02-23 Published:2014-02-23

摘要/Abstract

摘要：

本研究选择生长速度不同的高邮鸭和金定鸭为试验模型，首次对不同品种鸭胚胎期和出雏早期肌肉和肝中胰岛素样生长因子（Insulin-like growth factors，IGFs)系统基因的表达及其与体重等指标进行了相关性研究。采用实时荧光定量PCR方法研究鸭13、17、21、25、27胚龄和出雏后7日龄IGF-I、IGF-IR和IGFBP-3基因表达的发育性变化。结果首次证实鸭肌肉和肝IGF-I、IGF-IR和IGFBP-3在13胚龄已有表达，并表现出显著的品种和时间特异性。2品种肝中IGF-I的表达量在整个胚胎期均维持在极低的水平，出雏后显著上升；而肌肉中IGF-I的表达量要显著地高于肝。IGF-IR基因在肝中表达量高于肌肉组织，同时在肌肉中表达模式存在品种差异。IGFBP-3在2品种肝中的表达量相对较低，在胸肌中的表达量从胚胎发育末期至出雏早期均表现出上升趋势。IGF-I与IGF-IR、IGFBP-3基因表达水平之间存在负相关关系，而IGFBP-3基因与IGF-IR基因表达水平之间存在极显著的正相关；IGFs系统基因与体重、肝重和胸肌重之间呈现不同程度的线性相关。上述结果首次揭示了鸭胚胎期和出雏早期生长发育过程中IGFs系统基因表达的发育性变化模式和品种差异，同时也为深入研究IGFs系统基因的相互调控机制提供了基础数据。

Abstract:

In the present study，expression of IGF-I，IGF type-I receptor (IGF-IR)，and IGF binding protein (IGFBP)-3 genes was quantified by RT-PCR in the liver and muscle tissues on days 13，17，21，25，27 of embryonic development，as well as at 7 days post-hatching (pH) in Gaoyou and Jinding ducks differing in growth rates.The results showed that IGF-I, IGF-IR and IGFBP3 mRNA could be detected as early as on E 13 d in ducks，and showed an extremely significant breed and development differential diathesis.The expression level of IGF-I was very low throughout embryonic development before increasing dramatically by E 27 d and 7 days pH in both duck breeds.In contrast，IGF-I was expressed in muscle at much higher levels than liver throughout development.Expression of liver IGF-IR was higher than that in muscle，and differed between breeds in breast muscle.IGFBP-3 mRNA expression in liver was relatively low in both duck breeds，while in breast muscle increased gradually from the late of the embryonic period to the early post-hatching.Inverse relationships were observed for the expression of IGF-I and IGF-IR，and IGF-I and IGFBP-3，while positive relationship was observed for the expression of IGF-IR and IGFBP-3，meanwhile varying degrees of linear correlation was found between insulin-like growth factor system genes mRNA expression and body weight，liver weight and breast weight in duck.The data indicates differential expression of selected genes that comprise the IGF system in the duck liver tissue during embryonic and early pH growth and development，and provide some data for further study on the mutual regulation mechanism of genes in IGFs system.

中图分类号:

S834.2

束婧婷，朱文奇，单艳菊，陈文峰，徐文娟，胡艳，宋迟，李慧芳，王金玉. 胰岛素样生长因子系统基因在不同鸭种发育早期肝和肌肉中的表达[J]. 畜牧兽医学报, 2014, 45(2): 183-190.

SHU Jing-ting，ZHU Wen-qi，SHAN Yan-ju，CHEN Wen-feng，XU Wen-juan，HU Yan，SONG Chi，LI Hui-fang，WANG Jin-yu. Expression of Insulin-like Growth Factor System Genes in Liver and Breast Muscle Tissue during Early Development of Different Duck Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 183-190.

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胰岛素样生长因子系统基因在不同鸭种发育早期肝和肌肉中的表达

Expression of Insulin-like Growth Factor System Genes in Liver and Breast Muscle Tissue during Early Development of Different Duck Breeds

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