With the further development of nutriology and genetics，more and more concerns have been raised on new subjects such as nutrigenomics，nutrigenetics and epigenetics.These subjects comprehensively consider the effect of nutrient and genetic factors on the regulation of animal growth and development，and further explore the growth and development mechanism of animals.This paper focused on the mechanism of DNA methylation and the influences of nutrients on DNA methylation during growth and development in animals.

Of the many members which belong to the family Picornaviridae，only equine rhinitis A virus and equine rhinitis B virus are known to infect horses.The high seroprevalence of equine rhinitis viruses in horse populations contrasts with poor understanding of their contribution to disease.This review examines the current knowledge regarding their distribution，pathogenesis and recent advances in diagnostic methods.

The objectives of the present study were to identify the FGFs mRNA and protein differentially expression at different stages of hair follicle growth and development，and also to clarify the relationship between them.Firstly，gene chips were used to screen the differentially expressed genes in hair follicle at different stages and parts，FGF10，FGF18 and FGFR3,belonging to FGFs family，were identified.The qRT-PCR were used to verify the accuracy of gene chips results.Then by two-dimensional gel electrophoresis (2-DE) and mass-spectrometric technique (MS) technologies，differentially expressed proteins were identified，including FGF10，FGF18 and FGFR3 proteins.The results showed FGF10 mRNA expression level was higher in abdomen than that in neck，suggested that it might play the negative regulation role in hair follicle development，and the expression of FGF10 protein was adverse.FGF18 mRNA expression level in abdomen was higher at anagen stage and protein expression was lower in neck，suggested it might inhibit the growth of hair follicles.FGFR3 had no consistent rules.These results showed that the expression of the 3 genes at mRNA and protein levels are different in hair follicle at different stages and parts，which indicate the 3 genes are related to the growth and development of hair follicle.The results may provide basic theory for understanding molecular mechanisms of FGFs regulating hair follicle growth and development，and also provide candidate genes for fine-wool breeding project.

Genome-wide association studies (GWAS) based on high throughput SNPs genotyping technology provided basis for exploring genes associated with fertility traits in Chinese Holsteins.Data of 19 111 heifers with calving records over a period of 10 years (2001-2011) were collected from Sanyuanlühe Dairy Cattle Breeding Center.Firstly，by AI-REML combined EM algorithm，DMU software (v 6.0) was used to estimate genetic parameters for age at first calving (AFC) based on animal model.Then GWAS was performed by the software PLINK (v 1.07) on the basis of the EBVs of calving traits of 2 172 Chinese Holstein heifers to identify genes affecting the fertility traits using the Illumina BovineSNPs50 BeadChip.The results showed that 1 700 SNPs was significantly associated with AFC at genome-wide level.Preliminarily，43 significant SNPs with P-valves smaller than 10^-15 were chosen to do the bioinformatics analyses.Among the 43 SNPs，12 were located on X chromosome，while some were within 1 Mb（20.42-21.52 Mb）on SSC7.In particular，the candidate genes of KLHL4，TRAM1，TRAM2，ZNF438 and MATK were related to some reproductive disturbances or diseases.The current study explored some novel SNPs as well as potential candidate genes associated with heifer calving traits，which can be used to unravel the genetic foundation of fertility traits in dairy cattle.

The research was conducted to detect the activities of the bovine ATP5B dual luciferase report gene promoter recombinant plasmid by transfecting into 3T3L-1cells and its relative expression in 16 different tissues.Dual luciferase report gene vectors of bovine ATP5B gene promoter were transfected into 3T3L-1 cells by LPS，then the activities of 7 recombined plasmids were measured，the possible core promoter was further confirmed by bioinformatics software.The RNA of 16 different tissues in Qinchuan cattle was extracted by kit.After reverse transcription，the cDNA was tested，then ATP5B gene real-time quantitative primers and a pair of β-actin internal primers were designed.Finally，the proximal ATP5B gene promoter and the amino acid sequence of different species were analyzed by software.Luciferase activity assay indicated that pATP5B-462 had the highest Luciferase activity compared to pGL3-Basic，sequence analyzing indicated that promoter was in the regions of -547--230 bp，including TATA box，CAAT box，GATA box，v-Myb，deltaE.The analysis of ATP5B gene expression in Qinchuan bull showed that，the expression levels in the hind leg muscle and longissimus muscle were relatively high，in testosterone fat，heart，stomach，rennet，large intestine，small intestine，back fat，kidney and liver were relatively moderate，in reticulum，spleen，lung，caecum and rumen were relatively low.According to the proximal promoter，amino acid sequence alignment and the results of software analysis，ATP5B proximal promoter of bovine，goat,sheep,mice，pigs and human had higher similarity.The similarity of amino acids for ATP5B coding among goat，sheep，mice，human and pig were as high as 99%，98%，96%，96%，91%.This research confirmed the promoter region，got tissues expression profiles of bovine ATP5B gene，the results of software alignment revealed that this is a very conservative gene，this lays a foundation for further research on ATP5B gene transcription regulation and function.

In order to investigate quality characteristic of different muscles from plateau yak，the cooking loss，drip loss，Warner-Bratzler shearing force，pH value，L* value，a* value and b* value of supraspinatus (SU)，psoas major (PM)，latissimus dorsi (LA)，longissimus dorsi (LD)，biceps femoris (BF)，semitendinosus (ST)，semimembranosus (SM) and quadriceps femoris (QF) from yak carcass were determined.It was found that there were significant differences between different muscles in quality traits except a* value (P<0.05).The PM，LA and ST had significantly higher tenderness comparing to other muscles (P<0.05) with lower shearing force values than 6 kg，while the water holding capacity of LA，ST，SM was poorer than other muscles.The ST had highest lightness and yellowness (L* values and b* values) among all muscles.The LD and ST had similar quality characteristic，while the BF，SM and QF had similar quality characteristic.There was significantly negative correlation between pH values and cooking loss，which indicated that the difference of water holding capacity among different muscles could be related to level of acidification.It was shown that different parts of muscle had significant effect on yak beef quality，and difference of water holding capacity could be attributed to level of acidification.

 This study was conducted to investigate the effects of tauro ursodeoxychollc acid (TUDCA) on the development of bovine in vitro fertilization (IVF) embryos.Firstly，the splicing of XBP-1 mRNA of the embryos at different developmental stages was detected with RT-PCR.Then，the bovine IVF embryos were cultured in the medium containing different concentration of TUDCA（0，100，250，500，1 000 μmol•L^-1），respectively，and the embryonic cleavage rate，blastocyst rate，the blastocyst cell number as well as apoptosis rate of blastocyst were investigated.Meanwhile，the expression of endoplasmic reticulum stress (ERS) related genes were detected by qRT-PCR.The results showed that：XBP-1 mRNA splicing was observed during embryonic development，especially in 4-cell，morula and blastocyst stages.No significant effect were observed in the cleavage rate of IVF embryos after treating with different concentration of TUDCA，while in the group treated with 500 μmol•L^-1 TUDCA，the blastocyst rate as well as the cell number of blastocyst were increased significantly (P<0.05)，moreover，the cells apoptosis rate of blastocysts in this group was decreased significantly (P<0.05).The results of qRT-PCR showed that 500 μmol•L^-1TUDCA supplemented into the culture medium for bovine IVF embryos could significantly down-regulate the expression level of Chop gene and up-regulate Bcl-2 gene (P<0.05)，however，the expressions of Grp78 and Bax gene were no significant difference as compared to that of control group (P>0.05).These results indicated that ERS was present in the process of bovine embryos in vitro culture(IVC)，and supplementing with 500 μmol•L^-1TUDCA into the embryo culture medium might decrease ERS，and promote embryonic development.

Sheep phospholipase C zeta (PLCζ) was used to activate sheep oocytes，to explore whether PLCζ can be used as a new kind of oocyte activator.A pEGFP-N1-PLCζ recombinant plasmid were constructed and then injected into MⅡ sheep oocytes.The effect of PLCζ on activation of oocytes and embryo development were observed and counted.The results showed that pEGFP-N1-PLCζ recombinant plasmid could express PLCζ in oocytes，and could spontaneously activate parthenogenetic development of sheep oocytes.

Utilizing the gradient method，this experiment was conducted to investigate the effects of different feeding levels on digestibility of main nutrients in Dorper × Small-tailed Han crossbred ewes during non-pregnancy and lactation.Fifteen healthy ewes were selected for estrus synchronization，after parturition，these ewes were assigned into 3 groups according to the weight with randomized block design，one group in ad libitum feeding，and the other 2 groups in eighty percents (80%) or sixty percents (60%) of ad libitum feeding.In addition，nine non-pregnant ewes at the same age and breed were selected into blank group.Digestibility trials were performed on the 20，50 and 80 day of lactation，to determine the digestion characteristic of main nutrients.The results showed that the apparent digestibilities of neutral detergent fiber(NDF)，acid detergent fiber (ADF) and nitrogen(N) of non-pregnant and lactating ewes significantly increased with decreasing feeding levels (P<0.05).There was a declining trend in milk nitrogen with decreasing feeding levels (P>0.05)，but it significantly decreased with lactation progress (P<0.05).The nitrogen retention (RN)，RN/NI (nitrogen intake) and RN/DN (digested nitrogen) decreased significantly with the decreasing feeding levels (P<0.05).The apparent digestibilities of nitrogen in lactation were significantly higher than that in non-pregnancy (P<0.05)，what is more，there was a declining trend with lactation progress.The apparent digestibilities of nitrogen during the period of non-pregnancy，20，50 and 80 day of lactation were 45.21%-51.34%,73.72%-82.71%,72.79%-80.51%，73.57%-76.47%，respectively.Feeding levels significantly influence the main nutrient digestion and excretion of Dorper × Small-tailed Han crossbred ewes during non-pregnant and lactating (P<0.05).Lactation significantly improves the digestion and utilization of nutrients (P<0.05).

The objective of this study was to investigate the effects of Cysteamine hydrochloride on apparent nutrient digestibility，serum biochemical and antioxidant indices of lactation Holstein dairy cows.Forty healthy Holstein dairy cows，similarly in milk yield，days in lactation and parity，were allocated into 5 groups in a randomized complete block design with 8 cows per group.Cows were fed with supplementation of CSH for 0 (control group)，15，30，75 and 150 g in the basal diet.The pre-experimental period lasted for 2 weeks，and the experimental period lasted for 8 weeks.Apparent nutrient digestibility，serum biochemical indices，enzyme and antioxidant indices were detected.The results showed as follows：The apparent digestibility of CP，Ca，P，NDF and GE in 15 g group were higher than those in the control group (P>0.05)，however，decreased tendency was found in 75 and 150 g groups，and those in the 150 g group were the lowest.Compared to the 150 g group，15 g group significantly increased the GLOB content (P<0.05).The ALT concentration increased in 150 g group compared with the control，15 and 30 g groups (P<0.05).The SOD level decreased with the increasing doses of CSH (0.05<P<0.10).Furthermore，the GSH-PX concentration significantly increased in the 15 g group compared with control group and 150 g group (P<0.05).The MDA level of the 30-150 g groups were significantly higher than that of the 15 g group(P<0.05).Results of the present study indicated that the safe supplemental level of CSH is within 30 g.

This experiment was conducted to compare the pathogenicity，immune suppression，and the horizontal transmission capacity between intramuscular inoculation in 1-day-old chicks and intravenous inoculation in embryos with chicken anemia virus (CAV) strain SDLY08.SPF chickens were divided into four groups：chickens from embryos inoculated intravenously with CAV at 10 days of incubation，1 day-old SPF chicken inoculated with CAV intramuscularly，contact infection group and the control group.The Ratios of thymus and the Bursa to body weigh，indexes of hematology，and CAV antibody levels were examined at 14 and 42 days of age.All groups were immunized with inactivated vaccines to avian influenza virus (AIV-H9N2) and Newcastle disease virus (NDV) at 2 weeks of age，and antibody titers to AIV-H9N2 and NDV were tested at 28，35 and 42 days of age.Results indicated that both CAV-inoculated groups showed growth retardation，severe atrophy of thymus，significant decrease of HI antibody titers to NDV and H9N2，lower levels of red blood cells，white blood cells and the haematocrit (P<0.05) when compared to the control group，but there was no significant difference in these parameters between chicken groups inoculated with different routes (P<0.05).However，chicken group with embryo inoculation demonstrated some mortality (4/25) by 28 days of age and there was no mortality (0/25) in the group inoculated muscularly.Contact infection also induced growth retardation，immunosuppression and anemia，but not as strong as direct inoculation with CAV (P<0.05).At 42 days of age，all chickens inoculated muscularly at 1 day or in embryos developed antibodies positive to CAV，and 95% of in contact infected birds were also antibody positive to CAV， indicating its effective horizontal transmission.

This experiment was conducted to prepare the antiserum of antigenic domain region of duck enteritis virus (DEV) gB gene，and showed its initial application as detection reagent to detect DEV.Specific primers were designed for DEV C-KCE ul27 gene according to the cloned gene sequence of our laboratory，then the antigenic domain region of DEV gB gene （331-570 bp）was cloned by PCR which named gB-AD.Recombinant gB-AD region protein was expressed in prokaryotic expression system，pET-32a(+) / Rosetta(DE3)pLysS，by induction of IPTG and was purified by Ni-NTA column affinity chromatography，and the antigenicity of recombinant protein was identified by using the DEV positive serum.The purified recombinant protein was retrieved by cutting the gel slices that contain the right bands，and was used to immunize rabbits as immunogen to prepare the rabbit anti-gB-AD region serum.Results were as follows：The recombinant protein could be identified by DEV positive serum specifically，and the titer of polyclonal antibody for immunizing rabbit was 1∶10 240 by I-ELISA with neutralizing activity.The quality of the DEV gB molecule was about 66 kD reacted to polyclonal antibody in the reducing SDS-PAGE，on the contrary，it was about 120 and 66 kD in the non-reducing SDS-PAGE.I-ELISA and Western blot analysis showed that antibody for immunizing rabbit could identify recombinant gB-AD region protein from the infected cells respectively.In the further，indirect immunofluorescence test and neutralization assay confirmed that the antiserum could identify DEV specifically with neutralization.Above all，the results showed that antiserum for immunizing rabbit can be used as detection reagent to detect DEV，and it afforded significant data for the study on the detection of DEV and function of DEV gB.

Epitope polypeptides of peste des petits ruminants virus (PPRV) were synthesized and its immunogenicity was examined in mice.Four epitope polypeptides against F and H gene of PPRV were selected by the epitope prediction software and acquired by chemical synthesis，then were administered subcutaneously at dose of 100 μg to BALB/c mice，and the epitope antigenicity was analyzed by immunoassays of ELISA，MTT，the killing activity of NK cell and T lymphocyte subsets detection.As a result，H242-255，F528-541 and H349-362 had better humoral immune function among the four epitope polypeptides，while H242-255 and H392-405 had better cellular immune function.Thus，peptide of H242-255 can satisfy the two immunologic functions，and can be used as a candidate epitope vaccine component.The study had great significance by screening efficient epitopes for the development of PPRV epitope vaccines.

 In this study，Hetian sheep and Chinese Merino sheep were chosen as the research object.They were randomly divided into infection group (n=5) and control group (n=3).Every sheep in infection group was orally infected with 250 metacercariaes of Fasciola hepatica，the biochemical index，cytokines (IFN-γ，IL-2，IL-10，TNF-α) and immunoglobulin (IgG，IgM and IgA) in blood were measured at 0，3，6，10 weeks after infection.In Hetian sheep and Chinese Merino sheep，the content of the aspertate aminotransferase (AST)，total bilirubin (TB) and alkaline phosphatase (ALP) in serum of the infection group were significantly increased，significantly higher than that of the control group at 6 weeks after infection (P<0.05)，the serum globulin (GLB) content of the infection group in Chinese merino sheep were significantly higher than that of the control group at 6 and 10 weeks after infection (P<0.05).The serum IgM content of the infection group in Hetian sheep were significantly higher than that of control group at 6 weeks after infection (P<0.05).At 6 and 10 weeks after infection，the level of IgG，IL-2 and IFN-γ were significantly increased，significantly higher than of the control group (P<0.05).The level of serum AST，ALP in Chinese Merino sheep increased more than that of Hetian Sheep，however，the levels of serum IgM，IgG，TNF-α and IFN-γ in Chinese Merino sheep increased more than that of Hetian Sheep.The results suggest that there are some differences on immune response against Fasciola hepatica between Hetian sheep and Chinese Merino sheep，the study provides some theoretical data for sheep anti-Fasciola disease research.

 The genetic variability of Echinococcus granulosus was investigated to uncover the genetic structure in the Tibet plateau，China.The complete mitochondrial cox1 genes about 1 609 bp from 47 isolates of E.granulosus collected from populations in the Tibet plateau were sequenced.Further sequences (retrieved from NCBI database) for the cox1 gene from the Tibet plateau and the Middle East were compared with the sequences analyzed in this study.The BLAST analysis of the sequences indicated 47 isolates belonged to G1 genetype (E.granulosus sensu stricto) and 10 haplotypes (C1-C10) were detected.The major haplotype C1 in the Tibet plateau showed a 100% homology with the reference haplotype in the Middle East and presented a worldwide distribution.The haplotype diversity (Hd) and nucleotide diversity (π) observed in the Tibet plateau were both lower than these in Middle East.The average negative values of Tajima's D and Fu’s Fs were negative in the two geographic populations and the populations were not genetically differentiated.These results suggest that E.granulosus in the Tibet plateau occurred demographic expansion or bottleneck effects after the introduction of founder haplotype in the Middle East.The population was developed under the geographical isolation.

To investigate the differential expressions of vitellogenin，immunosuppressive ovarian message protein，tyrosine-protein kinase and serine/threonine-protein phosphatase genes in germinal tissues of Toxocara canis，which were designated as Tc-vit，Tc-iom，Tc-tpk and Tc-stp，the SYBR Green I real-time quantitative PCR methods were applied to analyze the different transcription of these genes in ovary，oviduct and uterus of female adult T.canis，spermary，seminal vesicle and vas deferens of male adult T.canis.The quantitative results showed that Tc-vit was transcribed in all the gremline tissues except for vas eferens；Tc-iom was transcribed in all the female germinal tissues and spermary，seminal vesicle，but not in vas deferens；the transcription of Tc-tpk in male sperm-production tissues were higher than that of female tissues；Tc-stp was highly transcribed in spermary and vas deferens and opposite in all the female germline tissues.These results laid down the foundation for the further research of gender-related genes of T.canis.

 In order to establish living measurable evaluation model for E.tenella resistance and to explore new coccidiosis-resistance breeding method for chickens，Jinghai Yellow Chickens were selected to compare the difference of coccidiosis resistance parameters got from related studies between infected and control group，valid parameters were selected to perform principal component analysis，optimizing comprehensive evaluation model was selected by the significant correlating coefficients between coccidiosis resistance parameters and principal component functions.There were 6 parameters which had significant difference between infected and control group among the 14 living measurable parameters，they were weight gain in the 3rd-5th day post infection，the concentration of CAT，GSH-PX，MDA，SOD and IFN-γ at the 8th day post infection.Six principal components and one accumulated contribution up to 80% comprehensive resistance evaluating models were established by principal component analysis.The results of correlation analysis showed that the first principal component model were significant or highly significantly related to 9 resistance parameters (P＜0.01 or P＜0.05)，especially to caecum lesion (P＜0.01)，the rest of each principal component models were only related to 2-3 parameters (P＜0.01 or P＜0.05)，and not to caecum lesion (P＞0.05).The distribution of the first principal component model was skewed positively，the order of selection index value calculated from the optimizing model were in accordance with caecum lesion performance.The optimizing model of f_i1=-0.64Zx_i1+0.31Zx_i2+0.80Zx_i3-0.05Zx_i4-0.08Zx_i5+0.59Zx_i6 might be the best coccidiosis resistance comprehensive selection index model and could be used for chicken resistance selection breeding.

To study the pathogenicity of duck Tembusu virus in Cherry Valley ducklings，infection model was established by inoculating Tembusu virus FX 2010 strain intramuscularly into one-week-old ducklings to dynamically supervise clinical symptoms，pathological changes，tissue viral loads and immune responses of infected ducklings.The ducklings showed loss of appetite and yellow-white stools at the third day post infection (3 dpi) and neurological symptoms at 5 dpi.In addition，some ducklings died acutely and the mortality rate was 22.5% (9/40).Necropsy results showed endocardial hemorrhage，swelling and necrosis in spleen.The liver and kidney were degenerative and swelling and meningeal congestion.Histopathological changes in infected ducklings mainly presented as degeneration and necrosis in parenchymal cells of heart，liver and kidney，interstitial inflammatory cells were infiltrated or bleeding，focal lymphocytes necrosis and a large number of heterophilic granulocytes infiltration in spleen，typical viral encephalitis in brain.Tembusu virus could be detected in every organ of infected ducklings at 1 dpi and viral levels reached a peak at 3 dpi in all organs except for the spleen.The levels of serum neutralizing antibodies were low at 5 dpi，then gradually increased and reached the peaks till at dpi.These results suggest that duck Tembusu virus could quickly invade tissues and numerously replicate in affected ducklings，characterized by pantropic virus injuring most tissues.Severe ducklings usually died of acute septicemia.The neutralizing antibodies could be rapidly produced to fight against infection and eliminate the virus.

The experiment was conducted to compare the difference of antibiotic and copper resistance among E.coli isolated from sows and piglets before and after weaning.In the experiment，224 of E.coil were isolated from lactation sows，suckling and weaned piglets，which antibiotic resistance to 7 antimicrobial agents and copper resistance was detected using agar dilution method according to the recommendation of the CLSI.The remarkable antibiotic resistance of isolated E.coil to 7 selected antibiotics was observed，the percentage of resistance isolates to individual antibiotic was ranged from 28.13% to 93.30%.70.09% of all isolates possessed multi-drug resistance (MDR).The antibiotic resistance of E.coil isolates from sows and piglets was highly similar，except resistance to ampicillin and gentamicin.However，the antibiotic resistance of isolates from weaned pigs changed significantly.The antibiotic resistance to ceftiofur，florfenicol and chloramphenicol increased very significantly (P<0.01)，resistance to ampicillin increased significantly (P<0.05).Comparing to sow and suckling piglets，the copper resistance of isolates from weaned pigs increased very significantly (P<0.01).In addition，when the copper resistance of E.coil isolates changed，the antibiotic resistance also changed，there might be a correlation between copper and antibiotic resistance.Drug-resistance bacteria in the gut of sows and in feed antibiotics contribute significantly to the development of antibiotics of E.coil in the gut of piglets.Moreover，it initially reveals the presence of a certain relationship between in feed copper and antibiotic resistance change of E.coil isolates form piglets.

This study was conducted to construct and select the efficient siRNA interference vector for porcine BPI gene，which provided the foundation for studying the function and mechanism of porcine BPI gene at the cellular level.Referring to the whole coding sequence of porcine BPI gene (GenBank accession number：EF436278)，porcine BPI specific hairpin siRNA fragments were designed and inserted into pcDNA 6.2-GW/EmGFPmiR vector.Four pairs of siRNA expression vectors (RB1，RB2，RB3，RB4) and one pair of negative control (NC) were constructed by PCR and sequencing verification.Then the above vectors were transfected into IPEC-J2 intestinal epithelial cell and further the interference effect was detected.The results showed that 4 BPI-specific siRNA vectors all reduced the BPI gene mRNA expression (P<0.05).Meanwhile the interference effect of RB4 vector was the best，whose efficiency reached 69%.This study successfully screened out the efficient siRNA vector which could exclusively interfere porcine BPI gene expression，which provided experimental basis for further studying the BPI gene function and mechanism for the resistance to gram-negative bacteria infection in porcine intestinal tract at the cellular level.

As a color-related gene，POMC plays an crucial role in the formation，development，pigmentation and other aspects of melanocytes.To study the expression level of POMC mRNA and localization of POMC protein in alpaca skin with different coat colors，quantitative real-time PCR (qRT-PCR) and immunohistochemistry were performed.The results showed that the quantity expression of POMC mRNA in brown alpaca was 23 times than in white alpaca (P＜0.01)，and POMC were expressed in root sheath and epidermis in both brown and white alpaca skin，moreover，the average protein expression of POMC in brown alpaca skin was higher than in white alpaca skin on the average optical density (P＜0.05).So POMC may be involved in the coat color formation.

 Haemophilus parasuis was one of the common bacterial disease pathogen of pigs，clinically characterized by fibrinous polyserositis，fibrinous arthritis and meningitis.It had importance significance that the expressed proteins of different virulent H.parasuis strains were profiled by the comparative proteomic approach to reveal the mechanism of pathogenisis.We chose 2 different virulent stains，stain A and stain B，of H.parasuis serotype 4 for comparative analysis，10 differently expressed proteins were analyzed and identified by 2-DE and MALDI-TOF-MS.In all proteins differentially expressed，3 protein spots were upregulated in strain A，they were glutamyl-tRNA synthetase，cysteine synthase and hypothetical protein，and 7 protein spots were upregulated in strain B included YaeT protein（omp85），CTP synthetase，aspartyl-tRNA synthetase（3 spots），uridylate kinase，protein-export chaperone SecB and so on.The function of expressed proteins mainly consisted of protein biosynthesis，amino-acid biosynthesis and pyrimidine biosynthesis.These results further enrich the information of virulence factors and pathogenic mechanisms of the H.parasuis strains，and also lay a foundation for study of immune proteomic.