MicroRNAs (miRNAs) are small noncoding RNA molecules that play important roles in the regulation of animal growth and development.Recent studies have demonstrated that miRNAs are required in the process of animal skeletal muscle development.Here，the recent advances of the roles of miRNAs in the skeletal muscle development were reviewed.

Avian eggs contain a variety of hormones of maternal origin.Glucocorticoids (GCs) are known to play critical roles in tissue differentiation，maturation and maternal programming in birds.In this paper，the role of glucocorticoids in embryonic development between human，mammals and avians are compared.The programming effects of glucocorticoids in avian and the research of possible epigenetic mechanisms are also reviewed and summarized.

As rate-limiting enzyme of regulating Nitric Oxide (NO) generation，endothelia nitric oxide synthase (eNOS) plays an important role to maintain the balance of NO，which is a vasodilatation factor.Life for a long time in high altitude hypoxic regions of Tibetan chicken，formed a unique mechanism of hypoxia adaptation.In this paper，the structure of eNOS gene，coding product features and functions were reviewed，meanwhile，eNOS gene of Tibetan chicken involved in genetic mechanism of hypoxia adaptation are discussed，in order to provide help for the researches of origin，evolution，breeding of poultry，etc.

 The objective of this study was to seek SNPs that influence Jinghai Yellow chicken’s ND and IB disease resistance character.The SNPs in Jinghai Yellow chicken were detected by SLAF-seq method and then the association of SNP sites with ND and IB disease resistance character were analyzed.The result showed that one SNP was significantly associated with ND disease resistance character，7 SNPs were potential significantly associated with ND disease resistance，and they were located in 5 genes：EEA1，CARS2，SCML2，GRP20 and TOMIL2；All of the 5 genes might influence or regulate organism’s disease resistance and immune ability and could be the candidate genes for Jinghai Yellow chicken’s disease resistance character.No SNP was found that was significantly associated with IB disease resistance character，this trait was complex and was rugulated by many factors.The results showed the 5 genes had influence on Jinghai Yellow chicken’s ND disease resistance characeter，and could provide the reference data for marker assisted selection of Jinghai Yellow chicken’s disease resistant breeding process.

The aim of this study was to investigate the effects of IGF-I and MSTN on goose skeletal muscle growth.In this study，the expression of IGF-I and MSTN in breast muscle of Taihu and Wanxi White goose at the age of 70 days were detected with Multiplex Competitive Fluorescent-PCR and Enzyme-linked immunoassay (ELISA) methods，and the correlations between gene expression levels and carcass traits were investigated.The result showed that the body weight and breast muscle weight of Taihu goose were significantly lower than those of Wanxi White goose at the age of 70 days，there was no significant gender difference in breast muscle percentage between the two breeds and there was significant gender difference in body weight and breast muscle weight between the two breeds.There was no breed and gender difference at the mRNA and protein levels of IGF-I and MSTN，and but in Taihu goose,IGF-I mRNA expression in male breast muscle was significantly higher than that of females.There was no significant correlation between mRNA expression and protein content of the two genes.There was a positive correlation between IGF-I and MSTN at the protein level of breast muscle.There was a negative correlation between IGF-I mRNA expression and body weight，breast muscle weight and breast muscle percentage in goose，and a negative correlation between the expression of MSTN and body weight had been detected in Wanxi White goose.The results suggested that the expression levels of genes involving in positive and negative regulation to muscle growth may be in a dynamic balance，special participated in the growth regulation on Wanxi White goose at the age of 70 days.

The study was conducted to clone and analyze CDS sequence of pig Fosl2 gene，and to investigate the developmental expression of Fosl2 in pig various tissues(heart，liver，spleen，lung，kidney，the longissimus muscle and backfat) at different stages.In this study，Yunan Black pig was used as experimental animal to clone the CDS region of Fosl2 gene by RT-PCR，the protein structure were predicted by bioinformatics，meanwhile，qRT-PCR was used to analyze developmental expression of Fosl2 gene in pig from 7- to 180-day-old.The results showed that the CDS of pig Fosl2 gene was 984 bp，encoding 327 amino acids.It had no signal peptide in peptide chain，but had a transmembrane domain and a basic-domain leucine-zipper，which were from 17 to 33 amino acids and from 122 to 187 amino acids,respectively.The protein of pig Fosl2 had high conservatism among mammal，and it was transmembrane and non-secretory protein.The Fosl2 was widely expressed in tissues，the expression of Fosl2 in lung was higher than other tissues at the same stage，it was deduced that pig Fosl2 might be related to the process of lung development；the variation trend in backfat showed that Fosl2 might had the function enhancing adipogenesis and then regulate lean meat percentage.Therefore，the results of this study may lay foundation for further research on the structure and the role of Fosl2 gene in physiogenesis in pig.

The objectives of this study were to analyze the goat beta-defensins 104a (gBD104a) gene by bioinformatics，and to investigate the expression levels of gBD104a in various tissues of adult bucks and the location in reproduction organ and sperms.Bioinformatics analysis of gBD104a (GenBank No.：KJ508074.1) was made by software online.Relative mRNA expressions were detected by real-time fluorescent quantitative PCR.Cellular localization of gBD104a in testes，epididymidis and sperms were examined by immunohistochemistry.The results showed that：(1) The results of bioinformatics analysis showed that the cDNA of gBD104a gene contains 324 nucleotides，including a complete open reading frame (ORF) encoding 107 amino acids，software online predictded 1-19 amino acids as signal peptide area，27-55 amino acids for potential beta defensins motif，7 potential O-glycosylation sites on C-terminal；(2) The results of QRT-PCR showed that gBD104a mRNA was widely expressed，but the relative expression of gBD104a mRNA was the highest in cauda epididymis.Expressions of gBD104a mRNA in caput epididymis，tracheal，abomasums，jejunum and ileum were more than those in other tissues；(3)The results of immunohistochemistry showed that there were strong positive signal of gBD104a in pseudostratified ciliated columnar epithelium of caput and corpus，and strong positive signal in columnar epithelium of cauda.gBD104a was located on the sperm acrosome and mitochondria surface.gBD104a was an epididymal secretory glycoprotein.gBD104a mRNA was widely expressed in various tissues of bucks，while the highest expression levels was in cauda epididymis.gBD104a was coated on sperm surface，which maybe play an important role in sperm motility，acrosome reaction and capacitation process.Therefore，the physiological functions of gBD104a needs be further researched.

To further understand the function of Fas/ FasL system，the Fas and FasL genes of yak were cloned by RT-PCR method and their expressions were analyzed by combination of RT-PCR and immunohistochemistry.A complete cDNA sequences in 2 605 bp of Fas gene and 1 572 bp of FasL gene of yak was obtained.These sequences were highly homology with cattle.Fas and FasL mRNAs were co-expressed in all analyzed tissues except heart which expressed Fas mRNA alone.The two genes strongly co-expressed in liver，spleen，kidney，lymphonodus，thymus and testis.A cytoplasmic form of FasL protein was detected in several cell types of various organs.Fas and FasL were strongly co-expressed in lymphoid organs and immune privilege sites，they were also strongly co-expressed in other tissues like kidney and liver.The results suggested that Fas/FasL system was more complicated.

To better understand the role of melatonin receptor (MT1) during pregnancy of yak，the expression level of MT1 protein and MT1 mRNA in uterus of yak (Bos grunniens)during pregnant and nonpregnant stage was determined using Streptavidin-Peroxidase (S-P) immunohistochemical method and real-time PCR，respectively.The results showed that MT1 protein expressed in stromal cells and myometrial smooth muscle cells during pregnancy and nonpregnant stage (P>0.05).The expression level of MT1 protein in endometrial blood vessel and vascular smooth muscle had no significant difference on 28-35 day of pregnancy compared with that of non-pregnant yak，however，it was increasing with the stage of pregnancy (P<0.05).Meanwhile，MT1 protein expression was not significant different on 28-35 day of pregnancy in gland epithelium，but had dramatic increases on 50-60 day of pregnancy (P<0.05) and 90-100 day of pregnancy (P<0.01).In surface epithelium cells，the expression of MT1 protein was lower on 28-35 day of pregnancy than that of nonpregnant stage (P<0.05) and had significant decreases on 50-60 day of pregnancy (P<0.01) and 90-100 day of pregnancy (P<0.01).In addition，MT1 mRNA expression was lower in pregnant uterus than that of nonpregnant stage (P<0.01).The results indicated that the decreased MT1 mRNA expression in uterus during pregnancy might be associated with gland epithelium cell function for maintenance of pregnancy.

This study was performed to investigate the expression of cell cycle regulation genes CyclinB1 and p34^cdc2 in testis of calves at different ages.The eighteen 5-6 days healthy Holstein calves (with similar weight) were randomly divided into 3 groups and fed with the similar basal diet.The testes of 2 calves from each group were collected at the time of 30 ，60 and 90 d respectively.QRT-PCR method was used to detect the mRNA expression of the cell cycle regulatory genes CyclinB1 and p34^cdc2 in testes of calves at different ages.The location of CyclinB1 and p34^cdc2 in the testes was detected by immunohistochemical methods.The results showed that the highest expression of CyclinB1 and p34^cdc2 mRNA and protein was obtained in the calves’ testes of 90 d.The mRNA and protein expression levels of CyclinB1 and p34^cdc2 increased with the increasing age.The mRNA expression level of the 2 genes at the age of 90 d were significantly higher than that at 30 and 60 d (P<0.05).However，no significant difference was observed between 30 and 60 d (P>0.05).The protein expression of p34^cdc2 in the testes of calves at different ages was significant (P<0.05).Although the protein expression of CyclinB1 at 60 and 90 d had no significant difference(P>0.05)，they were significantly higher than those at 30 d.This study indicated that the expression of CyclinB1 and p34^cdc2  mRNA and protein increased with the increasing age and CyclinB1 and p34^cdc2 in the testes of calves at different ages was significant.

In this study，the Zhongnuo No.1，Zhongnuo No.2，and Jingkenuo 2000 were used as experimental materials to investigate the influence of harvesting time on the nutritional components and DMD of waxy corn stalks after earing.The results showed that with the extension of time after stripping，the contents of NDF and ADF of the waxy corn stalks decreased，the content of WSC increased，and DMD increased.Moreover，the content of total sugars of the waxy corn stalks increased and the content of ADL decreased.Obviously，the nutritive value of waxy corn stalks increased with the extension of time after earing.

To identify the pathogenic characteristics of mouse-adapted Scrapie Strain RML propagated in N2a cell line，we intracerebrally inoculated mice with the lysate of Sc-N2a cell culture.After 200±28 days’ incubation period，all inoculated C57BL/6J mice appeared typical ataxia symptoms of TSEs.The PrP^Sc from both brain tissues and Sc-N2a cells shared the same biochemical aspects.Western blotting result confirmed that they had the same glycosylation patterns，the same size distribution of PK digestion fragments，with higher levels of monoglycosylated.The pathological changes of brain sections from inoculated mice at the terminal stage were analyzed by HE stain and IHC assay.Vacuoles were mainly found in brain stem，cortex，cerebellum and thalamus，while PrP^Sc deposits mainly in cortex，hippocampus，thalamus，cerebellum.The results of mouse regression test showed that the PrP^Sc propagated in N2a cell line had pathogenicity，and kept the the stain characteristics of mouse-adapted scrapie strain RML.The Sc-N2a cell line is an ideal model for the study of TSEs.

The chicken infectious bronchitis (IB) is a broad epidemic and infectious disease，and became a critical threat to poultry industry in China.To understand the current national IBV epidemiological situation，3 132 samples collected between 2010 and 2012 in China have been tested by the Ringpu Diagnosis Research and Service Center.There were 410 IBV positive results by RT-PCR method，then statistic and analyzed by cross-references，such as by time，geography，clinical symptoms and animal species etc.The results showed that 1) 13.1% of all samples were IBV positive，and 26.7% of positives were multiplex infection，especially with AIV-H9 or NDV.2) White feather broilers are more susceptible to the wild IBV，the isolation rate was 13.6%.3) The IBV incidence showed quite clear seasonal changes，especially between November and next May.4) The main clinical symptoms of IBV were kidney swelling and respiratory symptoms，also showed egg drop and proventriculitis sometimes.5) The 3-5 weeks old white feather broilers had the highest IBV isolation rate，but also the 3-5 and 25-30 weeks old period of layer chicken.Through the epidemiological investigation on IBV，it provides important scientific data and evidences which are important to develop effective strategies for prevention and controlling IB.

One strain of the inclusion body hepatitis virus (IBHV) was isolated and identified based on pathological changes，PCR and sequence analysis in sick chicken.The DNA of the IBHV was extracted from allantoic fluid obtained from SPF egg，which was inoculated with the sick chicken liver.The primers were designed based on the conserved hexon sequence of avian adenovirus.The PCR product of avian adenovirus was amplified and sequenced.The fatty degeneration，karyopyknosis，cell necrosis and basophilic inclusion bodies were observed in liver tissue from sick chicken.The sequencing results indicated that the virus shared 98.6% identity with FAV-4 in group Ⅰ and 68.7%-96.9% identity with other serotypes in group Ⅰ，and 44.6% identity with hemorrhagic enteritis virus in group Ⅱ and egg drop syndrom virus in group Ⅲ.These results confirmed that the isolated virus is a member of fowl adenovirus groups，and can cause chicken inclusion body hepatitis.Our research provides the basis for isolation and identification of group Ⅰ avian adenovirus.

To explore the feasibility of attenuated Salmonella typhimurium as the carrier of porcine epidemic diarrhea virus (PEDV) S gene，the biological characteristics of three recombinant attenuated Salmonella strains harboring varying length of PEDV S gene were researched.The biological characteristics research of SL7207(pVAX-S_499-650) (456 bp，encoding 499-650 amino acid of N-termanal S protein，SL7207(pVAX- S_499-789) (873 bp，encoding 499-789 amino acid of N-termanal S protein)，SL7207(pVAXD-S_1-789) (2 367 bp，encoding 1-789 amino acid of N-termanal S protein) which harboring PEDV S genes in varying length plasmid DNA showed：SL7207(pVAX-S_499-650)，SL7207(pVAX-S_499-789) and SL7207(pVAXD-S_1-789) were steady in vivo/vitro；the target genes could be transcribed in the ileum tissue of mouse；recombinant strains could colonize in liver and spleen of mice and gradually eliminated by host；safety to BALB/c mouse at the dosage of 1×10⁹ CFU by oral administration.All of this，demonstrating the attenuated S.typhimurium could be the carrier of PEDV S gene and laying the foundation for evaluating the immunogenicity of recombinant attenuated Salmonella strains in piglet.

This study aimed at analyzing the molecular characterization of the tuf gene in Mycoplasma ovipneumoniae.The tuf genes of the reference Y98 strain and 11 clinical isolates were cloned and sequenced，and then were used for bioinformatics analysis.A phylogenetic tree was constructed base on the tuf genes.The results showed that the open-reading frames (ORFs) of tuf genes in all the strains were 1 209 bp，which encoded 402 amino acids with 93.3%-100% amino acid identity and possessed higher antigenic index and higher lymphocyte epitopes.The average GC content of the protein coded by tuf gene was 39.80%.The Tuf proteins of reference Y98 strain and 10 clinical isolates contained one strong transmembrane region and were absence of signal peptide，and contained six different functional sites.While another isolate contained two transmembrane regions，with a additional N-glycosylation site in the site of 271-274.The genes from 12 strains were identified 117 single nucleotide polymorphisms (SNPs)，consisting of 41 synonymous polymorphisms and 76 non-synonymous polymorphisms leading to 53 amino acid changes，mainly in 325-354 region near the carboxyl region，which maybe leading to change the function of this protein.The phylogenetic relationship of M.ovipneumoniae based on whole genome and that based on the tuf gene were consistent.So，the tuf gene is a better target than 16S rRNA gene for the phylogenetic analysis for M.ovipneumoniae.Despite of its conservation，the tuf gene still showed genetic diversity within the strains，making it potential for molecular typing.

In order to determine the cell adhesion and invasion characteristics of Gallibacterium anatis（G.anatis），an in vitro HeLa cell model was established.The adhesion and invasion characteristics of 2 strains were evaluated by means of adhesion and invasion experiment，Gram and Giemsa staining，Scanning electron microscopy and Transmission electron microscopy.Adhesion counting assay demonstrated that the high adhesive of G.anatis Yu-PDS-RZ-1-SLG strain and adhering bacteria numbers were 0.84•cell^－1，4.68•cell^－1，8.52•cell^－1，9.27•cell^－1 and 8.2 •cell^－1 at 30，60，90，120，150 min after infection，respectively.G.anatis F149^T strain revealed a weak adhesive level.Invasion assay showed weak invasive of G.anatis Yu-PDS-RZ-1-SLG strain and no invasion of G.anatis F149^T strain，and invading bacteria numbers of G.anatis Yu-PDS-RZ-1-SLG strain reached the maximum at 150 min，about 8.27 per 1 000 cells，but cell disruption，cell activity weakened subsequently.Staining results and electron microscopy experiments further confirmed that G.anatis Yu-PDS-RZ-1-SLG strain adhere to surface and invade Hela cells.Adherence was prevented by treating bacterial cells with trypsin，suggesting the participation of proteins in this process.The adhesive and invasive capacity could be an important ability for colonization of tissue surfaces of G.anatis.The establishment of cell model provided the basis for further research on pathogenic mechanism of G.anatis.

The aim of the present study was to investigate the expression of PGP9.5 and NPY in the normal testis and cryptorchidism of Bactrian camel.The cryptorchidism and the normal testis of bactrian camel were studied by immunohistochemical SP technique and IPP (Image-Pro Plus) statistics method.The results showed that the PGP9.5 immunoreactive products distributed strongly in the Sertoli cells，sperm cells，arteries and veins of the normal testis，as well as NPY was moderate positive in Sertoli cell，sperm cells and arteries but weak positive in veins，in addition，Leydig cells in adult camels were strongly immunostained with NPY not only in normal testis but also in cryptorchidism.However，immunoreactivity of PGP9.5 and NPY were detected in cryptorchidism testis with significant differences intensities compared with the normal.These findings suggest that PGP9.5 and NPY are detected in the majority of cells in the seminiferous tubules and the blood vessels are involved in spermatogenesis and secretion of testosterone in Bactrian camel testis，they may play important roles in Bactrian camel male fertility and infertility.

The present study was aimed at the effects of antibiotics on LPS induced liver cell injury and hepatoprotective activity of compound ammonium glycyrrhizin （CAG）.Chicken hepatocytes were isolated by semi-situ perfusion method.After the cells were cultured for 48 h，LPS was added with different concentrations to screen the optimal dose of liver cell injury.At the half of the optimal dose of LPS，enrofloxacin，florfenicol and amoxicillin were added with different concentration to detect the indexes of liver cell injury.Hepatocytes were treated with different concentrations of CAG for 24 h after the addition of LPS （30 μg•mL^-1） + enrofloxacin （80 μg•mL^-1）.Cell viability and activities of ALT，AST，SOD，GSH，MDA were measured，flow cytometric analysis was conducted to detect the early apoptosis ratio of hepatocytes.The result showed that when LPS less than 50 μg•mL^-1，the liver injury was not obvious.While LPS at more than 60 μg•mL^-1，the cell viability were significantly lower and the activities of ALT，AST in the culture media were significantly higher than those of controls（P＜0.01）.LPS （30 μg•mL^-1） combined with enrofloxacin （80 μg•mL^-1），florfenicol （40 μg•mL^-1），amoxicillin （60 μg•mL^-1） could induce liver cell injury significantly.CAG （25，50，100，200，400 μg•mL^-1） could markedly reduce the numbers of early apoptotic cells，inhibit the elevation of ALT，AST，MDA，and significantly increase the reduced level of SOD，GSH and cell viability.These results indicated that LPS could induce liver cell injury.Antibiotics might contribute to the liver cell injury induced by LPS.CAG counteracts liver cell injury at various levels by preventing apoptosis and oxidative stress damage.

In order to investigate the effects of antagonist for the Combined Anaesthetic for Miniature Pigs (XFM) on p-p38 protein and c-myc gene in different encephalic region of rats，and discuss the mechanism of XFM antagonist.Eighteen Wistar rats were divided randomly into C (Control group) and J (Antagonist group) group.J group consisted of two subgroups，J1(5 min after rats received the XFM antagonist intraperitoneally) and J2 (1 h after rats received the XFM antagonist intraperitoneally).The brain tissues were collected at each time point.The expression of p-p38 in different brains regions were detected by Western blot.The transcription of c-myc mRNA in different brains regions were detected by real time PCR.The results showed that the expression of p-p38 and c-myc mRNA in cerebral cortex，thalamus and brain stem increased significantly；the expression of p-p38 and c-myc mRNA in cerebellum and hippocampus decreased significantly.These results indicated that p-p38 protein and c-myc gene were affected by the XFM antagonist，this may be one of the antagonism mechanisms.

 The aim of this study was to systematically elucidate the mechanism of heat stress-induced swine immunosuppression，and inflammatory factors disorder.The TZ (Luchuan pig×Landrace pig)porcine SOCS4 gene was cloned in this study，it consisted of 1 326 bp of ORF encoding a protein with 441 amino acids，285 amino acids of them formed the N-terminal of SOCS4.It shared high amino acid sequence identity with that in Landrace pigs(100.0%)，Bos Taurus(96.1%)，Capra Hircus(96.1%)，Felis catus (95.2%)，Equus caballus(94.6%)，Canis lupus familiaris (94.3%)，Homo sapiens  (93.0%) and Mus musculus (88.1%).The molecular weight of SOCS4 protein was 50.5 ku，and PI was 6.60.qRT-PCR results showed that the expression of SOCS4 in thymus gland of TZ pigs was significantly upregulated on day 3 and 9 after heat stress began (P≤0.05).As well in mesenteric lymph node，where it significantly upregulated on day 1 and 6 (P≤0.01).The expression of SOCS4 decreased  significantly in small intestine and spleen of TZ pigs during heat stress (P≤0.05).The TZ porcine SOCS4 cDNA was cloned successfully，and a significant changes of SOCS4 mRNA expression were appeared in heat stressed pigs with a tissue and time dependence manner.

This study aimed to investigate the effects of vitamin E added in diet on sperm DNA integrity and antioxidant activities of semen in mice.50 mice divided into 5 groups were fed in the experiment，in which vitamin E was added in diet with the different content of 0（Control），4，8，12 and 16 g•kg^-1，respectively.The mice were killed by cervical vertebra dislocation and dissected after 30 days when semen was obtained from epididymis and seminiferous tubules.The sperm motility，density，deformity rate and DNA integrity were detected by single cell gel electrophoresis(SCGE) assay and test kits，as well as the antioxidant activities including superoxide dismutase (SOD)，glutathione peroxidase (GSH)，glutathione reductase (GR) and catalase (CAT).The percentage of “grade 0” sperm in diet supplemented with 12 g•kg^-1 vitamin E was significantly higher than that of other treatment groups (P<0.05).The sperm motility was significantly improved (P<0.05) and the levels of GR and SOD in seminal plasma were significantly higher (P<0.05) by the supplementing with 8-16 g•kg^-1 vitamin E in diet than the control，respectively.The CAT activity in seminal plasma reached the highest level with supplementation of 12-16 g•kg^-1 vitamin E in diet，when compared to the others (P<0.05).In conclusion，we could infer that 12 g•kg^-1 vitamin E added could obviously improve the sperm DNA integrity and the antioxidant activities of CAT，GR，GSH and SOD in seminal plasma.

Salmonella onarimon is a rare serotypes in Salmonella，and its pathogenicity has not been reported.The aim of this experiment was to study the pathogenicity of S.onarimon strain swun3736 isolated from yak in BALB/c mice.Using oral method，we studied its pathogenicity by the median lethal dose (LD₅₀)，the dynamic distribution and histopathological changes in tissues of mice.The results showed that the LD₅₀ was 5 × 10² CFU.When infected with 10⁵ CFU per mice，bacteria were first isolated from lungs at 24 h PI，and spleen，liver，kidneys and pancreas at 72 h post infection (PI)，while brain and heart at 96 h PI.In histopathological examination，it appeared the degeneration，necrosis，inflammatory cell infiltration and other serious damage in the lung，spleen，liver，heart，kidney and ileum.While excepting for ileum，other intestinal bowels exhibited no obvious lesions，suggesting that the ileum was the invasion portal of swun3736.These results demonstrated that S.onarimon swun3736 has strongly virulence，which indicated that public hygienics significance was worth concerned.

From Aug 2013 to Mar 2014，fattened goat herds of many farms in Jiangsu province suffered severe respiratory disease.Serum and nasal swab samples were collected and subjected to pathogen detection.Parainfluenza virus type 3 (PIV3) was detected in most of the samples.Positive samples were inoculated onto Madin-Darby bovine kidney (MDBK) cells and passaged 5 times for virus isolation.A novel strain，named JS2013，was successfully isolated as identified by RT-PCR and hemagglutination test.Entire M coding region was amplified and sequenced.Phylogenetic analysis and comparison to other reference sequences available in GenBank indicated JS2013 was clearly distinct from other HPIV3 and BPIV3 strains.This is the first isolation and partial genetic identification of PIV3 from diseased goats.