IGF1R和IGF2R在南江黄羊不同组织和肌细胞中的表达模式比较

doi:10.11843/j.issn.0366-6964.2019.04.003

Abstract

Abstract:

To investigate the mRNA expression patterns of IGF1R and IGF2R genes in different tissues and muscle cells of goats, real-time quantitative PCR was used to detect the mRNA levels of IGF1R and IGF2R in longissimus dorsi muscle at 3 prenatal stages (E45, E60 and E105), in muscle tissues (longissimus dorsi muscle, triceps brachii muscle) and visceral tissues (heart, liver and lung) at 5 postnatal stages (B3, B30, B60, B90 and B120) of Nanjiang Brown goats. Moreover, the expression patterns of IGF1R and IGF2R during skeletal muscle satellite cells (SMSCs) proliferation and differentiation was determined. The results showed that, in longissimus dorsi muscle of prenatal goats, the expression of IGF1R and IGF2R increased, then decreased. And the IGF1R expression level was higher than IGF2R in E45 and E60 (P<0.05, P>0.05), but lower than IGF2R in E105 (P<0.01). At the postnatal stages, the expression level of IGF1R was the highest in lung, and the lowest in liver; In contrast, the expression level of IGF2R was the lowest in lung but the highest in longissimus dorsi muscle. With the development of lambs, IGF1R and IGF2R exhibited ascending expression in heart, fluctuated (down-up-down) in liver, while the trends of IGF1R and IGF2R expression was reverse in lung (before B90). Furthermore, in longissimus dorsi and triceps brachii muscle, IGF1R showed increasing expression tendency(before B90), while IGF2R showed a down-up-down trend. Furthermore, IGF1R and IGF2R showed a up-down expression pattern during the proliferation stages (24, 48 and 72 h) of SMSCs, but was low at the early differentiation stages from 0 to 3 day, followed by significantly increased at 5 day (P<0.05), and then decreased. Expression patterns of IGF1R and IGF2R genes in different tissues and SMSCs of Nanjiang Brown goats were presented in this study. It is preliminarily reveal that high expression of IGF1R in lung and low expression in liver play a key role in organ growth and function maintenance, IGF1R synergizes with IGF2R to promote muscle tissue development and muscle cell proliferation and differentiation.

Key words: Nanjiang Brown goat, IGF1R, IGF2R, tissues, satellite cells, gene expression

CLC Number:

S827.2

ZHAN Siyuan, DING Xue, ZHONG Tao, WANG Linjie, LI Li, ZHANG Hongping. Comparison of IGF1R and IGF2R Expression Patterns in Different Tissues and Muscle Cells of Nanjiang Brown Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 701-711.

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Comparison of IGF1R and IGF2R Expression Patterns in Different Tissues and Muscle Cells of Nanjiang Brown Goats

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