云南部分地区猫血巴尔通氏体感染状况的监测

doi:10.11843/j.issn.0366-6964.2018.11.026

Abstract

Abstract:

The present study was to examine the prevalence of Haemobartonella felis infections in cats from some areas of Yunnan province, a total of 338 fresh blood were collected and was detected using PCR amplification of the 16S rRNA gene, and the product of PCR were sequenced and compared the phylogenetic analysis. Results showed that 21 samples were positive, and the overall prevalence of H. felis was 6.21% (21/338). Twenty samples were infected with Candidatus Mycoplasma haemominutum, and one sample was infected with Mycoplasma haemofelis. Analysis of influences factors showed that there was a significant difference between breeding mode and age (P<0.05). The present investigation firstly report of the H. felis infection in cat in Yunnan and the study provide the basic data for preventing and controlling H. felis infection in cats from Yunnan Province.

Key words: Haemobartonella felis, 16S rRNA, epidemiological survey

CLC Number:

S852.72

Lü Yan, ZHAI Guo-lian, CHANG Jiang-yan, SHI Lian-qin, YUAN Zheng-ju, ZHAO Huan-yun, ZOU Feng-cai, DUAN Bo-fang. Surveillance of Cat Haemobartonella felis in Some Areas of Yunnan Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2529-2534.

References

[1] HARVEY J W, GASKIN J M. Experimental feline haemobartonellosis[J]. J Am Anim Hosp Assoc, 1977, 13(1):28-38.
[2] MESSICK J B. Hemotrophic mycoplasmas (hemoplasmas):a review and new insights into pathogenic potential[J]. Vet Clin Pathol, 2004, 33(1):2-13.
[3] FLINT J C, MOSS L C. Infectious anemia in cats[J]. J Am Vet Med Assoc, 1953, 122(910):45-48.
[4] 庄庆均,张浩吉,白挨泉,等. 我国猫Candidatus Mycoplasma haemominutum的分子鉴定及其系统发生关系[J]. 中国兽医科学, 2010, 40(6):551-555.
ZHUANG Q J, ZHANG H J, BAI A Q, et al. Molecular identification and phylogenetic relationship of Candidatus Mycoplasma haemominutum from cat in China[J]. Chinese Veterinary Science, 2010, 40(6):551-555. (in Chinese)
[5] SPLITTER E, CASTRO E, KAN AWYER W. Feline infectious anemia[J]. Vet Med, 1956, 51:17-22.
[6] WILLI B, BORETTI F S, CATTORI V, et al. Identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland[J]. J Clin Microbiol, 2005, 43(6):2581-2585.
[7] TASKER S, PETERS I R, PAPASOULIOTIS K, et al. Description of outcomes of experimental infection with feline haemoplasmas:copy numbers, haematology, Coombs' testing and blood glucose concentrations[J]. Vet Microbiol, 2009, 139(3-4):323-332.
[8] PETERS I R, HELPS C R, WILLI B, et al. The prevalence of three species of feline haemoplasmas in samples submitted to a diagnostics service as determined by three novel real-time duplex PCR assays[J]. Vet Microbiol, 2008, 126(1-3):142-150.
[9] JENSEN W A, LAPPIN M R, KAMKAR S, et al. Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats[J]. Am J Vet Res, 2001, 62(4):604-608.
[10] AQUINO L C, HICKS C A E, SCALON M C, et al. Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species[J]. J Microbiol Methods, 2014, 107:189-196.
[11] TASKER S, BINNS S H, DAYM J, et al. Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in cats in the United Kingdom[J]. Vet Rec, 2003, 152(7):193-198.
[12] TASKER S, BRADDOCK J A, BARAL R, et al. Diagnosis of feline haemoplasma infection in Australian cats using a real-time PCR assay[J]. J Feline Med Surg, 2004, 6(6):345-354.
[13] FOLEY J E, HARRUS S, POLAND A, et al. Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats[J]. Am J Vet Res, 1998, 59(12):1581-1588.

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Surveillance of Cat Haemobartonella felis in Some Areas of Yunnan Province

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