植物性神经对围着床期大鼠子宫中肥大细胞和组织胺含量的影响

doi:10.11843/j.issn.0366-6964.2018.11.012

Abstract

Abstract:

The objective of this study was to explore the relationship between autonomic nerve and cellular immune in uterus during peri-implantation based on mast cell (MC) count and histamine content. Ninety healthy female Wistar rats weighed 300-350 g were randomly assigned into 3 groups. One group as neurectomy (the autonomic nerves governing the uterus from the pelvis nerve and mesenteric caudal ganglion were amputated by surgical operation before pregnancy) experiment group, one group as sham operation group and the other as the control. After fully recovering from the surgery, female rat was cohabited with male rat in ovulatory period. On day 4, 5, 6 of pregnancy(d4, d5, d6), the females were killed by neck dislocation, uteri and ovary were surgically removed in abacterial condition. The number of ovulation sites was observed under an anatomy microscope and the quantity of embryos was counted, MC count was determined by cell staining, and histamine was assayed using histamine ELISA kits. The results showed that the spontaneous release and content of histamine in the uterus of normal rats were the lowest level at the time of implantation (d5). The MC count presented the similar changes to histamine. Compared with the control, spontaneous release of histamine from uterus in operated rat showed a significant decrease at d4, d6 of pregnancy (P<0.05), amputation of the nerve innerving uterus very significantly decreased the total content of histamine (P<0.01) before embryo implantation (d4), meanwhile the number of implanted embryo was also markedly decreased or delay. There were similar changes in the number of uterine MC and histamine content during implantation after neurectomy. These results suggested that resection of the nerve innerving uterus decreased MC counts and histamine level in the period of pre-implantation, changed uterine local immunity level, leading to reduction or delay of implantation. Taken together, our results imply that the autonomic nerve innerving uterus can up-regulate the local immune level in the the uterus through the proliferation of MC and histamine releasing, and play an important regulating role in embryo implantation.

Key words: rats, uterus, autonomic nerve, mast cell, histamine

CLC Number:

Q425

HUANG Li-bo, ZHANG Xiu-xia, FAN Xiao-jie, HOU Yan-meng, JIANG Shu-zhen, LU Xuan, YUAN Xue-jun. Effects of Autonomic Nerve on Mast Cell and Histamine Content in Rat Uterus during Peri-implantation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2409-2415.

References

[1] KRYSTEL-WHITTEMORE M, DILEEPAN K N, WOOD J G. Mast cell:a multi-functional master cell[J].Front Immunol,2016,6:620-630.
[2] MOON T C, BEFUS A D, KULKA M. Mast cell mediators:their differential release and the secretory pathways involved[J].Front Immunol,2014,5:569-586.
[3] RUDOLPH M I, ROJAS I G, PENISSI A B. Uterine mast cells:a new hypothesis to understand how we are born[J]. Biocell, 2004,28(1):1-11.
[4] JEZIORSKA M, SALAMONSEN L A, WOOLLEY D E. Mast cell and eosinophil distribution and activation in human endometrium throughout the menstrual cycle[J].Biol Reprod,1995,53(2):312-320.
[5] DENG Z P, LIU Y W, ZHOU Z X. Quantitative studies of mast cells and histamine in the endometrium of cow and sheep[J].China Vet Sci,1994,1:50-55.
[6] VAN MOURIK M S, MACKLON N S, HEIJNEN C J. Embryonic implantation:cytokines, adhesion molecules, and immune cells in establishing an implantation environment[J].J Leukoc Biol,2009,85(1):4-19.
[7] ZHAO X M, MA W G, DAS S K, et al. Blastocyst H₂ receptor is the target for uterine histamine in implantation in the mouse[J].Development,2000,127(12):2643-2651.
[8] COCCHIARA R, DI TRAPANI G, AZZOLINA A, et al. Early embryonic histamine-releasing factor:a new model for human implantation[J].Hum Reprod,1986,1(7):445-448.
[9] PYZLAK M,SZEWCZYK G,SZUKIEWICZ D, et al. Histamine influence on apoptosis in trophoblast cell cultures[J].Inflamm Res,2010,59(Suppl 2):213-215.
[10] LIU Z T, KILBURN B A, LEACH R E, et al. Histamine enhances cytotrophoblast invasion by inducing intracellular calcium transients through the histamine type-1 receptor[J].Mol Reprod Dev,2004,68(3):345-353.
[11] SZEWCZYK G, SZUKIEWICZ D, KLIMKIEWICZ J, et al. Influence of histamine on the process of human trophoblast differentiation[J].Inflamm Res,2005,54(Suppl 1):S78-S79.
[12] LEBER A, ZENCLUSSEN M L, TELES A, et al. Pregnancy:tolerance and suppression of immune responses[M]//CUTURI M C, ANEGON I. Suppression and Regulation of Immune Responses:Methods and Protocols.Totowa, NJ:Humana Press,2011:397-417.
[13] PARIA B C, DAS N, DAS S K, et al. Histidine decarboxylase gene in the mouse uterus is regulated by progesterone and correlates with uterine differentiation for blastocyst implantation[J]. Endocrinology, 1998,139(9):3958-3966.
[14] YUAN X J, HUANG L B, QIAO H L, et al. Uterine autonomic nerve innervation plays a crucial role in regulating rat uterine mast cell functions during embryo implantation[J]. Prostaglandins Other Lipid Mediat,2009,90(3-4):94-97.
[15] DA SILVA E Z M, JAMUR M C, OLIVER C. Mast cell function:a new vision of an old cell[J].J Histochem Cytochem, 2014,62(10):698-738.
[16] CARDAMONE C, PARENTE R, DE FEO G, et al. Mast cells as effector cells of innate immunity and regulators of adaptive immunity[J].Immunol Lett,2016,178:10-14.
[17] FROSSI B, GRI G, TRIPODO C, et al. Exploring a regulatory role for mast cells:‘MCregs’?[J].Trends Immunol,2010,31(3):97-102.
[18] SUTO H, NAKAE S, KAKURAI M, et al. Mast cell-associated TNF promotes dendritic cell migration[J]. J Immunol,2006,176(7):4102-4112.
[19] DRUBE S, KRAFT F, DUDECK J, et al. MK2/3 are pivotal for IL-33-induced and mast cell-dependent leukocyte recruitment and the resulting skin inflammation[J]. J Immunol,2016,197(9):3662-3668.
[20] POPOVIC M, TELES A, STANCIC O, et al. Mast-cell-associated genes Thp1, Mcpt1 and Mcpt5 are up-regulated after Treg-induced tolerance at the fetal-maternal interface:new role for mast cells in tolerance?[J]. J Reprod Immunol,2007,75(1):A16-A16.
[21] SALAMONSEN L A, JEZIORSKA M, NEWLANDS G F, et al. Evidence against a significant role for mast cells in blastocyst implantation in the rat and mouse[J]. Reprod Fertil Dev,1996,8(8):1157-1164.
[22] MENZIES F M, HIGGINS C A, SHEPHERD M C, et al. Mast cells reside in myometrium and cervix, but are dispensable in mice for successful pregnancy and labor[J]. Immunol Cell Biol,2012,90(3):321-329.
[23] WOIDACKI K, POPOVIC M, METZ M, et al. Mast cells rescue implantation defects caused by c-kit deficiency[J]. Cell Death Dis,2013,4:e462-e472.
[24] WOIDACKI K, JENSEN F, ZENCLUSSEN A C. Mast cells as novel mediators of reproductive processes[J]. Front Immunol,2013,4:29-35.
[25] KABASHIMA K, NAKASHIMA C, NONOMURA Y, et al. Biomarkers for evaluation of mast cell and basophil activation[J].Immunol Rev,2018,282(1):114-120.
[26] PADILLA L,REINICKE K,MONTESINO H, et al. Histamine content and mast cells distribution in mouse uterus:the effect of sexual hormones, gestation and labor[J].Cell Mol Biol,1990,36(1):93-100.
[27] 袁学军,乔惠理. 支配子宫的植物性神经对子宫免疫的影响I.切断支配子宫的植物性神经对SD大鼠子宫肥大细胞的影响[J]. 中国兽医学报, 2000, 20(3):290-292.
YUAN X J, QIAO H L. Effect of cutting autonomic nerves controlling uterus on the rat mast cells in uterus[J]. Chinese Journal of Veterinary Science, 2000,20(3):290-292.(in Chinese)
[28] BUHNER S, BARKI N,GREITER W, et al. Calcium imaging of nerve-mast cell signaling in the human intestine[J].Front Physiol,2017,8:971-982.
[29] FORSYTHE P. The parasympathetic nervous system as a regulator of mast cell function[M].HUGHES M R, MCNAGNY K M. Mast Cells:Methods and Protocols. New York, NY:Humana Press,2015:141-154.
[30] ZHU T H,DING S J,LI T T, et al. Estrogen is an important mediator of mast cell activation in ovarian endometriomas[J].Reproduction,2018,155(1):73-83.

Effects of Autonomic Nerve on Mast Cell and Histamine Content in Rat Uterus during Peri-implantation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HU Yanqing, XU Kui, WEI Yinghui, QIU Yiqing, ZHANG Xiuling, WANG Bingyuan, LIU Zhiguo, MU Yulian, LI Kui. The Effect of PNPLA5 Knockout on the Testis Morphology and Sperm Motility in Rats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 71-77.
[2]	HU Yanqing, XU Kui, WEI Yinghui, QIU Yiqing, ZHANG Xiuling, WANG Bingyuan, LIU Zhiguo, MU Yulian, LI Kui. The Effect of PNPLA5 Knockout on the Testis Morphology and Sperm Motility in Rats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 71-77.
[3]	HUANG Li-bo, ZHANG Xiu-xia, FAN Xiao-jie, HOU Yan-meng, JIANG Shu-zhen, LU Xuan, YUAN Xue-jun. Effects of Autonomic Nerve on Mast Cell and Histamine Content in Rat Uterus during Peri-implantation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2409-2415.
[4]	SUN Wen-tao, GAO Wen-bo, DAI Mei-ling, CHEN Xiang-xing, YANG Zai-bin, JIANG Shu-zhen, YANG Wei-ren, HUANG Li-bo. Effect of Dietary Fusarium Toxin on Histological Structure, Distribution and Expression of Hsp70 in the Uterus of Post-Weaning Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1035-1043.
[5]	SUN Wen-tao, GAO Wen-bo, DAI Mei-ling, CHEN Xiang-xing, YANG Zai-bin, JIANG Shu-zhen, YANG Wei-ren, HUANG Li-bo. Effect of Dietary Fusarium Toxin on Histological Structure, Distribution and Expression of Hsp70 in the Uterus of Post-Weaning Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1035-1043.
[6]	KUANG Mei-qian, JIN Peng-jin, WANG Ruo-cheng, SUN Ling-wei, WANG Feng, LI Peng, CHENG Zhi-jun, MAO Da-gan. Effect of Food Restriction on the Expression of RGMb and BMP System Members in the Uterus of Hu Ewes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 863-870.
[7]	KUANG Mei-qian, JIN Peng-jin, WANG Ruo-cheng, SUN Ling-wei, WANG Feng, LI Peng, CHENG Zhi-jun, MAO Da-gan. Effect of Food Restriction on the Expression of RGMb and BMP System Members in the Uterus of Hu Ewes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 863-870.
[8]	DING Ya-nan, ZHAO Yue-xiang, WANG Ling-xia, HAN Jin-feng, SONG Ze-he, HU Gui-li, LI Guan-ya, FAN Zhi-yong. Effects of Resveratrol on the Growth, Blood Lipid Metabolism and Antioxidant Capacity of Rats Fed with High Fat Diet [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2216-2224.
[9]	DING Ya-nan, ZHAO Yue-xiang, WANG Ling-xia, HAN Jin-feng, SONG Ze-he, HU Gui-li, LI Guan-ya, FAN Zhi-yong. Effects of Resveratrol on the Growth, Blood Lipid Metabolism and Antioxidant Capacity of Rats Fed with High Fat Diet [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2216-2224.
[10]	LIANG Hong-wei，FENG Bo，ZHU Wen-yu，WANG Jian-fang，ZHANG Tao，MU Xiang. Cultivation and Purification of Rat Pulmonary Microvascular Endothelial Cells in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2143-2150.
[11]	LIANG Hong-wei，FENG Bo，ZHU Wen-yu，WANG Jian-fang，ZHANG Tao，MU Xiang. Cultivation and Purification of Rat Pulmonary Microvascular Endothelial Cells in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2143-2150.
[12]	LI Li-min，WANG Yan，CUI Bei-bei，LIN Min，ZHANG Lei，ZUO Yu-zhu，WANG Jia-xin. The Effectiveness of Mast Cell Pattern Recognition of Recombinant VP1-VP4 of Foot-and-mouth Disease Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1644-1649.
[13]	LI Li-min，WANG Yan，CUI Bei-bei，LIN Min，ZHANG Lei，ZUO Yu-zhu，WANG Jia-xin. The Effectiveness of Mast Cell Pattern Recognition of Recombinant VP1-VP4 of Foot-and-mouth Disease Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1644-1649.
[14]	HUO Sheng-dong，YUAN Qi-jian，LU Jian-xiong，ZANG Rong-xin，YANG Ju-tian，ZHANG Tao-jie，Ayimuguli，LIU Jun-lin，MA Zhong-ren，LONG Run-jun. Melatonin Receptor Expression in Uterus during Pregnancy，Nonpregnancy of Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 239-244.
[15]	HUO Sheng-dong，YUAN Qi-jian，LU Jian-xiong，ZANG Rong-xin，YANG Ju-tian，ZHANG Tao-jie，Ayimuguli，LIU Jun-lin，MA Zhong-ren，LONG Run-jun. Melatonin Receptor Expression in Uterus during Pregnancy，Nonpregnancy of Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 239-244.