不同性别大白猪肌肉全基因组高分辨率单碱基甲基化差异分析

doi:10.11843/j.issn.0366-6964.2018.11.004

Abstract

Abstract:

The aims of this study were to detect the differentially methylated region (DMR) and differentially methylated gene (DMG) and lay a foundation for further analyzing the skeletal muscle development difference between male and female pigs by high resolution and single base genome-wide methylation variance analysis for Large White pigs of different genders. In this study, four 210-day-old Large White pigs (two males and two females) were scanned by the whole genome bisulfite sequencing method (WGBS). The degree of methylation and differentially methylated region in the whole genome DNA of longissimus dorsi(LD) muscle were studied to explore the difference of DNA methylation level between male and female pigs. The results showed that about 5% Cytosine (C) were methylated in the whole genome. The overall methylation level of sows and boars was basically the same. A total of 1 629 DMRs and 841 related DMGs were detected. The DMRs methylation level of sows was higher than that of boars. One hundred and sev-enty one GO terms and 10 related signal pathways were detected by GO and KEGG analysis, respectively, which were showed to be significantly enriched in cell junction, axon guidance, cell adhesion molecule binding, ECM-receptor interaction, and so on. Five candidate genes were screened out in muscle tissues of male and female pigs. This study provided the single base and high resolution genome-wide methylation pattern of male and female Large White pigs. The results provide reference information for the epigenetic study of different genders and screening candidate genes related to muscle development and meat quality.

Key words: gender, muscle, genome-wide, methylation

CLC Number:

S828.2

GUO Tian-fu, ZHANG Zhi-yan, CHEN Dong, YAO Tian-xiong, XIAO Shi-jun, HUANG Lu-sheng. High Resolution and Single Base Genome-wide Methylation Variance Analysis of Muscle of Large White Pigs with Different Sexes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2326-2339.

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High Resolution and Single Base Genome-wide Methylation Variance Analysis of Muscle of Large White Pigs with Different Sexes

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