ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (9): 2005-2014.doi: 10.11843/j.issn.0366-6964.2018.09.022

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Study on Residue Elimination of Altrenogest Oral Solution in Pigs

WANG Shuang1,2, YANG Hai-feng1,2, YU Bo1,3, LI Shi-yang1,3, ZHENG Yu-ji1,3, BU Shi-jin1,3*   

  1. 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;
    3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infections Diseases and Zoonoses, Yangzhou 225009, China
  • Received:2018-01-17 Online:2018-09-23 Published:2018-09-26

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Abstract:

The experiment was conducted to study the residues of altrenogest in swine tissues, preparing for the determination of withdrawal time. The liver and kidney samples were hydrolyzed by β-glucosidase/arysulfatase and extracted by acetonitrile (the muscle and fat samples were extracted by acetonitrile), then the extracting solution were degreased with n-hexane. After being redissolved with ethyl acetate, the supernatant was purified by 10% sodium carbonate solution. Finally, altrenogest was analyzed by LC-MS/MS, and was calibrated by the external method. The results showed that the standard curve was obtained with the correlation coefficients (R2=0.999) in the concentration range of 1-200 ng·mL-1. The limit of quantification for altrenogest was 1.0 μg·kg-1, and the limit of detection for altrenogest was 0.5 μg·kg-1. The recoveries of altrenogest were 68.9%-78.9% at three spiked levels of 1, 100, 200 μg·kg-1 with the relative standard deviations less than 11% (n=6). After the pigs were orally administered altrenogest of 0.4 mg·kg-1 of body weight onetime every day for 18 d, the concentration of in all of the tissues at 6 h was highest; the concentration of altrenogest in fat at 5 d was lower than MRL, while the concentration of altrenogest in muscle and kidney tissues were lower than LOQ; the concentration of altrenogest in all of the tissues at 10 d were lower than LOQ. The order of residue rate was liver > kidney > fat > muscle, and liver was considered as the target organ. The concentration-time dates were analyzed by WT1.4 to get the withdrawal time with 99% upper tolerance limit, the WT advised was 9 d.

Key words: altrenogest, swine tissues, LC-MS/MS, residue depletion

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