基于iTRAQ技术的卵巢静止奶牛血清差异蛋白分析

doi:10.11843/j.issn.0366-6964.2019.05.008

Abstract

Abstract:

This study aimed to explore serum differential proteins in cows with inactive ovaries using iTRAQ technology. The Holstein cows in a intensive dairy farm were determined for energy balance (EB) status by blood β-hydroxybutyric acid (BHBA), and were assigned into inactive ovaries groups (IO, n=50), normal estrus control group (CON, n=50) at 60 to 90 day postpartum by the estrus manifestation, rectal examination and B-ultrasound examination.The isotope-labeled absolute quantification (iTRAQ)/mass spectrometry (MS) combined technique were used to screen differentially expressed proteins,Western blot and ELISA methods were used to identify differentially expressed proteins in serum of dairy cows with inactive ovaries. The results showed that 61 differentially expressed proteins were obtained,GO analysis showed that the biological process consisted of 34 annotations, the molecular function consisted of 15 annotations, the cellular composition consisted of 13 annotations. A total of 57 nodes were obtained from the analysis of protein network interaction. 68 proteins interpreted each other, protein interactions were enriched P<10^-16, 11 signal metabolic pathways were obtained. After screening, it were found that glycolysis/gluconeogenesis, amino acid biosynthesis,glucagon signaling pathway, vitamin digestion and absorption might be related to the occurrence of ovarian quiescence.A total of 14 differentially expressed proteins verified by Western blot and ELISA were associated with inactive ovaries:GPX3, SCGB1D and PKM2 were down-regulated;ADIPOQ, AHSG, APOA4, FETUB, ALDOB, SPAM1, LDHB, RBP4, IGFBP2, ITIH3 and GLYCAM1 were up-regulated.ADIPOQ, IGFBP2 and RBP4 affect follicular development by affecting reproductive hormone biological processes, GPX3 affects follicular development through oxidative stress.

CLC Number:

S823.9⁺1.2

ZHAO Chang, ZHANG Jiang, BAI Yunlong, SUN Shuhan, SONG Yuxi, XIA Cheng. Analysis of Serum Differential Proteins in Cows with Inactive Ovaries Based on iTRAQ Technology[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 972-982.

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Analysis of Serum Differential Proteins in Cows with Inactive Ovaries Based on iTRAQ Technology

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