N-氨甲酰谷氨酸对育成期水貂生长性能、营养物质消化率、氮代谢及血清生化指标的影响

doi:10.11843/j.issn.0366-6964.2018.06.011

Abstract

Abstract:

This study was conducted to study the effects of different dietary N-carbamoylglutamate (NCG) levels on growth performance, nutrient digestibility, nitrogen metabolism and serum biochemical indexes of growing minks. One hundred and fifty healthy weaned Black minks(Mustela vison) with similar body weight were selected and randomly divided into 5 groups with 30 replicates per group and one mink per replicate. These minks were fed with NCG supplementation levels of 0% (group A), 0.02% (group B), 0.06% (group C), 0.10% (group D) and 0.14% (group E), respectively in the experimental diets, NCG was added from June 8^th to September 15^th. The pre-trial lasted for 7 days and the trial for 92 days. From September 12^th, 10 minks (5 male and 5 female) were selected from each group for digestion and metabolism test for 3 days, muck method determination of feed and feces were conducted to measure the contents of dry matter (DM), crude protein (CP) and crude fat (EE) and then to calculate the apparent digestibility and nitrogen metabolism. On the morning of September 15^th, 6 minks(3 male and 3 female) were selected from each group. Blood was collected and the contents of arginine (Arg), nitric oxide (NO), growth hormone (GH), total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase(AST), immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), complement 3 (C3), complement 4 (C4) in serum were determined by ELISA. The results showed that:1) The final weight and average daily gains of female minks fed with NCG were all higher than the control group, that of female minks in group B were the highest, but the differences were not significant between different groups (P>0.05). These indicators of male minks in group B was significantly higher than group A, C and E (P<0.05). The feed/gain of female minks in group B was the lowest, but the differences were not significant between different groups (P>0.05), the feed/gain of male minks in group B was significantly lower than A and E groups (P<0.05). 2) The digestibility of dry matter, crude protein and crude fat of minks fed with NCG were all higher than the control group, but the differences were not significant between different groups (P>0.05). 3) There were no significant differences in nitrogen deposition, net protein utilizable rate and biological value of protein between different groups (P>0.05), but compared with group A, the nitrogen deposition of D group and the net protein utilizable rate, biological value of protein of B group were higher. 4) Compared with group A, the levels of arginine, nitric oxide, growth hormone, total protein, IgA, IgG, IgM, C3 and C4 in serum of minks fed with NCG extremely significantly increased (P<0.01), and group B was all the best, the alanine aminotransferase and aspartate aminotransferase extremely significantly decreased (P<0.01). The results indicated that basal diet supplemented with 0.02% NCG could help the growing minks to gain the average daily weight, reduce the feed/gain, improve the nitrogen deposition, net protein utilizable rate and biological value of protein, and increase the levels of arginine, growth hormone, total protein and immunoglobulin in serum.

CLC Number:

S829.9

DONG Xiao-jing, CUI Kai, MA Ze-fang, LI Jian-dong, LI Ying-mei. Effects of Dietary N-carbamoylglutamate on Growth Performance, Nutrient Digestibility, Nitrogen Metabolism and Serum Biochemical Indexes of Growing Mink[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1194-1203.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.06.011

https://www.xmsyxb.com/EN/Y2018/V49/I6/1194

References

[1] 毛俊舟,王淑楠,彭众,等.精氨酸对仔猪的营养调节作用及最新研究进展[J].猪业科学,2017,34(8):94-96.
MAO J Z,WANG S N,PENG Z,et al.Effect of arginine on nutrition of piglets and latest research progress[J].Swine Industry Science,2017,34(8):94-96.(in Chinese)
[2] 刘凤菊,呙于明,王磊.1~3周龄雌性肉仔鸡精氨酸需要量[J].动物营养学报,2011,23(4):571-577.
LIU F J,GUO Y M,WANG L.Arginine requirement for female broiler chicks ages from 1 to 3 weeks[J].Chinese Journal of Animal Nutrition,2011,23(4):571-577.(in Chinese)
[3] 范苗.添加不同水平的精氨酸对新生仔猪生长性能、免疫性能及胃肠道发育的影响[D].保定:河北农业大学,2011:15.
FAN M.Effects of L-arginine on the growth performance,immune performance and gastrointestinal development in neonatal pigs[D].Baoding:Hebei Argicultural University,2011:15.(in Chinese)
[4] GLEM-HANSEN N.水貂对蛋白质及氨基酸的需求[J].毛皮动物饲养,1995(1):52-54.
GLEM-HANSEN N.Protein and amino acid requirements of minks[J].Journal of Economic Animal,1995(1):52-54.(in Chinese)
[5] WU G Y,BAZER F W,DAVIS T A,et al.Important roles for the arginine family of amino acids in swine nutrition and production[J].Livestock Sci,2007,112(1-2):8-22.
[6] 曹洪战,陈楠,芦春莲.N-氨甲酰谷氨酸对哺乳仔猪生长及内源精氨酸合成代谢的影响[J].畜牧兽医学报,2013,44(11):1844-1850.
CAO H Z,CHEN N,LU C L.Effects of N-carbamylglutamate on growth performance and the endogenous anabolism of arginine of suckling piglets[J].Acta Veterinaria et Zootechnica Sinica,2013,44(11):1844-1850.(in Chinese)
[7] 汤智群,周佳栋,洪伟鸣,等.N-氨甲酰谷氨酸和精氨酸对京海黄鸡肉仔鸡生长性能和血液指标的影响[J].中国畜牧兽医,2015,42(5):1130-1136.
TANG Z Q,ZHOU J D,HONG W M,et al.Effects of dietary N-carbamylglutamate and arginine on growth performance and blood parameters of Jinghai yellow broiler chicks[J].China Animal Husbandry & Veterinary Medicine,2015,42(5):1130-1136.(in Chinese)
[8] 卞宝国,李吕木,钱坤.N-氨甲酰谷氨酸的生物学功能及其在猪生产中的应用[J].中国饲料,2014(1):12-14.
BIAN B G,LI L M,QIAN K.Biological function of N-carbamylglutamate and application in pig production[J].China Feed,2014(1):12-14.(in Chinese)
[9] WU G Y,JAEGER L A,BAZER F W,et al.Arginine deficiency in preterm infants:biochemical mechanisms and nutritional implications[J].J Nutr Biochem,2004,15(8):442-451.
[10] 谢植鑫,苏江伟,刘璐,等.精氨酸生素在猪营养中的研究与应用[J].饲料博览,2014(11):12-15.
XIE Z X,SU J W,LIU L,et al.The research and application in nutrition of the pig in arginine activator additive[J].Feed Review,2014(11):12-15.(in Chinese)
[11] NRC.Nutrient requirements of mink and foxes[S].2nd rev ed.Washington,D.C.:National Academy Press,1982.
[12] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003.
ZHANG L Y.Feed analysis and feed quality inspection technology[M].Beijing:China Agricultural University Press,2003.(in Chinese)
[13] 岳隆耀,王春平,谯仕彦.日粮中添加N-氨甲酰谷氨酸(NCG)对断奶仔猪生长的影响[J].饲料与畜牧,2010(1):15-17.
YUE L Y,WANG C P, QIAO S Y.Effects of N-carbamylglutamate on the growth performance in neonatal pigs[J].Feed Husbandry,2010(1):15-17.(in Chinese)
[14] 王琤韡,瞿明仁,游金明,等.N-氨甲酰谷氨酸对断奶仔猪生长性能、养分消化率及血清游离氨基酸含量的影响[J].动物营养学报,2010,22(4):1012-1018.
WANG C W,QU M R,YOU J M,et al.Effects of N-carbamylglutamate on growth performance,nutrient digestibility and serum free amino acids of weaned piglets[J].Chinese Journal of Animal Nutrition,2010,22(4):1012-1018.(in Chinese)
[15] 王利华,李文立,马泽芳,等.水貂高效养殖关键技术有问必答[M]. 北京:中国农业出版社,2017:70.
WANG L H,LI W L,MA Z F,et al.The questions and answers of efficient breeding of key technologies of minks[M]. Beijing:China Agriculture Press,2017:70.(in Chinese)
[16] FENG J,LIU X,XU Z R,et al.Effects of Aspergillus oryzae 3.042 fermented soybean meal on growth performance and plasma biochemical parameters in broilers[J].Animal Feed Sci Technol,2007,134(3-4):235-242.
[17] 周笑犁,连国琦,孔祥峰,等.N-氨甲酰谷氨酸对断奶环江香猪肠道微生物区系的影响[J].天然产物研究与开发,2014,26(2):263-267.
ZHOU X L,LIAN G Q,KONG X F,et al.Effects of N-carbamylglutamate on intestinal microflora of weaned Huanjiang Mini-pigs[J].Natural Product Research and Development,2014,26(2):263-267.(in Chinese)
[18] 王春平,岳龙耀.N-氨甲酰谷氨酸(NCG)在养猪生产中的应用[J].中国动物保健,2014(12):68-70.
WANG C P,YUE L Y.N-carbamylglutamate research progress in pig production[J].China Animal Health,2014(12):68-70.(in Chinese)
[19] 王琤韡,王仁华.N-氨甲酰谷氨酸在仔猪生产中的研究应用进展[J].粮食与饲料工业,2015(9):49-50,58.
WANG C W,WANG R H.Application of N-carbamylglutamic acid in piglet production[J].Cereal & Feed Industry,2015(9):49-50,58.(in Chinese)
[20] 周玉香,吕玉玲,王洁,等.血液生化指标在动物生产与营养调控研究中的应用概况[J].畜牧与饲料科学,2012,33(5-6):72-73.
ZHOU Y X,LV Y L,WANG J,et al.Application survey of blood biochemical indices in the studies of animal production and nutritional regulation[J].Animal Husbandry and Feed Science,2012,33(5-6):72-73.(in Chinese)
[21] 王越,沈连忠,李波.临床前研究中肝损伤的临床病理指标的选择及意义[J].中国药事,2009,23(8):813-816,825.
WANG Y,SHEN L Z,LI B.Selection and interpretation of clinical pathology indicators of hepatic injury in preclinical studies[J].Chinese Pharmaceutical Affairs,2009,23(8):813-816,825.(in Chinese)
[22] 洪伟鸣,曹飞,宋亮,等.N-氨甲酰谷氨酸对小鼠的亚慢性毒性试验研究[J].基因组学与应用生物学,2015,34(4):731-735.
HONG W M,CAO F,SONG L,et al.Study on subchronic toxicity test of N-carbamylglutamate on mouse[J].Genomics and Applied Biology,2015,34(4):731-735.(in Chinese)
[23] 杨汉春.动物免疫学[M].2版. 北京:中国农业大学出版社,2003:66-140.
YANG H C.Animal immunology[M].2nd ed. Beijing:China Agricultural University Press,2003:66-140.(in Chinese)
[24] 孙皓然,张铁涛,刘志,等.饲粮精氨酸水平对育成期雌性蓝狐生长性能、营养物质消化率、氮代谢及血清生化指标的影响[J].动物营养学报,2015,27(10):3285-3292.
SUN H R,ZHANG T T,LIU Z,et al.Effects of dietary arginine level on growth performance,nutrient digestibility,nitrogen metabolism and serum biochemical indexes of female blue foxes in growing period[J].Chinese Journal of Animal Nutrition,2015,27(10):3285-3292.(in Chinese)
[25] 万春孟,张铁涛,吴学壮,等.饲粮L-精氨酸水平对育成期水貂氨基酸消化率、血清氨基酸含量及血清生化指标的影响[J].动物营养学报,2015,27(12):3789-3796.
WAN C M,ZHANG T T,WU X Z,et al.Effects of dietary L-arginine level on amino acid digestibility,serum amino acid content and serum biochemical indices of growing minks[J].Chinese Journal of Animal Nutrition,2015,27(12):3789-3796.(in Chinese)

Effects of Dietary N-carbamoylglutamate on Growth Performance, Nutrient Digestibility, Nitrogen Metabolism and Serum Biochemical Indexes of Growing Mink

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YU Xiuju, ZHANG Min'ai, HU Yanjiao, ZHU Zhiwei, WANG Haidong, YANG Lihua, FAN Kuohai. Isolation, Expression and Stability Analysis of Bacteriocins from Bacillus subtilis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 323-333.
[2]	LI Haodong, MIN Xiangyu, ZHOU Ya, ZHANG Heyang, ZHENG Junjun, LIU Linling, WANG Ping, WANG Yanmei, YANG Fuhe, WANG Guiwu. Comparison of Prediction Accuracy of Genomic Selection for Growth-related Traits in Sika Deer (Cervus Nippon) based on GBLUP and other Models [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 608-616.
[3]	ZHAO Yongwei, NIU Yu, HE Jintian, ZHANG Lili, WANG Chao, WANG Tian. Effects of Dietary Dihydroartemisinin Supplementation on Insulin Level and Liver Development in Intrauterine Growth Retardation Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1628-1636.
[4]	ZHANG Lihuan, MA Yueyue, LIU Wenyan, LAN Wutao, ZHU Zhiwei. microRNA-96-5p Targets MITF Gene in Alpaca Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1229-1237.
[5]	LI Lanhui, DU Xiaolong, WANG Qi, GE Linhan, ZHANG Lechao, LI Xuemei, LI Xianglong. Exploration of Promoter Activity and Analysis of 5'UTR Sequence of Mink DCT Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 524-533.
[6]	NAN Wei-xiao, ZHANG Hai-hua, SI Hua-zhe, MU Lin-lin, LOU Yu-jie, LI Guang-yu. The Effects of Excessive Vitamin A Supplementation on Growth Performance and Serum Biochemical Indices in Growing Male Minks [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2425-2434.
[7]	WANG Jing, ZHANG Hai-hua, XU Yi-nan, LI Ren-de, ZHANG Xue-lei, CAI Xi-heng, LI Guang-yu. Effects of Dietary Vitamin D and Calcium Levels on Production Performance, Serum Biochemical Parameters and Organ Indexes of Male Standard Mink during Winter Fur-growing Period [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 986-995.
[8]	WU Xue-zhuang, YANG Ying, LIU Hui-tao, GAO Xiu-hua, YANG Fu-he, DAI Si-fa, XING Xiu-mei. Effects of Dietary Copper Levels on Copper Metabolism and Serum Biochemical Indices in Late Growing Male Mink [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 746-753.
[9]	ZHANG Hai-hua, WANG Shi-yong, NAN Wei-xiao, SI Hua-zhe, LI Guang-yu. Effects of Dietary Iron Levels on Production Performance,Blood Serum Biochemical Parameters and Organ Index of Mink [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1557-1564.
[10]	ZHONG Wei, ZHANG Ting, LUO Jing, WANG Zhuo, YUE Zhi-gang, LIU Xue-qing, LI Guang-yu. Effects of Dietary n-6/n-3 PUFA Ratio on the Body Fat Deposition,Body Fatty Acid Composition and Serum Biochemical Parameters in Male Arctic Foxes during the Winter Fur-growing Period [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1054-1065.
[11]	HU Jia-jia, XIONG Xian-rong, SHI Xian, CAI Wen-yi, LAN Dao-liang, LI Jian. Cloning and Expression of RGS1 Gene in Yak Ovary during the Estrous Cycle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 252-259.
[12]	XU Gui-li，ZHANG Wen-xiang，DUAN Ling-xin，GONG Yuan-fang，GE Mu-xiang，LIU Xie-rong，WANG Shu-peng，GUO Xin-ling，LIU Zheng-zhu. Study on Adjacent Variation of c.40A>C and c.41C>T of Fox MC1R Gene in Different Populations [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2020-2026.
[13]	GAO Shu-yuan，YU Xiu-ju，CHENG Jia-qi，SONG Ya-jun，ZHAO Bing-ling，NIU Shu，CAO Xiao-rui，HE Xiao-yan. Different Expression of RSPO2 and β-catenin in the Different Sites of Alpaca Skins [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 931-937.
[14]	ZHANG Ya-ling，XU Dong-mei，DU Bin，XIE Jian-shan，FAN Rui-wen，YU Xiu-ju，ZHU Zhi-wei，DONG Chang-sheng. The Influences of Over-Expressing miR-193b on MITF and TYR in Melanocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 938-943.
[15]	ZHOU Ning，ZHANG Hai-hua，LYU Zhi-chao，ZHANG Ting，HUANG Jian，SUN Hao-ran，YIN Yun-hou，LI Guang-yu，YAN Chang-guo. Effects of Diets with Different Zinc Levels on the Selected Serum Biochemical Indexes and Organ Indexes of Minks during the Winter Hair Period [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1988-1994.