副猪嗜血杆菌TbpA对仔猪的免疫效力评价

doi:10.11843/j.issn.0366-6964.2018.03.016

Abstract

Abstract:

The aim of this study was to investigate the immune efficacy of the transferrin-binding protein A of HPS and to lay a foundation for research and development of new vaccine. The piglets were immunized with the inactivated whole cell vaccine from HPS serotype 13 and recombinant protein TbpA(0.5 and 0.25 mg·4 mL^-1). The immunization was conducted at week 0, 2, 4 and then challenged with HPSLJ3 (7 LD₅₀). Serum antibody (IgG) and cytokines (IL-5, IL-10, IFN-γ, TNF-α) levels were measured by ELISA and pathological examination was performed. Results were as follows:Immunization and challenge assay demonstrated the recombinant protein TbpA could induce high levels of antibody (IgG) and cytokines. The protective efficacy of the inactivated whole cell and TbpA (0.5 mg·4mL^-1) vaccines was 100%. There was a significant difference between the pathological changes and the control group. TbpA can stimulate the humoral and cellular immunity, and produce powerful immune protection. These results suggested that TbpA is a potential candidate for developing a novel HPS vaccine.

CLC Number:

S855.12

YAO Yan-bin, CHEN Zhang, CHU Xia-fei, WEI Jian-zhong, SUN Pei, LI Yu. Immune Efficacy of Transferrin-binding Protein A of Haemophilus parasuis in Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 588-596.

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Immune Efficacy of Transferrin-binding Protein A of Haemophilus parasuis in Piglets

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