体外法探究果寡糖对猪后肠微生物代谢苯丙氨酸和色氨酸的影响

doi:10.11843/j.issn.0366-6964.2018.03.012

Abstract

Abstract:

This experiment aimed to study the effect of FOS on metabolism of phenylalanine(Phe) and tryptophan (Trp) in pig hindgut bacteria fermentation broths in vitro. Ileum, cecum and colon chyme in pigs were used as inoculum. Phe and Trp concentrations were kept at 10 mmol·L^-1 in fermentation broth. The dose of FOS was 0, 0.5 and 0.75 g, respectively. Fermentation samples collected at 24 h and 37℃ were used to measure concentrations of amino aicds, ammonia nitrogen (NH₃-N), microbial crude protein (MCP), indole and skatole. Real-time PCR was used to quantify the bacterial numbers. The results showed that the fermentation characteristic of Phe and Trp differed in different gut locations. The degradation rate of Phe was significantly affected by FOS(P<0.05). The concentrations of NH₃-N, MCP, skatole and number of total bacterial were significantly affected by FOS (P<0.05), while indole was not affected by FOS (P>0.05). In 0.75 g FOS group, Trp degradation rate decreased significantly in the hindgut intestine (P<0.05). Trp was the major factor affecting indole and skatole production in fermentation broths. Collectively, FOS could alter the metabolic patterns of phenylalanine and tryptophan by gut bacteria, and decreased the conversion of tryptophan to skatole.

CLC Number:

S828.5

MU Chun-long, MA Mei-lei, HE Xiang-yu, ZHU Wei-yun. Effect of FOS on Metabolism of Phenylalanine and Tryptophan in Pig Hindgut Bacteria Fermentation Broths in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 559-564.

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Effect of FOS on Metabolism of Phenylalanine and Tryptophan in Pig Hindgut Bacteria Fermentation Broths in vitro

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