禽呼肠孤病毒强毒株和弱毒疫苗株感染Vero细胞的蛋白质组学研究

doi:10.11843/j.issn.0366-6964.2016.02.016

Abstract

Abstract:

To discover protein expression changes in infected Vero cells with avian reovirus (ARV) virulent strain S1133 and attenuated vaccine strain aS1133，respectively，two dimensional gel electrophoresis (2-DE) and mass spectroscopy (MS) technologies were utilized to separate and identify the total proteins of Vero cells inoculated with ARV S1133 or aS1133 as well as control cells at desired time points post infection.The identification results showed that total 44 differently expressed protein dots were identified，including α-enolase，Prx-4，hnRNPs，tubulin，protein phosphatase，transcription elongation factor.These differentially expressed proteins were involved in various biological functions，including signaling transduction，cytoskeleton，metabolism，protein disfolding，cell proliferation and apoptosis.The results of quantitative RT-PCR validated the mRNA expression of α-enolase，Prx-4，hnRNPs and tubulin were in agreement with that of proteomic analysis.The present data highlighted protein expression differences in infected cells with ARV virulent strain and attenuated vaccine strain，which would contribute to further elucidate the interaction between ARV and hosts.

CLC Number:

S852.659.4

HUANG Li，XIE Zhi-xun，XIE Li-ji，DENG Xian-wen，XIE Zhi-qin，FAN Qing，LUO Si-si，HUANG Jiao-ling，ZENG Ting-ting，ZHANG Yan-fang，WANG Sheng. Proteomic Analysis of Vero Cells Infected by Avian Reovirus Virulent Strain and Attenuated Vaccine Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 331-339.

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Proteomic Analysis of Vero Cells Infected by Avian Reovirus Virulent Strain and Attenuated Vaccine Strain

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