ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (1): 156-162.doi: 10.11843/j.issn.0366-6964.2015.01.020

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Effect of Ginsenoside Rb1 Induce Microvascular Vasomotion and the Effect on Sensitive Threshold of Rat Myocardial Microvascular Endothelial Cells

WANG Xin1,FENG Bo2,SUN Xiang-wan1,LI Ping-li1,DONG Hong1*,MU Xiang1*   

  1. (1.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing University of Agriculture,Beijing 102206,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)
  • Received:2014-10-28 Online:2015-01-23 Published:2015-01-23

Abstract:

The aim of this study was to investigate the effects of Ginsenoside Rb1(GSRb1) induce Hegu acupoint microvascular vasomotion and the effect on sensitive threshold of Rat myocardial microvascular endothelial cells(RMMECs).Microvascular vasomotion was detected by laser doppler blood flow monitor combined with iontophoresis device.The changes of calcium in the RMMECs were measured by laser scanning confocal microscopy combined with calcium fluorescent probe (Fluo-3AM).The results were as follows:The minimum amount of ATP could induce calcium transient increase in RMMECs was 0.5 μg•mL-1,the dose of significant difference was 0.7 μg•mL-1 and the minimum amounts of GSRb1 inducing calcium changes in RMMECs was 8 μg•mL-1.After incubation 8 μg•mL-1 of GSRb1,the minimum amount of ATP could induce calcium transient increase in RMMECs was 1.5 μg•mL-1.Eight μg•mL-1 of GSRb1 can inhibit the ATP-induced intracellular calcium transient rise,that meant,GSRb1 increased the sensitive threshold of RMMECs to ATP.

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