关键词: 兔出血症病毒, 衣壳蛋白(VP60)基因, RT-PCR, 生物信息, 序列分析

Abstract: The capsid protein VP60 gene of rabbit hemorrhagic disease virus（RHDV）YL Strain was cloned by RT-PCR and then sequenced. YL Strain was the first RHDV strain being sequenced in the Northwest of China. The results showed that the nucleotide sequence of VP60 gene was 1 740nt in length and encoding a protein of 580 aa.The sequence had been submitted to GenBank (the accession number is DQ530363)We downloaded all other 39 sequences of published RHDV isolates from GenBank and then constructed phylogenetic tree and drawn homology and divergence form by bioinformatics software. The results indicated that the VP60 gene of RHDV was very conservative, and the homology of the nucleotide among different isolates exceeded 90%, but they can be divided into 5 gene groups. The gene group and region had negative connection.All Chinese RHDV isolates belonged to the Ⅴ gene group eccept WX84 Strain. In this group, the hereditary distance between the strains isolated in recent years and NJ85 Strain was very close. The RHDV strains of China isolated in recent years probably come from the same strain NJ85. Meanwhile, the secondary structure and antigenic index of four strains from different areas of China were predicted and analyzed. The results revealed that there were certain differences in secondary structure, but the antigenicity was identical

Key words: rabbit hemorrhagic disease virus, capsid protein (VP60) gene, RT-PCR, bioinformatics, sequence analysis