肉鸡肠道NHE2 mRNA表达的组织特异性与发育性变化

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (1): 42-47.

肉鸡肠道NHE2 mRNA表达的组织特异性与发育性变化

王修启;邹仕庚;左建军;苏海林;黄志毅;冯定远

华南农业大学动物科学学院，广州 510642

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-24 发布日期:2008-01-24

Regional and Ontogenetic Expression of NHE2 mRNA in Intestine of Broiler

WANG Xiu-qi;ZOU Shi-geng;ZUO Jian-jun;SU Hai-lin;HUANG Zhi-yi;FENG Ding-yuan

College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-01-24 Published:2008-01-24

摘要/Abstract

摘要： 选用遗传背景相同的1日龄父母代雄性Arbor Acre (AA)肉雏鸡120羽，随机分为4个重复，采用相对定量RT-PCR方法，以30日龄AA肉鸡肠道RNA为模板，研究肉鸡肠道钠/氢交换载体2（Sodium hydrogen exchanger 2, NHE2）mRNA表达的组织特异性；以AA肉鸡十二指肠和空肠RNA为模板，研究肉鸡肠道NHE2 mRNA表达的发育性变化。结果显示：①AA肉鸡十二指肠和空肠NHE2 mRNA的表达丰度显著高于回肠和结直肠(P<0.05)，而十二指肠和空肠之间、回肠和结直肠之间无显著差异（P>0.05）；②AA肉鸡NHE2 mRNA在十二指肠及空肠中的表达具有相同的发育模式，2~16日龄升高，30~44日龄下降，55日龄略微回升；在16和30日龄时的表达丰度显著高于2、44和58日龄（P<0.05）。以上结果说明：AA肉鸡肠道近端NHE2 mRNA的表达丰度显著高于远端(P<0.05)。AA肉鸡十二指肠及空肠NHE2 mRNA的表达具有相同的发育模式，表明NHE2 mRNA表达受到发育阶段的调控，且在十二指肠和空肠间具有稳定性。

关键词: 肉鸡, 肠道, NHE2 mRNA, 组织特异性, 发育性表达

Abstract: 120 1-day-old parental male Arbor Acre (AA) broilers were randomly divided into 4 replicates, respectively. Regional expression of sodium hydrogen exchanger 2 (NHE2) mRNA in different intestinal tract segments of AA broiler at 30-day-old and ontogenetic expression of NHE2 mRNA in duodenum and jejunum of AA broilers were determined by relative quantitative RT-PCR. It was found that: (1) Expression of NHE2 mRNA in duodenum, jejunum, ileum and colorectum was declined. The abundance of NHE2 mRNA in duodenum and jejunum was higher than that in ileum and colorectum (P<0.05)，respectively. (2)Ontogenetic patterns of NHE2 mRNA were same between duodenum and jejunum. The abundance of NHE2 mRNA was increased from 2- to 16-day-old, decreased from 30- to 44-old-day and increased again on 58-day-old. The abundance of NHE2 mRNA on 16- and 30-day-old were higher than that on 2-, 44- and 58-day-old (P<0.05)，respectively. These results indicate that regional expression of NHE2 mRNA in intestine of AA broilers was significantly declined along the downward intestine from proximal intestine to distal intestine (P<0.05). Ontogeny of NHE2 mRNA in AA broilers had the same pattern in duodenum and jejunum, indicating that NHE2 mRNA expression could be regulated by developmental stage.

Key words: broiler, intestine, NHE2 mRNA, regional expression, ontogenic expression

王修启;邹仕庚;左建军;苏海林;黄志毅;冯定远. 肉鸡肠道NHE2 mRNA表达的组织特异性与发育性变化[J]. 畜牧兽医学报, 2008, 39(1): 42-47.

WANG Xiu-qi;ZOU Shi-geng;ZUO Jian-jun;SU Hai-lin;HUANG Zhi-yi;FENG Ding-yuan. Regional and Ontogenetic Expression of NHE2 mRNA in Intestine of Broiler[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(1): 42-47.

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