复合黏膜免疫佐剂对鸡小肠细胞免疫的影响

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (1): 85-90.

复合黏膜免疫佐剂对鸡小肠细胞免疫的影响

张小飞;杨倩

南京农业大学农业部动物生理生化重点开放实验室，南京 210095

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-24 发布日期:2008-01-24

pound Mucosal Immune Adjuvants on Cellular Immune Responses of Small Intestine in Chicken

ZHANG Xiao-fei;YANG Qian

Key Laboratory of Animal Physiology and Biochemistry of Ministry of Agriculture，Nanjing Agricultural University， Nanjing 210095， China

Received:1900-01-01 Revised:1900-01-01 Online:2008-01-24 Published:2008-01-24

摘要/Abstract

摘要： 分别应用复合黏膜免疫佐剂Ⅰ和Ⅱ与新城疫Ⅳ系弱毒苗混合（佐剂Ⅰ组和佐剂Ⅱ组），经口免疫鸡后，研究鸡小肠黏膜局部CD3+T细胞、上皮内淋巴细胞和干扰素-γ（Interferon-γ，IFN-γ）mRNA表达水平的变化。结果表明，在整个免疫期内CD3+T细胞和上皮内淋巴细胞数量都呈上升趋势，在首免后第3和第5周时十二指肠CD3+T细胞佐剂Ⅱ组比佐剂Ⅰ组显著增加（P<0.05），空肠CD3+T细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加（P<0.01），第7周时Peyer’s斑上皮内淋巴细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加（P<0.01）；整个免疫期内佐剂Ⅱ组和佐剂Ⅰ组IFN-γ mRNA表达水平呈下降趋势，表达量较低。本研究表明2种复合黏膜免疫佐剂都能明显增加黏膜局部免疫细胞的数量，提高小肠局部黏膜免疫力。

关键词: 复合黏膜免疫佐剂, CD3+T细胞, 上皮内淋巴细胞, 干扰素-γ

Abstract: The chicken were immunized orally with NDV strain LaSota vaccine containing two kinds of compound mucosal immune adjuvant(Group adjuvant Ⅰ and Ⅱ) respectively. The CD3+T lymphocytes, intraepithelial lymphocytes(IEL) and IFN-γ mRNA expression of small intestine in chicken were investigated. The results showed that the number of CD3+T lymphocytes and IELs increased during the period of immunity. The number of CD3+T lymphocytes of Group adjuvant Ⅱ were higher than that of Group adjuvant Ⅰ (P<0.05) in duodenum and significantly higher (P<0.01) in jejunum at the 3rd week and 5th week after vaccination. The number of IELs of Group adjuvant Ⅱ were significantly higher than that of Group adjuvant Ⅰ (P<0.01) in Peyer’s Patch at the 7th week after vaccination. The IFN-γ mRNA expression of two compound adjuvant groups decreased after vaccination. The results demonstrated that compound adjuvants could induce high level cellular immune response and promote intestine mucosal immunity in chicken.

Key words: compound mucosal immune adjuvant, CD3+T lymphocytes, intraepithelial lymphocytes, IFN-γ

张小飞;杨倩. 复合黏膜免疫佐剂对鸡小肠细胞免疫的影响[J]. 畜牧兽医学报, 2008, 39(1): 85-90.

ZHANG Xiao-fei;YANG Qian. pound Mucosal Immune Adjuvants on Cellular Immune Responses of Small Intestine in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(1): 85-90.

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