关键词: 鸡, H-FABP, A-FABP, 基因表达, 肌内脂肪

Abstract: Four chicken breeds, including Beijing Fatty, Dwarf, White Leghorn and Arber Acres chickens were used as experimental populations. RT-PCR and real-time fluorescence quantity PCR technique were used to study genetic expression in heart and adipocyte fatty acidbinding protein (H-FABP, A-FABP) genes at 56, 90 and 120 day of age. The associations between genetic expression level and intramuscular fat content and slaughter traits were analyzed by using partial correlated analysis. Statistical analyses showed, for genes expression, the H-FABP mRNA level is significant increasing and A-FABP mRNA level is markedly reducing with age rise. Breeds had remarkably effect on the H-FABP and A-FABP genes expression. Moreover, the sexes had a significant effect on the A-FABP mRNA expression but not H-FABP gene’s. The H-FABP gene expression levels were significantly negative correlation with IMF contents and carcass weight, however, A-FABP mRNA level had a significantly effect on the carcass weight but not IMF content in Beijing Fatty, White Leghorn and Arber Acres chicken . The A-FABP mRNA expression was negatively related significantly with IMF% and H-FABP mRNA level had　marked relation with carcass weight in Dwarf chicken.

Key words: chicken, H-FABP, A-FABP, gene expression, intramuscular fat