生长猪小肠钠依赖型磷摄入特性的研究

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (1): 108-112.

生长猪小肠钠依赖型磷摄入特性的研究

孙杰;张铁鹰;陈志伟;韩进诚;刘志伟

1.山东理工大学生命科学学院，淄博 255049；2. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室，北京 100094；3.西北农林科技大学动物科技学院，杨凌 712100；4. 河北农业大学动物科技学院，保定 071001

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-24 发布日期:2008-01-24

Stduy on Characteristics of Sodium-dependent Phosphate Intake in Small Intestine of Growing Pig

SUN Jie;ZHANG Tie-ying;CHEN Zhi-wei;HAN Jin-cheng;LIU Zhi-wei

1College of Life Science, Shandong University of Technology, Zibo 255049,China; 2State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094,China; 3College of Animal Science and Technology, Northwestern A & F University , Yangling 712100,China; 4College of Animal Science and Technology, Hebei Agricultural University, Baoding, 071001,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-01-24 Published:2008-01-24

摘要/Abstract

摘要： 选取6头体重接近的3月龄生长期大白猪，屠宰后刮下肠黏膜，提取3段小肠上皮细胞刷状缘膜囊泡（Brush-border membrane vesicles, BBMV）。在室温条件下，研究各段小肠BBMV磷的摄入量，pH和钠离子依赖性，并测定BBMV磷吸收动力学参数。结果表明，生长猪BBMV对磷的主动吸收和被动扩散在酸性pH下均受到抑制。钠离子水平影响BBMV磷的主动吸收。pH7.4条件下，测得钠依赖型磷转运系统Hill系数（napp）为2.07，即转运1个磷酸根，至少有2个钠离子与其协同转运。不同肠段BBMV磷的主动吸收并没有显著差异。pH7.4条件下，生长猪钠依赖型磷转运系统对磷的亲和力常数（Km）为（0.43±0.09）mmol/L。最大转运速度（Vmax）为（2.25±0.19）nmol/(mg蛋白·15 s)。

关键词: 生长猪, 刷状缘膜囊泡, 磷摄入

Abstract: Six 3-month-old growing Yorkshire pigs were killed to obtain mucosa, which used to prepare epithelium brush-border membrane vesicles (BBMV). The dependence of Pi uptake on pH and sodium concentration were discussed and kinetics of Na⁺-dependent Pi uptake into BBMV of growing pig were estimated by the measurement of phosphate uptake into BBMV within 15 s at room temperature. Results showed that acid pH made a negative effect on both sodium-dependent phosphate uptake and passive diffusion. Phosphate uptake into BBMV was strongly dependent on sodium concentration. Hill coefficient (napp) of sodium-dependent phosphate cotransport system was 2.07, which indicated that at least two sodium ion was transported with each phosphate ion. The differences of Pi uptake into BBMV between different intestinal segments were not significant. On the condition of varying Pi concentration (0-2.0 mmol/L) and pH7.4, the Pi concentration that yielded halfmaximal transport (apparent Km) estimated was (0.43±0.09)mmol/L in Yorkshire BBMV. The maximal phosphate uptake into BBMV was (2.25±0.19)nmol/(mg protein·15 s).

Key words: growing pig, BBMV, Pi uptake

孙杰;张铁鹰;陈志伟;韩进诚;刘志伟. 生长猪小肠钠依赖型磷摄入特性的研究[J]. 畜牧兽医学报, 2008, 39(1): 108-112.

SUN Jie;ZHANG Tie-ying;CHEN Zhi-wei;HAN Jin-cheng;LIU Zhi-wei. Stduy on Characteristics of Sodium-dependent Phosphate Intake in Small Intestine of Growing Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(1): 108-112.

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