关键词: 鹌鹑, 原始生殖细胞, 迁移, 聚集

Abstract: This paper was conducted to study the migration and accumulation of primordial germ cells (PGCs) in the quail earlier embryo, aiming to provide the base of transgenic technique. The distribution and amount of PGCs at different stages were identified specially by the monoclonal antibody, QH1. The results showed that quail PGCs originated from the area opaca of unincubated blastoderm, and then transferred to area pellucida and the germinal crescent area. About 27 h post-incubation, a few PGCs first appeared in blood vessels of the area pellucida, where a great lot of PGCs accumulated at 36 h post-incubation. Subsequently, the single and mass PGCs were found from the head to omphalo mesenteric and mainly settled down the mesenchymal blood vessels of head. The PGCs amount at different stages were different, and had two periods of maximum in whole stages, namely, primitive streak(6 h post-incubation) and tenth somite (36 h post-incubation). The results indicated that QH1 could identify the PGCs in the quail earlier unincubated embryo, where the PGCs distributed randomly, and there founded two periods of maximum proliferation in whole stages.

Key words: quail, primordial germ cells, migration, accumulation