关键词: 猪瘟病毒, microRNA, 猪脐静脉血管内皮细胞

Abstract: The aim of the study is to find out the microRNAs that acted on classical swine fever virus (CSFV) in swine umbilical vein endothelial cells (SUVECs). CSFV 3′ and 5′untranslated region (UTR) were cloned, and then were inserted into psiCHECH^TM-2 plasmid that carrying Firefly and Renilla luciferases reporter genes; the microRNAs that acted with CSFV were forecasted through bioinformatics analysis; then the recombinant plasmids and inhibitors of predicted microRNA were cotransfected into SUVECs. The activity of Firefly and Renilla luciferases was detected by Luminometer. The PCR products of CSFV 5′UTR（373 bp） and 3′UTR (252 bp) were detected by 10 g·L^-1 agarose electrophoresis consistent with predicted size, from which four predicted microRNAs (ssc-miR-let7c, ssc-miR-106a, ssc-miR-18, ssc-miR-139) were selected. The CSFV 3′UTR is the important action site of microRNA in SUVECs. The four microRNAs mentioned above had different inhibitory effect on CSFV 3′UTR, of which the ssc-miR-18 play a more important role.

Key words: classical swine fever virus, microRNA, SUVECs