关键词: 鸽, 冠状病毒, RT-PCR, S1基因, 分子进化分析

Abstract: The S1 gene of the pigeon original coronavirus (PSH) which was first isolated and identified in China was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method, then it was cloned and sequenced. The results showed that the S1 gene of PSH was 1 626bp and encoded a polypeptide of 541 amino acids. The cleavage site sequence of S1 protein of PSH contains five consecutive basic amino acids, namely, Arg-Arg-Phe-Arg-Arg which is similar to the most IBV strains. Sequence data demonstrated that the complete S1 amino acid of PSH shared 75.0%-99.6% similarity with the published S1 sequences of M41,H52,H120,Beau,Gray,Holte,Conn,SH1,SH2,SH5 and SH6 IBV strains.But it only shared 24.7%,25% similarity with TCV Gh and G1 strain respectively. Based on these findings, the virus has been tentatively identified as a novel pathogenic field IBV strain, generated through recombination.

Key words: pigeon, coronavirus, RT-PCR, S1 gene, phylogenetic analysis