女黄颗粒对免疫抑制模型小鼠脾组织病理及细胞周期的影响

doi:10.11843/j.issn.0366-6964.2018.12.027

摘要/Abstract

摘要：

为分析女黄颗粒对免疫抑制小鼠脾组织病理学及细胞周期的影响，选取50只健康昆明小鼠，随机分为空白组、模型组和女黄颗粒高、中、低剂量组，每组10只。除空白组外，其余几组采用腹腔注射环磷酰胺（80 mg·kg^-1，1次·d^-1，连续5 d）复制免疫抑制模型，复制模型成功后，给各组分别灌胃生理盐水（模型组）或女黄颗粒，连续7 d，以考察女黄颗粒对免疫抑制小鼠脾组织病理学及细胞周期的影响。结果显示：注射环磷酰胺后，小鼠脾指数降低，血液白细胞数、淋巴细胞百分比及单核细胞百分比降低，脾组织受到损伤，脾细胞G0/G1期细胞百分比明显减少，S期细胞百分比增加。给药后，脾指数增加，血液白细胞数、淋巴细胞百分比及单核细胞百分比升高，脾组织结构得到改善，脾细胞G0/G1期细胞百分比明显增加，S期细胞百分比减少，其中中剂量效果明显。女黄颗粒可以改善免疫抑制小鼠的脾的组织结构，使G0/G1期细胞百分比增加，S期细胞百分比减少，增加小鼠脾指数。

关键词: 女黄颗粒, 免疫抑制, 脾, 细胞周期

Abstract:

This study was conducted to analyze the effects of Nühuang granule on histopathology and cell cycle in spleen of immunosuppressed mice. Fifty healthy Kunming mice were randomly divided into blank group, model group, and high-, middle-, and low-dose Nühuang granule groups, with 10 rats in each group. All groups were treated with intraperitoneal injection of cyclophosphamide (80 mg·kg^-1, once a day and for 5 days) to replicate the immunosuppressive model except for the blank group. After model replicated successfully, each group was gavaged with normal saline or drugs for 7 days consecutively. After injection of cyclophosphamide, the spleen index, the number of blood leukocytes, the percentage of lymphocytes and monocytes of mice were decreased. Spleen tissues were damaged and the percentage of G0/G1 phase cells was reduced, and the cells in S phase was increased significantly. While the spleen index, number of blood leukocyte, percentage of lymphocyte and mononuclear of mice were increased, spleen tissue structure was improved, the percentage of spleen cells in G0/G1 phase was increased significantly, and the cells in S phase decreased after Nühuang granule was gavaged to the test mice. Among them, the effect of intermediate dose group was best. Nühuang granule can improve the spleen tissue structure in the immunosuppressed mice. It also can increase the percentage of G0/G1 phase cells, decrease the percentage of S phase cells, and increase the spleen index of test mice.

Key words: Nühuang granules, immunosuppressive, spleen, cell cycle

中图分类号:

S853.9

闫志强, 郑华, 朱买勋, 付文贵, 张邑帆, 翟少钦. 女黄颗粒对免疫抑制模型小鼠脾组织病理及细胞周期的影响[J]. 畜牧兽医学报, 2018, 49(12): 2771-2776.

YAN Zhi-qiang, ZHENG Hua, ZHU Mai-xun, FU Wen-gui, ZHANG Yi-fan, ZHAI Shao-qin. Effect of Nühuang Granules on Spleen Histopathology and Cell Cycle of Immunosuppressive Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2771-2776.

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