畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (7): 1550-1557.doi: 10.11843/j.issn.0366-6964.2018.07.026

• 研究简报 • 上一篇    下一篇

LRP6和VEGFR2在牦牛肺的分布

马俊兴, 何俊峰*, 崔燕, 张倩, 刘鹏刚   

  1. 甘肃农业大学动物医学院, 兰州 730070
  • 收稿日期:2017-11-23 出版日期:2018-07-23 发布日期:2018-07-19
  • 通讯作者: 何俊峰,副教授,E-mail:hejf@gsau.edu.cn
  • 作者简介:马俊兴(1993-),男,河北威县人,硕士,主要从事动物组织学与胚胎学研究,E-mail:17361596112@163.com
  • 基金资助:

    甘肃农业大学青年导师基金资助项目(GAU-QNDS-201708);甘肃农业大学动物医学院教研产学支持计划资助项目(JYCXKX003)

The Distribution of LRP6 and VEGFR2 in Yak Lungs

MA Jun-xing, HE Jun-feng*, CUI Yan, ZHANG Qian, LIU Peng-gang   

  1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-11-23 Online:2018-07-23 Published:2018-07-19

摘要:

旨在探究LRP6和VEGFR2在不同年龄段牦牛肺组织中的表达和定位,以及两者对牦牛肺低氧适应性结构形成和可能的调控作用。选取新生(1~7日龄)、半岁(5~6月龄)、成年(3~6岁)和老年(7~10岁)健康牦牛肺组织样品各5头份,采用酶联免疫吸附试验、Western-blot和免疫组织化学方法对不同年龄的牦牛肺组织中LRP6和VEGFR2的表达量和准确分布位置进行研究。结果显示:LRP6和VEGFR2在初生和半岁牦牛组之间的表达量均差异不显著(P>0.05),初生、半岁与老年组牦牛的表达量均差异显著(P<0.05),成年和老年牦牛组之间的表达量差异显著(P<0.05)。LRP6主要分布于肺内支气管和其分支的上皮细胞以及肺泡Ⅱ型细胞,其表达较强;少量分布在肺血管内皮细胞和管壁平滑肌细胞,表达较弱;VEGFR2主要分布于肺内支气管及其分支的上皮细胞、管壁平滑肌细胞和肺泡隔细胞以及肺泡Ⅰ型细胞中,其表达较强;在肺血管内皮细胞和平滑肌细胞内也有分布,但表达较弱。综上表明,LRP6和VEGFR2不仅在不同年龄段的牦牛肺组织中表达量具有差异性,而且它们对高原环境下牦牛肺适应低氧适应性的结构形成和维持可能的调控具有重要的意义。

关键词: 牦牛, 肺, LRP6, VEGFR2, 适应性

Abstract:

This experiment was conducted to investigate the expression and localization of LRP6 and VEGFR2 in yak lungs of different ages and their roles in yak lung hypoxia adaptive structure formation and the possible regulation. Newborn(1-7 days old, n=5), juvenile(5-6 months old, n=5), adult(3-6 years old, n=5) and old(7-10 years old, n=5) yaks were selected as the subjects, the expression and percise location of LRP6 and VEGFR2 in yak lung tissue of different age groups were detected by ELISA, Western-blot and immunohistochemistry. The results showed that the expression of LRP6 and VEGFR2 between the newborn and juvenile yak groups was not significant different(P>0.05); the expression between newborn, juvenile and old yak groups was significant different(P<0.05); the expression between the adult and old yak groups was significant different(P<0.05). The LRP6 was mainly distributed in the lungs of branches and their branches epithelial cells and alveolar type Ⅱ cells and the expression was stronger; the expression in pulmonary vascular endothelial cells and vascular wall smooth muscle cells was weak. The VEGFR2 was mainly distributed in the epithelial cells of the bronchial lung and its branches, wall smooth muscle cells and alveolar septum and alveolar typeⅠcells and the expression was stronger; the expression in pulmonary vascular endothelial cells and smooth muscle cells was weak. The results suggested that LRP6 and VEGFR2 not only had different expression levels in yak lung tissues of different ages, but also had important implications for the regulation of the formation and maintenance of hypoxic adaptative structures in the lungs of yak in the plateau environment.

Key words: yak, lung, LRP6, VEGFR2, adaptability

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