piRNA的形成及其在雌性动物中生物学功能研究进展

doi:10.11843/j.issn.0366-6964.2017.10.001

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (10): 1785-1795.doi: 10.11843/j.issn.0366-6964.2017.10.001

piRNA的形成及其在雌性动物中生物学功能研究进展

李春艳^1,2, 任春环², 刘秋月¹, 胡文萍¹, 王翔宇¹, 曹晓涵¹, 张子军², 狄冉^1*, 储明星^1*

1. 中国农业科学院北京畜牧兽医研究所, 农业部动物遗传育种与繁殖重点实验室, 北京 100193;
2. 安徽农业大学动物科技学院, 合肥 230036

收稿日期:2017-04-17 出版日期:2017-10-23 发布日期:2017-10-24
通讯作者: 狄冉,博士,副研究员,E-mail:dirangirl@163.com;储明星,博士,研究员,主要从事羊优异繁殖性状的分子机理研究,E-mail:mxchu@263.net
作者简介:李春艳(1992-),女,甘肃通渭人,硕士生,主要从事动物分子育种研究,E-mail:3010992163@qq.com
基金资助:
国家自然科学基金项目（31572371；31472078；31402041）；宁夏农林科学院科技创新先导资金项目（DWJLC-2016001）；中国农业科学院科技创新工程（ASTIP-IAS13）；国家肉羊产业技术体系专项（CARS-39）；中央级公益性科研院所基本科研业务费专项（2013ywf-zd-1；2015ywf-zd-2；2015ywf-zd-8）；内蒙古自治区科技重大专项；内蒙古自治区战略性新兴产业发展专项资金计划。

Research Progress on piRNA Formation and Function in Female Animals

LI Chun-yan^1,2, REN Chun-huan², LIU Qiu-yue¹, HU Wen-ping¹, WANG Xiang-yu¹, CAO Xiao-han¹, ZHANG Zi-jun², DI Ran^1*, CHU Ming-xing^1*

1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received:2017-04-17 Online:2017-10-23 Published:2017-10-24

摘要/Abstract

摘要：

piRNA是继siRNA和miRNA之后发现的一类新型非编码小RNA，其与Piwi蛋白协同作用参与piRNA通路，保护动物生殖细胞的遗传信息免受分子寄生虫如转座子的有害影响。近年来，人们对piRNA发生过程的相关蛋白及生物学作用做了研究，发现多种Piwi亚家族蛋白能与piRNA结合形成Piwi-piRNA复合体，通过表观遗传调控参与生殖细胞形成与发育、生殖干细胞分化、性别决定等生物学功能。本文主要围绕piRNA的形成及特点、Piwi-piRNA复合体发挥生物学作用的方式、piRNA在雌性动物中的生物学功能这3个方面对近期的研究进展进行综述，为深入探究piRNA调控雌性动物繁殖机制提供参考。

关键词: piRNA, Piwi蛋白, 表观遗传调控, 雌性动物

Abstract:

Following the discovery of siRNA and miRNA, a new class of small noncoding RNA, called piRNA, was found. piRNA can interact with Piwi subfamily proteins and they involve in piRNA pathways,which can protect genetic information of animal germ cells from the harmful effects of molecular parasites such as transposon. In recent years,piRNA biogenesis-related proteins and their biological effects were studied. It was found that a variety of Piwi subfamily proteins could bind to piRNA to form Piwi-piRNA complex. These complexes were involved in germ cells formation and development, germline stem cells differentiation, sex determination and other biological functions through the way of epigenetic regulation. In the present paper,we reviewed the research progress on the biogenesis and characteristics of piRNA,the ways of Piwi-piRNA complexes playing the biological roles and biological functions of piRNA in female animals, which will provide a reference for further research on regulatory mechanism of piRNA in female animal reproduction.

Key words: piRNA, Piwi protein, epigenetic regulation, female animals

中图分类号:

Q953
S813.1

李春艳, 任春环, 刘秋月, 胡文萍, 王翔宇, 曹晓涵, 张子军, 狄冉, 储明星. piRNA的形成及其在雌性动物中生物学功能研究进展[J]. 畜牧兽医学报, 2017, 48(10): 1785-1795.

LI Chun-yan, REN Chun-huan, LIU Qiu-yue, HU Wen-ping, WANG Xiang-yu, CAO Xiao-han, ZHANG Zi-jun, DI Ran, CHU Ming-xing. Research Progress on piRNA Formation and Function in Female Animals[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1785-1795.

参考文献

[1] LEE R C, FEINBAUM R L, AMBROS V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell, 1993, 75(5): 843-854.
[2] ARAVIN A, GAIDATZIS D, PFEFFER S, et al. A novel class of small RNAs bind to MILI protein in mouse testes[J]. Nature, 2006, 442(7099): 203-207.
[3] GRIVNA S T, BEYRET E, WANG Z, et al. A novel class of small RNAs in mouse spermatogenic cells[J]. Genes Dev, 2006, 20(13): 1709-1714.
[4] GONZALEZ J, QI H Y, LIU N, et al. Piwi is a key regulator of both somatic and germline stem cells in the Drosophila testis[J]. Cell Rep, 2015, 12(1): 150-161.
[5] HUANG Y, BAI J Y, REN H T. PiRNAs biogenesis and its functions[J]. Bioorg Khim, 2014, 40(3): 320-326.
[6] KIUCHI T, KOGA H, KAWAMOTO M, et al. A single female-specific piRNA is the primary determiner of sex in the silkworm[J]. Nature, 2014, 509(7502): 633-636.
[7] HUNTRISS J, LU J P, HEMMINGS K, et al. Isolation and expression of the human gametocyte-specific factor 1 gene (GTSF1) in fetal ovary, oocytes, and preimplantation embryos[J]. J Assist Reprod Genet, 2017, 34(1): 23-31.
[8] DIETRICH I, SHI X H, MCFARLANE M, et al. The antiviral RNAi response in vector and non-vector cells against orthobunyaviruses[J]. PLoS Negl Trop Dis, 2017, 11: e0005272.
[9] HUANG G, HU H, XUE X, et al. Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer[J]. Clin Transl Oncol, 2013, 15(7): 563-568.
[10] ASSUMPÇÃO C B, CALCAGNO D Q, ARAÚJO T M T, et al. The role of piRNA and its potential clinical implications in cancer[J]. Epigenomics, 2015, 7(6): 975-984.
[11] YAMANAKA S, SIOMI M C, SIOMI H. piRNA clusters and open chromatin structure[J]. Mob DNA, 2014, 5: 22.
[12] MOYANO M, STEFANI G. piRNA involvement in genome stability and human cancer[J]. J Hematol Oncol, 2015, 8: 38.
[13] GIRARD A, HANNON G J. Conserved themes in small-RNA-mediated transposon control[J]. Trends Cell Biol, 2008, 18(3): 136-148.
[14] WEICK E M, MISKA E A. piRNAs: from biogenesis to function[J]. Development (Cambridge, England), 2014, 141(18): 3458-3471.
[15] CZECH B, HANNON G J. One loop to rule them all: the Ping-Pong cycle and piRNA-guided silencing[J]. Trends Biochem Sci, 2016, 41(4): 324-337.
[16] LE THOMAS A, TÓTH K F, ARAVIN A A. To be or not to be a piRNA: genomic origin and processing of piRNAs[J]. Genome Biol, 2014, 15: 204.
[17] LUTEIJN M J, KETTING R F. Piwi-interacting RNAs: from generation to transgenerational epigenetics[J]. Nat Rev Genet, 2013, 14(8): 523-534.
[18] SAITO K, ISHIZU H, KOMAI M, et al. Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila[J]. Genes Dev, 2010, 24(22): 2493-2498.
[19] LAKSHMI S S, AGRAWAL S. piRNABank: a web resource on classified and clustered Piwi-interacting RNAs[J]. Nucleic Acids Res, 2008, 36(S1): D173-D177.
[20] ZHANG Y, WANG X H, KANG L. A k-mer scheme to predict piRNAs and characterize locust piRNAs[J]. Bioinformatics, 2011, 27(6): 771-776.
[21] ZANG P, SI X H, SKOGERBØ G, et al. piRBase: a web resource assisting piRNA functional study[J]. Database, 2014, 2014: bau110.
[22] 许甘霖, 齐绪峰, 蔡冬青. 依据“ping-pong”循环机制预测piRNA靶基因的搜索方法[J]. 基础医学与临床, 2017, 37(3): 399-401.
XU G L, QI X F, CAI D Q. The research method of predicting piRNA target genes by “ping-pong”mechanism[J]. Basic & Clinical Medicine, 2017, 37(3): 399-401. (in Chinese)
[23] LIN H, SPRADLING A C. A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary[J]. Development (Cambridge, England), 1997, 124(12): 2463-2476.
[24] SAITO K, NISHIDA K M, MORI T, et al. Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome[J]. Genes Dev, 2006, 20(16): 2214-2222.
[25] SAMJI T. Piwi, piRNAs, and germline stem cells: What's the link?[J]. Yale J Biol Med, 2009, 82(3): 121-124.
[26] HAN B W, ZAMORE P D. PiRNAs[J]. Curr Biol, 2014, 24(16): R730-R733.
[27] SASAKI T, SHIOHAMA A, MINOSHIMA S, et al. Identification of eight members of the Argonaute family in the human genome[J]. Genomics, 2003, 82(3): 323-330.
[28] KLATTENHOFF C, THEURKAUF W. Biogenesis and germline functions of piRNAs[J]. Development (Cambridge, England), 2008, 135(1): 3-9.
[29] TÓTH K F, PEZIC D, STUWE E, et al. The piRNA pathway guards the germline genome against transposable elements[C]//Advances in Experimental Medicine and Biology. Dordrecht: Springer, 2016, 886: 51-77.
[30] LE THOMAS, ROGERS A K, WEBSTER A, et al. Piwi induces piRNA-guided transcriptional silencing and establishment of a repressive chromatin state[J]. Genes Dev, 2013, 27(4): 390-399.
[31] DI GIACOMO M, COMAZZETTO S, SAINI H, et al. Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis[J]. Mol Cell, 2013, 50(4): 601-608.
[32] BUCKLEY B A, BURKHART K B, GU S G, et al. A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality[J]. Nature, 2012, 489(7416): 447-451.
[33] COX D N, CHAO A, LIN H. Piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells[J]. Development (Cambridge, England), 2000, 127(3): 503-514.
[34] WILCZYNSKA A, MINSHALL N, ARMISEN J, et al. Two Piwi proteins, Xiwi and Xili, are expressed in the Xenopus female germline[J]. RNA, 2009, 15(2): 337-345.
[35] HOUWING S, KAMMINGA L M, BEREZIKOV E, et al. A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish[J]. Cell, 2007, 129(1): 69-82.
[36] AKKOUCHE A, MUGAT B, BARCKMANN B, et al. Piwi is required during Drosophila embryogenesis to license dual-strand piRNA clusters for transposon repression in adult ovaries[J]. Mol Cell, 2017, 66(3): 411-419,e4.
[37] KIRINO Y, KIM N, DE PLANELL-SAGUER M, et al. Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability[J]. Nat Cell Biol, 2009, 11(5): 652-658.
[38] SAITO K, SIOMI M C. Small RNA-mediated quiescence of transposable elements in animals[J]. Dev Cell, 2010, 19(5): 687-697.
[39] KLATTENHOFF C, XI H L, LI C J, et al. The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters[J]. Cell, 2009, 138(6): 1137-1149.
[40] MALONE C D, BRENNECKE J, DUS M, et al. Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary[J]. Cell, 2009, 137(3): 522-535.
[41] MOHN F, SIENSKI G, HANDLER D, et al. The rhino-deadlock-cutoff complex licenses noncanonical transcription of dual-strand piRNA clusters in Drosophila[J]. Cell, 2014, 157(6): 1364-1379.
[42] LUO S, LU J. Silencing of transposable elements by piRNAs in Drosophila: an evolutionary perspective[J]. Genomics Proteomics Bioinformatics, 2017, 15(3): 164-176, doi: 10.1016/j.gpb.2017.01.006.
[43] 傅子文. 果蝇基因Twin在卵巢生殖干细胞系的维持和分化中的作用[D]. 北京: 清华大学, 2015.
FU Z W. Twin gene functions in the maintenance and differentiation of the ovarian germline stem cell lineage in Drosophila[D]. Beijing: Tsinghua University, 2015. (in Chinese)
[44] LESLIE M. The immune system's compact genomic counterpart[J]. Science, 2013, 339(6115): 25-27.
[45] GOU L T, DAI P, YANG J H, et al. Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis[J]. Cell Res, 2014, 24(6): 680-700.
[46] MARIE P P, RONSSERAY S, BOIVIN A. From embryo to adult: piRNA-mediated silencing throughout germline development in Drosophila[J]. G3 (Bethesda), 2017, 7(2): 505-516.
[47] YUAN J, LI M, WEI L, et al. Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes[J]. Cell Cycle, 2009, 8(20): 3384-3395.
[48] RICO-LEO E M, MORENO-MARIN N, GONZÁLEZ-RICO F J, et al. piRNA-associated proteins and retrotransposons are differentially expressed in murine testis and ovary of aryl hydrocarbon receptor deficient mice[J]. Open Biol, 2016, 6(12): 160-186.
[49] ARMISEN J, GILCHRIST M J, WILCZYNSKA A, et al. Abundant and dynamically expressed miRNAs, piRNAs, and other small RNAs in the vertebrate Xenopus tropicalis[J]. Genome Res, 2009, 19(10): 1766-1775.
[50] XU J, CHEN S Q, ZENG B S, et al. Bombyx mori p-element somatic inhibitor (BmPSI) is a key auxiliary factor for silkworm male sex determination[J]. PLoS Genet, 2017, 13(1): e1006576.
[51] SAKAI H, SUMITANI M, CHIKAMI Y, et al. Transgenic expression of the piRNA-resistant Masculinizer gene induces female-specific lethality and partial female-to-male sex reversal in the silkworm, Bombyx mori[J]. PLoS Genet, 2016, 12(8): e1006203.
[52] 高杰. 家蚕内源性piRNA促进家蚕piggyBac转座子的稳定性[D]. 重庆: 西南大学, 2015.
GAO J. Endogenous piRNA promote the stability of piggy Bac transposon in Bombyx mori[D]. Chongqing: Southwest University, 2015.(in Chinese)
[53] PATIL A A, TATSUKE T, MON H, et al. Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell[J]. Biochem Biophys Res Commun, 2017, 490(2): 134-140, doi: 10.1016/j.bbrc.2017.06.008.
[54] 谢逸菲. 桔小实蝇piRNA通路相关基因tudor及海藻糖酶基因对卵巢发育影响的研究[D]. 重庆: 西南大学, 2015.
XIE Y F. The influence of piRNA pathway related gene tudor and trehalase gene on ovary development in Bactrocera dorsalis[D]. Chongqing: Southwest University, 2015. (in Chinese)
[55] DAI X Y, SHU Y Q, LOU Q Y, et al. Tdrd12 is essential for germ cell development and maintenance in Zebrafish[J]. Int J Mol Sci, 2017, 18(6): 1127.
[56] 陈蓉, 秦玉蓉, 陈国宏, 等. 地方鸡种小分子RNA在性腺中表达形式的初步研究[J]. 畜牧与兽医, 2009, 41(10): 30-33.
CHEN R, QIN Y R, CHEN G H, et al. The preliminry study of micromolecule RNA expression form in indigenous chicken breeds, gonads[J]. Animal Husbandry & Veterinary Medicine, 2009, 41(10): 30-33. (in Chinese)
[57] 周毅. 多倍体鲫鲤Piwi和piRNA的表达与育性的相关性研究[D]. 长沙: 湖南师范大学, 2012.
ZHOU Y. Piwi and piRNA expression and their relationships with fertility of ploidy Cyprinids[D]. Changsha: Hunan Normal University, 2012. (in Chinese)
[58] KOWALCZYKIEWICZ D, PAWLAK P, LECHNIAK D, et al. Altered expression of porcine Piwi genes and piRNA during development[J]. PLoS One, 2012, 7(8): e43816.
[59] WILLIAMS Z, MOROZOV P, MIHAILOVIC A, et al. Discovery and characterization of piRNAs in the human fetal ovary[J]. Cell Rep, 2015, 13(4): 854-863.
[60] CHENG J, GUO J M, XIAO B X, et al. piRNA, the new non-coding RNA, is aberrantly expressed in human cancer cells[J]. Clin Chim Acta, 2011, 412(17-18): 1621-1625.
[61] YAN H, WU Q L, SUN C Y, et al. piRNA-823 contributes to tumorigenesis by regulating de novo DNA methylation and angiogenesis in multiple myeloma[J]. Leukemia, 2015, 29(1): 196-206.

piRNA的形成及其在雌性动物中生物学功能研究进展

Research Progress on piRNA Formation and Function in Female Animals

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	殷实, 秦文昌, 王斌, 周婧雯, 杨柳青, 李键. 三个阶段牦牛睾丸发育过程中piRNA的鉴定及分析研究[J]. 畜牧兽医学报, 2019, 50(11): 2235-2243.
[2]	孙武, 罗静, 李发弟, 李万宏, 乐祥鹏. 基于转录组学筛选与哺乳动物睾丸发育相关基因及调控网络的研究进展[J]. 畜牧兽医学报, 2018, 49(9): 1810-1817.
[3]	孙武, 罗静, 李发弟, 李万宏, 乐祥鹏. 基于转录组学筛选与哺乳动物睾丸发育相关基因及调控网络的研究进展[J]. 畜牧兽医学报, 2018, 49(9): 1810-1817.
[4]	李春艳, 任春环, 刘秋月, 胡文萍, 王翔宇, 曹晓涵, 张子军, 狄冉, 储明星. piRNA的形成及其在雌性动物中生物学功能研究进展[J]. 畜牧兽医学报, 2017, 48(10): 1785-1795.
[5]	戴爱琴，常国斌，陈蓉，张颖，阴彦辉，马腾，李建超，陈国宏. 鸡Piwi蛋白的亚细胞定位研究[J]. 畜牧兽医学报, 2013, 44(1): 15-22.
[6]	戴爱琴，常国斌，陈蓉，张颖，阴彦辉，马腾，李建超，陈国宏. 鸡Piwi蛋白的亚细胞定位研究[J]. 畜牧兽医学报, 2013, 44(1): 15-22.
[7]	张颖;常国斌;陈蓉;戴爱琴;栾德琴;李建超;马腾;华登科;陈国宏. 鸡pilRNAs分子的克隆及表达规律分析[J]. 畜牧兽医学报, 2012, 43(6): 857-866.
[8]	张颖;常国斌;陈蓉;戴爱琴;栾德琴;李建超;马腾;华登科;陈国宏. 鸡pilRNAs分子的克隆及表达规律分析[J]. 畜牧兽医学报, 2012, 43(6): 857-866.
[9]	刘雪青，杨浩，王建刚，曹斌云. piRNA的发生和相关蛋白的研究进展[J]. 畜牧兽医学报, 2012, 43(12): 1855-1864.
[10]	刘雪青，杨浩，王建刚，曹斌云. piRNA的发生和相关蛋白的研究进展[J]. 畜牧兽医学报, 2012, 43(12): 1855-1864.