黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响

doi:10.11843/j.issn.0366-6964.2017.06.018

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (6): 1128-1139.doi: 10.11843/j.issn.0366-6964.2017.06.018

黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响

靳二辉¹, 陈耀星², 周金星¹, 李升和¹, 任曼¹, 胡倩倩¹, 金光明^1*

1. 安徽科技学院动物科学学院, 凤阳 233100;
2. 中国农业大学动物医学院, 北京 100193

收稿日期:2016-09-22 出版日期:2017-06-23 发布日期:2017-06-15
通讯作者: 金光明,教授,E-mail:jingm@ahstu.edu.cn
作者简介:靳二辉(1980-),男,河南汝州人,副教授,博士,主要从事动物解剖及组织胚胎学研究,Tel:0550-6732040,E-mail:jineh@ahstu.edu.cn
基金资助:
国家自然科学基金（31402154）；国家星火计划面上项目（2015GA710015）；北京农学院兽医学（中兽医）北京市重点实验室开放课题（kf2016035）；安徽省农业科技成果转化项目（1504032001）；安徽省科技攻关项目（1301042102）；安徽省高等学校自然科学研究重点项目（KJ2016A823；KJ2017A505）；安徽省第七批“115”产业创新团队项目（皖人才[2014]4号）；安徽科技学院重点建设学科项目（AKZDXK2015A04）

The Effect of Compound Chinese Herbal Medicine Preparation of Astragalus, Wolfberry and Honeysuckle Flower on Immune Organ Development and Immune Functions of Broiler

JIN Er-hui¹, CHEN Yao-xing², ZHOU Jin-xing¹, LI Sheng-he¹, REN Man¹, HU Qian-qian¹, JIN Guang-ming^1*

1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China;
2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received:2016-09-22 Online:2017-06-23 Published:2017-06-15

摘要/Abstract

摘要：

为了研究由黄芪、枸杞、金银花等药材组成的复方中草药制剂对肉鸡免疫器官发育及免疫功能的影响，将1日龄健康AA肉鸡1 000羽随机分为对照组和试验Ⅰ、Ⅱ、Ⅲ组，每组5个重复，每个重复50羽，对照组饲喂基础日粮，试验Ⅰ、Ⅱ和Ⅲ组分别在基础日粮中添加0.5%、1.0%和1.5%的复方中草药制剂，试验期42 d。结果显示，在21日龄时，试验Ⅱ组肉鸡法氏囊指数、血清IgG和IgM含量以及NDV抗体和IL-4水平均显著高于对照组（P<0.05），试验Ⅲ组脾质量和器官指数以及血清IL-2和IFN-γ水平均显著高于对照组（P<0.05），试验Ⅰ组肉鸡血清IgG含量和NDV抗体水平均显著高于对照组（P<0.05）；在42日龄时，试验Ⅱ组肉鸡胸腺和法氏囊质量与器官指数、血清IgG和IgM含量以及NDV抗体和IL-4水平均显著高于对照组（P<0.05），试验Ⅲ组肉鸡脾质量与器官指数、血清IgG和IgM含量以及IL-2、IL-4和IFN-γ水平均显著高于对照组（P<0.05），试验Ⅰ组肉鸡血清IgG和IgM含量以及NDV抗体水平均显著高于对照组（P<0.05）。显微观察可见，在21日龄时，试验Ⅱ组肉鸡法氏囊组织结构清晰，法氏囊小结面积显著大于对照组（P<0.05），小结内淋巴细胞数量较多，排列紧密。在42日龄时，试验Ⅱ组肉鸡胸腺皮质厚度显著大于对照组（P<0.05），法氏囊小结面积显著大于对照组（P<0.05）；试验Ⅲ组肉鸡胸腺皮质厚度显著大于对照组（P<0.05），脾小结和脾动脉周围淋巴鞘小结面积显著大于对照组（P<0.05），脾小结数量也明显较多；试验Ⅰ组肉鸡脾小结面积也明显大于对照组（P<0.05）。结果表明，日粮中添加不同剂量黄芪、枸杞、金银花等复方中草药制剂对肉鸡免疫器官发育和免疫功能可产生不同程度的促进作用，其中以添加1.0%复方中草药制剂效果最好。

关键词: 复方中草药, 肉鸡, 免疫器官, 免疫功能

Abstract:

To study the effect of compound Chinese herbal composed of Astragalus, Chinese wolfberry, honeysuckle and other herbs on immune organ development and immune function of broilers. A total of 1 000 one-day-old Avian broilers (AA broilers) were randomly divided into control group and experimental groupsⅠ, Ⅱ and Ⅲ, each group included 5 pens (50 birds per pen). Control group was fed with the basal diet and experimental groupsⅠ, Ⅱ and Ⅲ were fed with the basal diet supplemented with 0.5%, 1.0% and 1.5% compound Chinese herbal for 42 days, respectively. Results showed: compared with control group, the index of bursa of Fabricius, serum IgG and IgM concentration, serum NDV antibody and IL-4 levels were significantly increased in experimental group Ⅱ(P<0.05); the weight and organ index of thymus, the levels of serum IL-2 and IFN-γ were significantly increased in experimental group Ⅲ(P<0.05); the serum IgG concentration and NDV antibody level were significantly increased in experimental groupⅠof broilers (P<0.05). At 42 days of age, the weight and organ index of thymus and bursa, the serum IgG and IgM concentration, the serum NDV antibody and IL-4 levels were significantly increased in experimental groupⅡ (P<0.05); the weight and organ index of spleen, the levels of serum IL-2,IL-4 and IFN-γ were significantly increased in experimental group Ⅲ(P<0.05); the serum IgG and IgM concentration, the serum NDV antibody level were significantly increased in experimental groupⅠcompared with control group of broilers (P<0.05). Microscopic observation has also showed: At 21 days of age, the structure of bursa of Fabricius was clear, the area of bursa of Fabricius node was obviously increased (P<0.05), in which the numbers of lymphocytes were also increased and tight arranged in experimental group Ⅱ of broilers. At 42 days of age, the thymic cortex were thicker (P<0.05), and the area of bursa of Fabricius node was obviously increased in experimental group Ⅱ of broilers (P<0.05); the thymic cortex were also thicker (P<0.05), the area of peripheral lymphatic sheath and spleen node were significantly increased (P<0.05), and the number of spleen node were increased in experimental group Ⅲ of broilers; the area of spleen node in the experiment group Ⅰ was significantly higher than that of the control group (P<0.05). The results have indicated that supplementation of different doses of compound Chinese herbal medicine preparation of Astragalus wolfberry and honeysuckle can improve immune organ development and immune function of broilers to some extent, in which supplementation of 1.0% compound Chinese herbal preparation have the best effect.

Key words: compound Chinese herbal, broiler, immune organ, immune function

中图分类号:

S853.7

靳二辉, 陈耀星, 周金星, 李升和, 任曼, 胡倩倩, 金光明. 黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响[J]. 畜牧兽医学报, 2017, 48(6): 1128-1139.

JIN Er-hui, CHEN Yao-xing, ZHOU Jin-xing, LI Sheng-he, REN Man, HU Qian-qian, JIN Guang-ming. The Effect of Compound Chinese Herbal Medicine Preparation of Astragalus, Wolfberry and Honeysuckle Flower on Immune Organ Development and Immune Functions of Broiler[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1128-1139.

参考文献

[1] 陈张华, 邓惠丹, 邓俊良, 等. 复方中草药"猪康散"对仔猪生产性能及细胞免疫功能的影响[J]. 中国兽医学报, 2012, 32(6):898-901.
CHEN Z H, DENG H D, DENG J L, et al. Effect of Zhukangsan on growth performance and cellular immune function of in piglets[J]. Chinese Journal of Veterinary Science, 2012, 32(6):898-901. (in Chinese)
[2] 王福传, 方昌阁, 张玉换, 等. 复方中草药免疫增强剂对蛋鸡免疫效果的研究[J]. 畜牧兽医学报, 2006, 37(2):187-192.
WANG F C, FANG C G, ZHANG Y H, et al. Effects of compound Chinese herbal immune synergist on immunity of egg-laying chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2006, 37(2):187-192. (in Chinese)
[3] 田树海, 曹洪战, 范苗, 等. 复方中草药添加剂对育肥猪生产性能和血液指标的影响[J]. 畜牧与兽医, 2010, 42(2):37-40.
TIAN S H, CAO H Z, FAN M, et al. Effect of compound Chinese herbal medicine additive on performance and blood index of finishing pigs[J]. Animal Husbandry & Veterinary Medicine, 2010, 42(2):37-40. (in Chinese)
[4] 李亮, 龚道清, 张军, 等. 复方中草药饲料添加剂对黄羽肉鸡免疫性能的影响[J]. 中国畜牧兽医, 2008, 35(1):26-28.
LI L, GONG D Q, ZHANG J, et al. Effect of Chinese herb medicine feed additive on immune performance of Chinese yellow-feathered chicken[J]. China Animal Husbandry & Veterinary Medicine, 2008, 35(1):26-28. (in Chinese)
[5] MARCIN?ÁK S, CABADAJ R, POPELKA P, et al. Antioxidative effect of oregano supplemented to broilers on oxidative stability of poultry meat[J]. Slov Vet Res, 2008, 45(2):61-66.
[6] WANG L, PIAO X L, KIM S W, et al. Effects of Forsythia suspensa extract on growth performance, nutrient digestibility, and antioxidant activities in broiler chickens under high ambient temperature[J]. Poul Sci, 2008, 87(7):1287-1294.
[7] 阴天榜, 刘兴友. 家禽免疫学[M]. 北京:中国农业科技出版社, 1999:194-201.
YIN T B, LIU X Y. Poultry immunology[M]. Beijing:China Agriculture Scientech Press, 1999:194-201. (in Chinese)
[8] RIVAS A L, FABRICANT J. Indications of immunodepression in chickens infected with various strains of Marek's disease virus[J]. Avian Dis, 1988, 32(1):1-8.
[9] 苏记良, 梁祖满, 罗秋兰, 等. 复方中草药对黄羽肉鸡生长性能及免疫功能的影响[J]. 饲料研究, 2016(10):13-16, 27.
SU J L, LIANG Z M, LUO Q L, et al. Effects of compound Chinese herbal medicine on growth performance and immune function of Huang Yu broilers[J]. Feed Research, 2016(10):13-16, 27. (in Chinese)
[10] 俞景林. 中草药添加剂对蛋雏鸡生长性能及免疫器官指数的影响研究[J]. 浙江畜牧兽医, 2013, 38(5):3-5.
YU J L. Effects of Chinese herbal medicine additives on growth performance and immune organ index of laying chicks[J]. Zhejiang Journal Animal Science and Veterinary Medicine, 2013, 38(5):3-5. (in Chinese)
[11] 赵红梅, 梁明振. 超微中草药添加剂对断奶仔猪免疫器官指数影响的研究[J]. 黑龙江畜牧兽医, 2014(4):138-139.
ZHAO H M, LIANG M Z. Study on the effect of Chinese herbal medicine additives on immune organ index of weaned piglets[J]. Heilongjiang Animal Science and Veterinary Medicine, 2014(4):138-139. (in Chinese)
[12] 陈国顺, 陈胜, 随亚楠. 中草药饲料添加剂对合作猪肠道消化酶活性和消化能力的影响[J]. 山东农业大学学报:自然科学版, 2014, 45(4):529-535.
CHEN G S, CHEN S, SUI Y N. Influence of Chinese herbal feed additives on wild pigs' activity of digestive enzyme in intestinal tract and ability to digest[J]. Journal of Shandong Agricultural University:Natural Science Edition, 2014, 45(4):529-535. (in Chinese)
[13] 甘辉群, 刘明生, 陈光明, 等. 复方黄芪制剂对雏鸡免疫机能的影响[J]. 中国兽药杂志, 2014, 48(12):27-31.
GAN H Q, LIU M S, CHEN G M, et al. The effects of compound Astragalus preparations on the immunity function in chickens[J]. Chinese Journal of Veterinary Drug, 2014, 48(12):27-31. (in Chinese)
[14] 王福传, 韩一超, 赵洪恩, 等. 复方中草药免疫增强剂对鸡免疫器官组织形态学影响的研究[J]. 中国预防兽医学报, 2001, 23(6):419-421.
WANG F C, HAN Y C, ZHAO H E, et al. The effect of compound Chinese herbal on chicken immune organs and tectology[J]. Chinese Journal of Preventive Veterinary Medicine, 2001, 23(6):419-421. (in Chinese)
[15] 李艳华, 闫清波, 于康震, 等. 复方中药对禽流感病毒的防治研究[J]. 中国农业科学, 2008, 41(5):1511-1518.
LI Y H, YAN Q B, YU K Z, et al. Study of the compound Chinese medicine against the anti-avian influenza virus[J]. Scientia Agricultural Sinica, 2008, 41(5):1511-1518. (in Chinese)
[16] 高杨, 王洪芳, 陈辉, 等. 饲粮添加黄芪多糖对蛋鸡免疫功能及肠道菌群的影响[J]. 动物营养学报, 2011, 23(3):447-451.
GAO Y, WANG H F, CHEN H, et al. Effects of dietary Astragalus Polysaccharides on immune function and intestinal flora in laying hens[J]. Chinese Journal of Animal Nutrition, 2011, 23(3):447-451. (in Chinese)
[17] 孙晓雨, 崔子寅, 张明亮, 等. 枸杞多糖和茯苓多糖对免疫抑制小鼠免疫增强及对肠道黏膜的免疫调节作用[J]. 中国兽医学报, 2015, 35(3):450-455.
SUN X Y, CUI Z Y, ZHANG M L, et al. Effect of Lycium barbarum polysaccharides and Pachymaran on immune function and intestinal mucosal system in immunosuppressive mice[J]. Chinese Journal of Veterinary Science, 2015, 35(3):450-455. (in Chinese)
[18] 程鹏, 白云静, 于晓红, 等. 甘草多糖提取工艺的研究及其对小鼠免疫口蹄疫疫苗后细胞免疫的影响[J]. 北京农学院学报, 2015, 30(3):30-34.
CHENG P, BAI Y J, YU X H, et al. Extraction process of Glycyrrhiza glabra polysaccharides and its cellular immune responses on the mice after immunization FMD vaccine[J]. Journal of Beijing University of Agriculture, 2015, 30(3):30-34. (in Chinese)
[19] XIE D, WANG Z, CAO J, et al. Effects of monochromatic light on proliferation response of Splencyte in broilers[J]. Anat Histol Embryol, 2008, 37(5):332-337.
[20] WINTER H, VAN DEN ENGEL N K, RÜTTINGER D, et al. Therapeutic T cells induce tumor-directed chemotaxis of innate immune cells through tumor-specific secretion of chemokines and stimulation of B16BL6 melanoma to secrete chemokines[J]. J Transl Med, 2007, 5(1):56.
[21] O'GARRA A, MURPHY K. Role of cytokines in determining T-lymphocyte function[J]. Curr Opin Immunol, 1994, 6(3):458-466.
[22] CHENG J J, LI Q Y, SHI W Y, et al. Effects of Huangqi Maxingshigan decoction on infectious laryngotracheitis in chickens[J]. Ital J Anim Sci, 2011, 10(2):124-130.
[23] REN Z H, NA L X, XU Y M, et al. Dietary supplementation with lacto-wolfberry enhances the immune response and reduces pathogenesis to influenza infection in mice[J]. J Nutr, 2012, 142(8):1596-1602.
[24] KATAYAMA M, FUKUDA T, OKAMURA T, et al. Effect of dietary addition of seaweed and licorice on the immune performance of pigs[J]. Anim Sci J, 2011, 82(2):274-281.
[25] 杨润霞, 吴润, 刘磊, 等. 三种中药复方制剂对雏鸡免疫器官发育及免疫功能的影响[J]. 甘肃农业大学学报, 2015, 50(4):28-33.
YANG R X, WU R, LIU L, et al. Effects of three traditional Chinese medicine prescriptions on the immune organ development and immune function of chicken[J]. Journal of Gansu Agricultural University, 2015, 50(4):28-33. (in Chinese)
[26] CHEN X, LIN H Z, JIANG S G, et al. Dietary supplementation of honeysuckle improves the growth, survival and immunity of Penaeus monodon[J]. Fish Shellfish Immunol, 2013, 35(1):161-169.
[27] LIU C, WANG G Q, CHEN G F, et al. Huangqi decoction inhibits apoptosis and fibrosis, but promotes Kupffer cell activation in dimethylnitrosamine-induced rat liver fibrosis[J]. BMC Complement Altern Med, 2012, 12(1):51.
[28] 胡永灵, 叶世莉, 罗佳捷. 中草药制剂对热应激奶牛泌乳性能、抗氧化能力及免疫功能的影响[J]. 草业学报, 2015, 24(1):132-140.
HU Y L, YE S L, LUO J J. Effect of Chinese herbal medicine on milk production, antioxidant capacity and immunity of dairy cows[J]. Acta Prataculturae Sinica, 2015, 24(1):132-140. (in Chinese)

黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响

The Effect of Compound Chinese Herbal Medicine Preparation of Astragalus, Wolfberry and Honeysuckle Flower on Immune Organ Development and Immune Functions of Broiler

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[14]	杜保华, 陈思, 张相德, 何宁, 杨欣, 呙于明, 姚军虎, 杨小军. 肉鸡皮大麦表观代谢能预测模型研究[J]. 畜牧兽医学报, 2018, 49(10): 2180-2190.
[15]	张敏, 王杰, 孙艳发, 钟礼鹏, 李焰, 许卫华, 李虹仪, 张茂, 黄其春. 肉鸡肌肉与脂肪组织基因组差异剪接基因分析[J]. 畜牧兽医学报, 2018, 49(10): 2124-2132.