PCV2人工感染对猪肺泡巨噬细胞干扰素信号通路的调控研究

doi:10.11843/j.issn.0366-6964.2016.03.023

摘要/Abstract

摘要：

通过建立PCV2亚临床感染模型，探究感染仔猪肺泡巨噬细胞中干扰素的变化及其调控，为PCV2复制和发病机制研究提供补充。30日龄猪圆环病毒2型（PCV2）和猪繁殖与呼吸综合征病毒（PRRSV）抗原和抗体均为阴性的仔猪15头，随机分成PCV2阴性对照0 d组、PCV2感染14 d组和PCV2感染28 d组。感染后荧光定量PCR方法检测肺泡巨噬细胞中干扰素、模式识别受体和模式识别受体接头分子mRNA水平。结果显示，IFN-β和IFN-γ mRNA转录水平在14和28 d均显著高于对照组(P＜0.05)；模式识别受体RIG-1、MDA-5、TLR4和TLR9 mRNA转录水平在14和28 d显著升高（P＜0.05）；DAI mRNA转录水平在14 d显著升高（P＜0.05），但28 d迅速下降； TLR3、TLR7和TLR8 mRNA转录水平无明显变化。接头分子MAVS、MyD88、IRF3和IRF7 mRNA转录水平在14和28 d均显著高于对照组（P＜0.05）。以上结果表明，PCV2亚临床感染仔猪后导致肺泡巨噬细胞内IFN-β和IFN-γ的表达量上调，其上调和PCV2激活的TLR4/TLR9/MyD88和RIG-1/MDA-5/DAI/MAVS/IRF3信号通路有关。

关键词: PCV2, 仔猪, 肺泡巨噬细胞, 干扰素, 模式识别受体

Abstract:

To understand the mechanism of PCV2 replication and pathogenesis，change and regulation of interferon in alveolar macrophages of piglets subclinically infected with procine circovirus type 2(PCV2) were studied.Fifteen 30-day-old piglets were selected as experiment animals，which were free of PCV2 and porcine reproductive and respiratory syndrome virus(PRRSV) antibody and antigen.The piglets were divided into three groups：control group(0 dpi)，PCV2 infection group(14 dpi) and PCV2 infection group(28 dpi).After infection，the mRNA transcription of interferon，pattern recognition receptors(PRRs) and pathogen associated molecular proteins(PAMPs) in AMs were detected by real-time PCR.The results showed that the mRNA levels of IFN-β and IFN-γ were significantly higher at 14 dpi and 28 dpi(P＜0.05)；the mRNA expression of RIG-1，MDA-5，TLR4 and TLR9 were significantly increased at 14 dpi and 28 dpi(P＜0.05)；the mRNA level of DAI was significantly elevated at 14 dpi(P＜0.05)，and then decreased at 28 dpi；while there were no significant change in mRNA expression of TLR3，TLR7 and TLR8.Compared with control，the mRNA transcription of MAVS，MyD88，IRF3 and IRF7 were significantly increased at 14 dpi and 28 dpi(P＜0.05).These results demonstrate that the up-regulations of IFN-β and IFN-γ in alveolar macrophages of PCV2 subclinical infection are relative to TLR4/TLR9/MyD88 and RIG-1/MDA-5/DAI/MAVS/IRF3 signaling pathways.

Key words: procine circovirus type2, piglet, alveolar macrophages, interferon, pattern recognition receptors

中图分类号:

S852.3

赵其岭，张新晨，陈萌萌，孙嘉瑞，鲍恩东，张书霞，吕英军. PCV2人工感染对猪肺泡巨噬细胞干扰素信号通路的调控研究[J]. 畜牧兽医学报, 2016, 47(3): 587-594.

ZHAO Qi-ling，ZHANG Xin-chen，CHEN Meng-meng，SUN Jia-rui，BAO En-dong，ZHANG Shu-xia，Lü Ying-jun. Regulation of Interferon Signaling Pathway in Alveolar Macrophages of Piglets Infected with Procine Circovirus Type 2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 587-594.

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