GnRH主动免疫雌性大鼠调控机制研究

doi:10.11843/j.issn.0366-6964.2016.03.011

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (3): 502-508.doi: 10.11843/j.issn.0366-6964.2016.03.011

GnRH主动免疫雌性大鼠调控机制研究

曹晓涵¹，韩兴发¹，杜小刚¹，陈志渝¹，孟风艳¹，储明星²，曾宪垠^1*

（1.四川农业大学生命科学学院，雅安 625014；2.中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与种质创新重点实验室，北京 100193）

收稿日期:2015-05-27 出版日期:2016-03-23 发布日期:2016-03-21
通讯作者: 曾宪垠，研究员，主要从事动物生殖免疫调控研究，E-mail：xyzeng1966@163.com
作者简介:曹晓涵(1984-)，女，四川成都人，讲师，博士，主要从事生殖免疫调控研究，E-mail：caoxiaohan2007@hotmail.com
基金资助:
四川省科技厅国际合作项目(2012HH0013)；四川农业大学双支计划(00770107)

Regulatory Mechanisms of Active Immunization against GnRH in Female Rats and Its Mechanism

CAO Xiao-han¹，HAN Xing-fa¹，DU Xiao-gang¹，CHEN Zhi-yu¹，MENG Feng-yan¹，CHU Ming-xing²，ZENG Xian-yin^1*

（1.College of Life Science，Sichuan Agricultural University，Ya’an 625014，China；2.Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture，Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193，China）

Received:2015-05-27 Online:2016-03-23 Published:2016-03-21

摘要/Abstract

摘要：

旨在探讨GnRH主动免疫雌性SD大鼠的调控机制。24只雌性SD大鼠随机分为免疫组、卵巢切除组和空白对照组（n=8），免疫组于12周龄注射疫苗，4周后加免；卵巢切除组11周龄外科手术摘除卵巢；空白对照组不作任何处理。使用放射免疫分析法测定血清激素E₂、P₄、FSH和LH含量，荧光定量PCR分析各基因mRNA表达情况。结果显示，主动免疫显著降低血清E₂、P₄、FSH和LH含量，至56周处死时，E₂和LH含量有微弱上升，但4种激素含量均显著低于空白对照组（P＜0.05）。与空白对照组相比，主动免疫显著下调下丘脑GPR54、KiSS-1、GnRH和ER-α mRNA，垂体GnRH-R、FSH-β和LH-β mRNA以及卵巢FSHR、LHR和ER-α mRNA的表达水平（P＜0.05）。结果表明，GnRH主动免疫能显著抑制雌性SD大鼠性腺发育，降低血清性激素含量，通过下丘脑ER-α mRNA-GPR54/KiSS-1通路保持动物较长时间去势；但GnRH主动免疫造成的动物去势存在恢复的可能。

关键词: GnRH, 主动免疫, 调控, 机制, 雌性大鼠

Abstract:

To explore the regulatory mechanisms of active immunization against GnRH in female SD rats.24 adult female SD rats were allocated to 3 groups of 8 animals each randomly.8 rats were immunized against GnRH at 12 week of age，4 weeks later an equal dose booster was administered.8 rats were surgically ovarictomy at 11 week of age while 8 intact rats without any treatments.Blood samples were collected at day 0(0 wpv) until just before sacrificed at 56 wpv.Serum levels of FSH，LH，E₂and P₄ were determined using RIA.After sacrificed，hypothalamus and pituitary were removed，the mRNA expressions of reproduction-related genes were determined by real-time fluorescence quantitative PCR(RT-PCR)；Ovaries were excised，adipose tissues were removed，then the mRNA expressions for reproduction-related genes were determined by RT-PCR.After immunization twice against GnRH，the concentrations of E₂，P₄，FSH and LH in immunized rats showed a significantly lower than that in Control group (P＜0.05).Moreover，active immunization against GnRH significantly down-regulated the mRNA levels of GnRH，GPR54，KiSS1and ER-α in the hypothalamus，GnRH-R，FSH-β and LH-β in the pituitary(P＜0.05)，as well as FSHR，LHR and ER-α in ovaries(P＜0.05).This study indicated GnRH had excellent immunogenic and was a good alternative of surgical castration.Active immunization against GnRH cause animals in a long-term infertility or sterility by the feedback loop of ER-α- GPR54/KiSS-1，and the fertility of immunocastrated rats show a tendancy to recover.

Key words: GnRH, active immunization, regulatory, mechanisms, female rat

中图分类号:

S852.4

曹晓涵，韩兴发，杜小刚，陈志渝，孟风艳，储明星，曾宪垠. GnRH主动免疫雌性大鼠调控机制研究[J]. 畜牧兽医学报, 2016, 47(3): 502-508.

CAO Xiao-han，HAN Xing-fa，DU Xiao-gang，CHEN Zhi-yu，MENG Feng-yan，CHU Ming-xing，ZENG Xian-yin. Regulatory Mechanisms of Active Immunization against GnRH in Female Rats and Its Mechanism[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 502-508.

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GnRH主动免疫雌性大鼠调控机制研究

Regulatory Mechanisms of Active Immunization against GnRH in Female Rats and Its Mechanism

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