基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因

doi:10.11843/j.issn.0366-6964.2016.01.004

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (1): 25-33.doi: 10.11843/j.issn.0366-6964.2016.01.004

基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因

束婧婷^#，章明^#，宋迟，单艳菊，徐文娟，宋卫涛，李慧芳^*

（江苏省家禽科学研究所，扬州 225125）

收稿日期:2015-01-22 出版日期:2016-01-23 发布日期:2016-01-19
通讯作者: 李慧芳，研究员，E-mail：lhfxf_002@aliyun.com
作者简介:束婧婷(1980-)，女，江苏宿迁人，博士，副研究员，主要从事家禽遗传育种研究，Tel：0514-85599018，E-mail：shujingting@163.com；章明（1978-），男，江苏姜堰人，硕士，副研究员，主要从事家禽遗传育种研究，E-mail：6259513@qq.com。二人共为第一作者
基金资助:
国家自然科学基金（31301967）；现代农业产业技术体系建设专项资金（CARS-42-G03）；江苏省自然科学基金（BK2012268）；江苏省农业科技自主创新资金项目（cx（15）1009）

Identification of Differentially Expressed Genes for Myofiber Characteristics in Soleus Muscles between Qingyuan Partridge Chickens and Cobb Broilers

SHU Jing-ting^#，ZHANG Ming^#，SONG Chi，SHAN Yan-ju，XU Wen-juan，SONG Wei-tao，LI Hui-fang^*

(Jiangsu Institute of Poultry Science，Yangzhou 225125，China)

Received:2015-01-22 Online:2016-01-23 Published:2016-01-19

摘要/Abstract

摘要：

对生长速度长期的高压选择导致了快大型肉鸡与优质型地方鸡显著的表型差异，本研究旨在研究这种表型差异的分子遗传机理。采用Agilent鸡全基因组表达谱芯片对达到性成熟的优质型清远麻鸡（112 d）和快大型科宝肉鸡(42 d)比目鱼肌中与肌纤维发育和类型组成相关的候选基因及信号通路进行系统筛查。结果，芯片分析共筛选到差异倍数在2倍及以上的基因1 318个，以科宝肉鸡作为参照，清远麻鸡中上调基因501个，下调基因817个，主要涉及到肌肉发育、能量代谢、脂质代谢等生物学过程。基于KEGG Pathway分析发现，差异基因除了参与肌纤维发育和分化相关信号通路（如Hedgehog信号通路和Ca²⁺信号通路）外，Wnt信号通路，mTOR信号通路，MAPK信号通路，ErbB信号通路、JAK-STAT信号通路等一些跟能量代谢相关的信号通路也被富集为显著的信号通路。与能量代谢相关的信号通路和与肌肉发育相关的信号通路相互作用形成一个调控网络从而影响肌纤维的发育，筛选出了20个在肌纤维生长发育及分化过程中可能具有重要影响的候选基因，但这些差异表达基因在肌纤维的发育和分化过程中的作用尚待深入研究。

关键词: 肌纤维性状, 表达谱芯片, 差异表达基因, 清远麻鸡, 科宝肉鸡

Abstract:

In the modern chicken industry，fast-growing broilers have undergone strong artificial selection for muscle growth，which has led to remarkable phenotypic variations compared with slow-growing chickens.The present study was designed to explore the molecular mechanism underlying these phenotypes differences.Agilent cDNA microarray analyses were conducted to determine gene expression profiles of soleus muscle sampled at sexual maturity age of Qingyuan Partridge chickens (QY，112 d，a slow-growing Chinese breed with high meat quality) and Cobb broilers (CB，42 d，a commercial fast-growing broiler line).A systematic identification of candidate genes and new pathways related to myofiber development and composition in soleus muscles between the 2 breeds were analyzed.1 318 genes with at least 2-fold differences were identified in soleus muscles of QY and CB chickens.When slow-growing QY chickens were compared with fast-growing CB chickens，the expression of 501 genes were up-regulated，and 817 genes were down-regulated.These genes mainly were related to muscle development，energy metabolism or lipid metabolism processes.In addition，based on KEGG pathway analysis，it was found that in addition to pathways affecting myofiber development and differentiation (pathways for Hedgehog & Calcium signaling)，energy metabolism signaling pathways (Wnt signaling pathway，mTOR signaling pathway，MAPK signaling pathway，ErbB signaling pathway and JAK-STAT signaling pathway) were also enriched.Our data indicates that energy and muscle metabolism pathways might form a network，which influence the development of myofibers，20 genes were potentially related to myofiber development and composition.However，large scale analyses are still required to validate the role of the genes identified and ultimately to find molecular markers that can be used for selection or to optimize rearing practices.

Key words: myofiber characteristics, microarray, differentially expressed gene, Qingyuan Partridge chicken, Cobb broiler

中图分类号:

S813.2

束婧婷，章明，宋迟，单艳菊，徐文娟，宋卫涛，李慧芳. 基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因[J]. 畜牧兽医学报, 2016, 47(1): 25-33.

SHU Jing-ting，ZHANG Ming，SONG Chi，SHAN Yan-ju，XU Wen-juan，SONG Wei-tao，LI Hui-fang. Identification of Differentially Expressed Genes for Myofiber Characteristics in Soleus Muscles between Qingyuan Partridge Chickens and Cobb Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 25-33.

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基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因

Identification of Differentially Expressed Genes for Myofiber Characteristics in Soleus Muscles between Qingyuan Partridge Chickens and Cobb Broilers

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