IFN-γ对羊驼皮肤黑色素细胞增殖和黑色素合成的影响

doi:10.11843/j.issn.0366-6964.2015.12.010

摘要/Abstract

摘要：

旨在研究IFN-γ 对黑色素细胞增殖及黑色素合成的影响。不同浓度 IFN-γ （0.0、0.5、2.0、8.0、16.0、32.0、64.0 ng•mL-1）分别处理羊驼皮肤黑色素细胞24、48和72 h。MTT 法检测黑色素细胞活力，紫外分光光度法检测黑色素含量，qRT-PCR 检测 MC1R、TYR、TYRP1、MITF、DCT 及 NOS2 mRNA 水平的变化。结果显示，24 h 黑色素细胞活力受到抑制，72 h 细胞活力恢复且黑色素合成增强；IFN-γ 可促进黑色素含量的增加，以 32.0 ng•mL-1 IFN-γ 增加极显著。 TYRP1、MITF、DCT、NOS2 mRNA 相对表达量显著增加（P<0.05），MC1R、TYR mRNA 相对表达量增加不显著（P>0.05）。IFN-γ 可促进黑色素细胞树突增长，可能有助于黑色素的转运；IFN-γ诱导黑色素的合成，可能与黑色素细胞中 NOS2 和 MITF 的高表达有关，可能通过 NO/cGMP/PKG 介导的信号通路合成黑色素。

关键词: IFN-&gamma, 羊驼, 黑色素细胞, 黑色素, 增殖

Abstract:

The effect of IFN-γ on melanocytes proliferation and melanogenesis was studied.Different concentrations of IFN-γ (0.0，0.5，2.0，8.0，16.0，32.0，64.0 ng•mL^-1) treated separately melanocytes of Alpaca skin at 24，48，72 h.The activity of melanocytes was detected using MTT method，the melanin content was tested with ultraviolet spectrophotometric method and qRT-PCR was used to detect the changes of MC1R，TYR，TYRP1，MITF，DCT and NOS2 on mRNA level.The results showed that the melanocytes activity was inhibited after 24 h，melanocytes activity recovered and the melanogenesis was enhanced after 72 h.IFN-γ promoted the increase of melanin content and 32.0 ng•mL^-1 IFN-γ increased significantly.The relative mRNA expression increased significantly on TYRP1，MITF，DCT，NOS2 (P<0.05)，but there were no significant differences on MC1R，TYR (P>0.05).IFN-γ promoted the growth of melanocyte dendrites，which may contribute to melanin transport；IFN-γ induced melanogenesis，which may be related to NOS2 and MITF high expression in melanoma cells and the melanin may be produced through NO/cGMP/PKG-mediated signal pathway.

Key words: IFN-γ, alpaca, melanocytes, melanin, proliferation

中图分类号:

Q343

赵益文，赵礼军，李振，尹志红，白瑞，赵敏，庞全海. IFN-γ对羊驼皮肤黑色素细胞增殖和黑色素合成的影响[J]. 畜牧兽医学报, 2015, 46(12): 2192-2198.

ZHAO Yi-wen，ZHAO Li-jun，LI Zhen，YIN Zhi-hong，BAI Rui，ZHAO Min，PANG Quan-hai. The Effect of IFN-γ on Melanocytes Proliferation and Melanogenesis of Alpaca Skin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2192-2198.

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